Search Results - "Theile, Christopher S"

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions by Guimaraes, Carla P, Witte, Martin D, Theile, Christopher S, Bozkurt, Gunes, Kundrat, Lenka, Blom, Annet E M, Ploegh, Hidde L

“…Methods for site-specific modification of proteins should be quantitative and versatile with respect to the nature and size of the biological or chemical…”
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siRNA carrying an (E)-vinylphosphonate moiety at the 5΄ end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2 by Elkayam, Elad, Parmar, Rubina, Brown, Christopher R, Willoughby, Jennifer L, Theile, Christopher S, Manoharan, Muthiah, Joshua-Tor, Leemor

“…Efficient gene silencing by RNA interference (RNAi) in vivo requires the recognition and binding of the 5΄- phosphate of the guide strand of an siRNA by the…”
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Evaluating the oral delivery of GalNAc-conjugated siRNAs in rodents and non-human primates by Yu, Mikyung, Qin, June, Liu, Xiumin, Ramsden, Diane, Williams, Brian, Zlatev, Ivan, Guenther, Dale, Matsuda, Shigeo, Tymon, Roxanne, Darcy, Justin, Wong, Catrina, Tsung, Jamie, Zawaneh, Peter, Chong, Saeho, Theile, Christopher S, Taneja, Nathan, Rogers, Arlin, Liu, Ju, Castellanos-Rizaldos, Elena, Bond, Sarah, So, Kawai, Denoncourt, Jason, Castoreno, Adam, Manoharan, Muthiah, Wu, Jing-Tao, Fitzgerald, Kevin, Maier, Martin A, Jadhav, Vasant, Nair, Jayaprakash K

“…Oral delivery is the most widely used and convenient route of administration of medicine. However, oral administration of hydrophilic macromolecules is…”
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5′-(E)-Vinylphosphonate: A Stable Phosphate Mimic Can Improve the RNAi Activity of siRNA-GalNAc Conjugates by Parmar, Rubina, Willoughby, Jennifer L. S., Liu, Jingxuan, Foster, Donald J., Brigham, Benjamin, Theile, Christopher S., Charisse, Klaus, Akinc, Akin, Guidry, Erin, Pei, Yi, Strapps, Walter, Cancilla, Mark, Stanton, Matthew G., Rajeev, Kallanthottathil G., Sepp-Lorenzino, Laura, Manoharan, Muthiah, Meyers, Rachel, Maier, Martin A., Jadhav, Vasant

“…Small interfering RNA (siRNA)‐mediated silencing requires siRNA loading into the RNA‐induced silencing complex (RISC). Presence of 5′‐phosphate (5′‐P) is…”
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Facile Synthesis, Geometry, and 2′-Substituent-Dependent in Vivo Activity of 5′‑(E)- and 5′‑(Z)‑Vinylphosphonate-Modified siRNA Conjugates by Parmar, Rubina Giare, Brown, Christopher R, Matsuda, Shigeo, Willoughby, Jennifer L. S, Theile, Christopher S, Charissé, Klaus, Foster, Donald J, Zlatev, Ivan, Jadhav, Vasant, Maier, Martin A, Egli, Martin, Manoharan, Muthiah, Rajeev, Kallanthottathil G

“…(E)-Vinylphosphonate ((E)-VP), a metabolically stable phosphate mimic at the 5′-end of the antisense strand, enhances the in vivo potency of siRNA. Here we…”
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Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling by Jahan, Akhee S., Lestra, Maxime, Swee, Lee Kim, Fan, Ying, Lamers, Mart M., Tafesse, Fikadu G., Theile, Christopher S., Spooner, Eric, Bruzzone, Roberto, Ploegh, Hidde L., Sanyal, Sumana

“…Posttranslational modifications are central to the spatial and temporal regulation of protein function. Among others, phosphorylation and ubiquitylation are…”
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Monovalent engagement of the BCR activates ovalbumin-specific transnuclear B cells by Avalos, Ana M, Bilate, Angelina M, Witte, Martin D, Tai, Albert K, He, Jiang, Frushicheva, Maria P, Thill, Peter D, Meyer-Wentrup, Friederike, Theile, Christopher S, Chakraborty, Arup K, Zhuang, Xiaowei, Ploegh, Hidde L

“…Valency requirements for B cell activation upon antigen encounter are poorly understood. OB1 transnuclear B cells express an IgG1 B cell receptor (BCR)…”
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Site-specific chemoenzymatic labeling of aerolysin enables the identification of new aerolysin receptors by Wuethrich, Irene, Peeters, Janneke G C, Blom, Annet E M, Theile, Christopher S, Li, Zeyang, Spooner, Eric, Ploegh, Hidde L, Guimaraes, Carla P

“…Aerolysin is a secreted bacterial toxin that perforates the plasma membrane of a target cell with lethal consequences. Previously explored native and…”
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An efficient synthetic approach to 6,5'-(S)- and 6,5'-(R)-cyclouridine by Theile, Christopher S, McLaughlin, Larry W

“…Here we present new routes for the efficient syntheses of 6,5'-(S)- and 6,5'-(R)-cyclouridine. The syntheses utilize readily accessible uridine as a starting…”
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Expanding RNAi therapeutics to extrahepatic tissues with lipophilic conjugates by Brown, Kirk M., Nair, Jayaprakash K., Janas, Maja M., Anglero-Rodriguez, Yesseinia I., Dang, Lan T. H., Peng, Haiyan, Theile, Christopher S., Castellanos-Rizaldos, Elena, Brown, Christopher, Foster, Donald, Kurz, Jeffrey, Allen, Jeffrey, Maganti, Rajanikanth, Li, Jing, Matsuda, Shigeo, Stricos, Matthew, Chickering, Tyler, Jung, Michelle, Wassarman, Kelly, Rollins, Jeff, Woods, Lauren, Kelin, Alex, Guenther, Dale C., Mobley, Melissa W., Petrulis, John, McDougall, Robin, Racie, Timothy, Bombardier, Jessica, Cha, Diana, Agarwal, Saket, Johnson, Lei, Jiang, Yongfeng, Lentini, Scott, Gilbert, Jason, Nguyen, Tuyen, Chigas, Samantha, LeBlanc, Sarah, Poreci, Urjana, Kasper, Anne, Rogers, Arlin B., Chong, Saeho, Davis, Wendell, Sutherland, Jessica E., Castoreno, Adam, Milstein, Stuart, Schlegel, Mark K., Zlatev, Ivan, Charisse, Klaus, Keating, Mark, Manoharan, Muthiah, Fitzgerald, Kevin, Wu, Jing-Tao, Maier, Martin A., Jadhav, Vasant

“…Therapeutics based on short interfering RNAs (siRNAs) delivered to hepatocytes have been approved, but new delivery solutions are needed to target additional…”
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Site-specific N-terminal labeling of proteins using sortase-mediated reactions by Theile, Christopher S, Witte, Martin D, Blom, Annet E M, Kundrat, Lenka, Ploegh, Hidde L, Guimaraes, Carla P

“…This protocol describes the use of sortase-mediated reactions to label the N terminus of any given protein of interest. The sortase recognition sequence, LPXTG…”
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Turnip yellow mosaic virus protease binds ubiquitin suboptimally to fine-tune its deubiquitinase activity by Fieulaine, Sonia, Witte, Martin D., Theile, Christopher S., Ayach, Maya, Ploegh, Hidde L., Jupin, Isabelle, Bressanelli, Stéphane

“…Single-stranded, positive-sense RNA viruses assemble their replication complexes in infected cells from a multidomain replication polyprotein. This polyprotein…”
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Rational optimization of siRNA to ensure strand bias in the interaction with the RNA-induced silencing complex by Datta, Dhrubajyoti, Theile, Christopher S, Wassarman, Kelly, Qin, June, Racie, Tim, Schmidt, Karyn, Jiang, Yongfeng, Sigel, Rachel, Janas, Maja M, Egli, Martin, Manoharan, Muthiah

“…To ensure specificity of small interfering RNAs (siRNAs), the antisense strand must be selected by the RNA-induced silencing complex (RISC). We have previously…”
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Centyrin ligands for extrahepatic delivery of siRNA by Klein, Donna, Goldberg, Shalom, Theile, Christopher S., Dambra, Richard, Haskell, Kathleen, Kuhar, Elise, Lin, Tricia, Parmar, Rubina, Manoharan, Muthiah, Richter, Mark, Wu, Meizhen, Mendrola Zarazowski, Jeannine, Jadhav, Vasant, Maier, Martin A., Sepp-Lorenzino, Laura, O’Neil, Karyn, Dudkin, Vadim

“…RNA interference (RNAi) offers the potential to treat disease at the earliest onset by selectively turning off the expression of target genes, such as…”
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Single-Stranded Hairpin Loop RNAs (loopmeRNAs) Potently Induce Gene Silencing through the RNA Interference Pathway by Aluri, Krishna C., Datta, Dhrubajyoti, Waldron, Scott, Taneja, Nate, Qin, June, Donnelly, Daniel P., Theile, Christopher S., Guenther, Dale C., Lei, Li, Harp, Joel M., Pallan, Pradeep S., Egli, Martin, Zlatev, Ivan, Manoharan, Muthiah

“…Synthetic small interfering RNAs conjugated to trivalent N-acetylgalactosamine (GalNAc) are clinically validated drugs for treatment of liver diseases…”
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Shorter Is Better: The α‑(l)‑Threofuranosyl Nucleic Acid Modification Improves Stability, Potency, Safety, and Ago2 Binding and Mitigates Off-Target Effects of Small Interfering RNAs by Matsuda, Shigeo, Bala, Saikat, Liao, Jen-Yu, Datta, Dhrubajyoti, Mikami, Atsushi, Woods, Lauren, Harp, Joel M., Gilbert, Jason A., Bisbe, Anna, Manoharan, Rajar M., Kim, MaryBeth, Theile, Christopher S., Guenther, Dale C., Jiang, Yongfeng, Agarwal, Saket, Maganti, Rajanikanth, Schlegel, Mark K., Zlatev, Ivan, Charisse, Klaus, Rajeev, Kallanthottathil G., Castoreno, Adam, Maier, Martin, Janas, Maja M., Egli, Martin, Chaput, John C., Manoharan, Muthiah

“…Chemical modifications are necessary to ensure the metabolic stability and efficacy of oligonucleotide-based therapeutics. Here, we describe analyses of the…”
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Site-specific protein modification using immobilized sortase in batch and continuous-flow systems by Witte, Martin D, Wu, Tongfei, Guimaraes, Carla P, Theile, Christopher S, Blom, Annet E M, Ingram, Jessica R, Li, Zeyang, Kundrat, Lenka, Goldberg, Shalom D, Ploegh, Hidde L

“…Sortase A can be used for the site-specific modification of proteins. This protocol describes its immobilization on Sepharose beads, allowing for larger-scale…”
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Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry by Witte, Martin D, Theile, Christopher S, Wu, Tongfei, Guimaraes, Carla P, Blom, Annet E M, Ploegh, Hidde L

“…Chimeric proteins, including bispecific antibodies, are biological tools with therapeutic applications. Genetic fusion and ligation methods allow the creation…”
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A catalytic independent function of the deubiquitinating enzyme USP14 regulates hippocampal synaptic short‐term plasticity and vesicle number by Walters, Brandon J., Hallengren, Jada J., Theile, Christopher S., Ploegh, Hidde L., Wilson, Scott M., Dobrunz, Lynn E.

“…Key points Mice carrying the ataxia (axJ) mutation have a 95% reduction in the deubiquitinating enzyme USP14, which results in a reduction in hippocampal…”
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siRNA carrying an (E)-vinylphosphonate moiety at the 5΄ end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2 by Elkayam, Elad, Parmar, Rubina, Brown, Christopher R, Willoughby, Jennifer L, Theile, Christopher S, Manoharan, Muthiah, Joshua-Tor, Leemor

“…Efficient gene silencing by RNA interference (RNAi) in vivo requires the recognition and binding of the 5'- phosphate of the guide strand of an siRNA by the…”
Get full text