Search Results - "Themis, Michael"

Three-dimensional cell culture: from evolution to revolution by Alhaque, Sharmin, Themis, Michael, Rashidi, Hassan

“…Recent advances in the isolation of tissue-resident adult stem cells and the identification of inductive factors that efficiently direct differentiation of…”
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Widespread Distribution and Muscle Differentiation of Human Fetal Mesenchymal Stem Cells After Intrauterine Transplantation in Dystrophic mdx Mouse by Chan, Jerry, Waddington, Simon N., O'Donoghue, Keelin, Kurata, Hitoshi, Guillot, Pascale V., Gotherstrom, Cecilia, Themis, Michael, Morgan, Jennifer E., Fisk, Nicholas M.

“…Duchenne muscular dystrophy (DMD) is a common X‐linked disease resulting from the absence of dystrophin in muscle. Affected boys suffer from incurable…”
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Differentiation of human fetal mesenchymal stem cells into cells with an oligodendrocyte phenotype by Kennea, Nigel L., Waddington, Simon N., Chan, Jerry, O'Donoghue, Keelin, Yeung, Davy, Taylor, Deanna L., Al-Allaf, Faisal A., Pirianov, Grisha, Themis, Michael, Edwards, A. David, Fisk, Nicholas M., Mehmet, Huseyin

“…The potential of mesenchymal stem cells (MSC) to differentiate into neural lineages has raised the possibility of autologous cell transplantation as a therapy…”
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Lentivector Producer Cell Lines with Stably Expressed Vesiculovirus Envelopes by Tijani, Maha, Munis, Altar M., Perry, Christopher, Sanber, Khaled, Ferraresso, Marta, Mukhopadhyay, Tarit, Themis, Michael, Nisoli, Ilaria, Mattiuzzo, Giada, Collins, Mary K., Takeuchi, Yasuhiro

“…Retroviral and lentiviral vectors often use the envelope G protein from the vesicular stomatitis virus Indiana strain (VSVind.G). However, lentivector producer…”
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Modifying inter-cistronic sequence significantly enhances IRES dependent second gene expression in bicistronic vector: Construction of optimised cassette for gene therapy of familial hypercholesterolemia by Al-Allaf, Faisal A., Abduljaleel, Zainularifeen, Athar, Mohammad, Taher, Mohiuddin M., Khan, Wajahatullah, Mehmet, Huseyin, Colakogullari, Mukaddes, Apostolidou, Sophia, Bigger, Brian, Waddington, Simon, Coutelle, Charles, Themis, Michael, Al-Ahdal, Mohammed N., Al-Mohanna, Futwan A., Al-Hassnan, Zuhair N., Bouazzaoui, Abdellatif

“…Internal ribosome entry site (IRES) sequences have become a valuable tool in the construction of gene transfer and therapeutic vectors for multi-cistronic gene…”
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LDLR-Gene therapy for familial hypercholesterolaemia: problems, progress, and perspectives by Al-Allaf, Faisal A, Coutelle, Charles, Waddington, Simon N, David, Anna L, Harbottle, Richard, Themis, Michael

“…Coronary artery diseases (CAD) inflict a heavy economical and social burden on most populations and contribute significantly to their morbidity and mortality…”
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Three-dimensional cell culture: from evolution to revolution by Alhaque, Sharmin, Themis, Michael, Rashidi, Hassan

“…Recent advances in the isolation of tissue-resident adult stem cells and the identification of inductive factors that efficiently direct differentiation of…”
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Overexpression of connexin 43 using a retroviral vector improves electrical coupling of skeletal myoblasts with cardiac myocytes in vitro by Tolmachov, Oleg, Ma, Yu-Ling, Themis, Michael, Patel, Pravina, Spohr, Hilmar, Macleod, Kenneth T, Ullrich, Nina D, Kienast, Yvonne, Coutelle, Charles, Peters, Nicholas S

“…Organ transplantation is presently often the only available option to repair a damaged heart. As heart donors are scarce, engineering of cardiac grafts from…”
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Optimised protocols to generate high titre lentiviral vectors using a novel transfection agent enabling extended HEK293T culture following transient transfection and suspension culture by Suleman, Saqlain, Fawaz, Serena, Roberts, Terry, Ellison, Stuart, Bigger, Brian, Themis, Michael

“…HIV-1 based lentiviral viruses are considered powerful and versatile gene therapy vectors to deliver therapeutic genes to patients with hereditary or acquired…”
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Ultrasonographic Development of the Fetal Sheep Stomach and Evaluation of Early Gestation Ultrasound-guided In Utero Intragastric Injection by David, Anna L, Abi-Nader, Khalil N, Weisz, Boaz, Shaw, S.W. Steven, Themis, Michael, Cook, Terence, Coutelle, Charles, Rodeck, Charles H, Peebles, Donald M

“…Summary Objective Safely targeting the fetal gastrointestinal tract during early gestation is essential to develop effective prenatal gene therapy for…”
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Rapid and inexpensive purification of adenovirus vectors using an optimised aqueous two-phase technology by Suleman, Saqlain, Schrubaji, Kuteiba, Filippou, Chrysovalanto, Ignatova, Svetlana, Hewitson, Peter, Huddleston, Jonathan, Karda, Rajvinder, Waddington, Simon N., Themis, Michael

“…[Display omitted] •Adenovirus (AdV) vectors are used in vaccine development and gene therapy.•AdV Research and Master Virus Seed Stocks (RVSS/MVSS) are…”
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Genome wide classification and characterisation of CpG sites in cancer and normal cells by Ghorbani, Mohammadmersad, Themis, Michael, Payne, Annette

“…Abstract This study identifies common methylation patterns across different cancer types in an effort to identify common molecular events in diverse types of…”
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HIV- 1 lentivirus tethering to the genome is associated with transcription factor binding sites found in genes that favour virus survival by Suleman, Saqlain, Payne, Annette, Bowden, Johnathan, Haque, Sharmin Al, Zahn, Marco, Fawaz, Serena, Khalifa, Mohammad S., Jobling, Susan, Hay, David, Franco, Matteo, Fronza, Raffaele, Wang, Wei, Strobel-Freidekind, Olga, Deichmann, Annette, Takeuchi, Yasuhiro, Waddington, Simon N., Gil-Farina, Irene, Schmidt, Manfred, Themis, Michael

“…Lentiviral vectors (LV) are attractive for permanent and effective gene therapy. However, integration into the host genome can cause insertional mutagenesis…”
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Improving the Assessment of Risk Factors Relevant to Potential Carcinogenicity of Gene Therapies: A Consensus Article by Klapwijk, Jan C, Del Rio Espinola, Alberto, Libertini, Silvana, Collin, Philippe, Fellows, Mick D, Jobling, Susan, Lynch, Anthony M, Martus, HansJoerg, Vickers, Catherine, Zeller, Andreas, Biasco, Luca, Brugman, Martijn H, Bushmann, Frederic D, Cathomen, Toni, Ertl, Hildegrund C J, Gabriel, Richard, Gao, Guangping, Jadlowsky, Julie K, Kimber, Ian, Lanz, Thomas A, Levine, Bruce L, Micklethwaite, Kenneth P, Onodera, Masafumi, Pizzurro, Daniella M, Reed, Simon, Rothe, Michael, Sabatino, Denise E, Salk, Jesse J, Schambach, Axel, Themis, Michael, Yuan, Jing

“…Regulators and industry are actively seeking improvements and alternatives to current models and approaches to evaluate potential carcinogenicity of gene…”
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Monitoring for potential adverse effects of prenatal gene therapy: genotoxicity analysis in vitro and on small animal models ex vivo and in vivo by Themis, Michael

“…Gene delivery by integrating vectors has the potential to cause genotoxicity in the host by insertional mutagenesis (IM). Previously, the risk of IM by…”
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Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells by Lucendo-Villarin, Balta, Rashidi, Hassan, Alhaque, Sharmin, Fischer, Lena, Meseguer-Ripolles, Jose, Wang, Yu, O'Farrelly, Cliona, Themis, Michael, Hay, David C

“…The development of renewable sources of liver tissue is required to improve cell-based modelling, and develop human tissue for transplantation. Human embryonic…”
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Transduction of Fetal Mice With a Feline Lentiviral Vector Induces Liver Tumors Which Exhibit an E2F Activation Signature by Condiotti, Reba, Goldenberg, Daniel, Giladi, Hilla, Schnitzer-Perlman, Temima, Waddington, Simon N, Buckley, Suzanne MK, Heim, Denise, Cheung, Wing, Themis, Matthew, Coutelle, Charles, Simerzin, Alina, Osejindu, Emma, Wege, Henning, Themis, Michael, Galun, Eithan

“…Lentiviral vectors are widely used in basic research and clinical applications for gene transfer and long-term expression; however, safety issues have not yet…”
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Monitoring for potential adverse effects of prenatal gene therapy: mouse models for developmental aberrations and inadvertent germ line transmission by Coutelle, Charles, Waddington, Simon N, Themis, Michael

“…So far no systematic studies have been conducted to investigate developmental aberrations after prenatal gene transfer in mice. Here, we suggest procedures for…”
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Restoration of LDL receptor function in cells from patients with autosomal recessive hypercholesterolemia by retroviral expression of ARH1 by Eden, Emily R, Patel, Dilipkumar D, Sun, Xi-Ming, Burden, Jemima J, Themis, Michael, Edwards, Matthew, Lee, Philip, Neuwirth, Clare, Naoumova, Rossitza P, Soutar, Anne K

“…Familial hypercholesterolemia is an autosomal dominant disorder with a gene-dosage effect that is usually caused by mutations in the LDL receptor gene that…”
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In Utero gene therapy: current challenges and perspectives by Waddington, Simon N., Kramer, M. Gabriela, Hernandez-Alcoceba, Ruben, Buckley, Suzanne M.K., Themis, Michael, Coutelle, Charles, Prieto, Jesus

“…Over the past few years, considerable progress in prenatal diagnosis and surgery combined with improvements in vector design vindicate a reappraisal of the…”
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