Search Results - "Thamm, Iris"

Use of an ALFexpress DNA sequencer to analyze protein-nucleic acid interactions by band shift assay by Filée, P, Delmarcelle, M, Thamm, I, Joris, B

“…Gel retardation analysis, or band shift assay, is technically the simplest method to investigate protein-nucleic acid interactions. In this report, we describe…”
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Dimerization and DNA Binding Properties of theBacillus licheniformis 749/I BlaI Repressor by Filée, Patrice, Vreuls, Christelle, Herman, Raphaël, Thamm, Iris, Aerts, Tony, De Deyn, Peter P., Frère, Jean-Marie, Joris, Bernard

“…In the absence of penicillin, the औ-lactamase encoding gene blaP of Bacillus licheniformis749/I is negatively regulated by the transcriptional repressor BlaI…”
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Probing the Specificity of the Subclass B3 FEZ-1 Metallo-β-lactamase by Site-directed Mutagenesis by Mercuri, Paola Sandra, García-Sáez, Isabel, De Vriendt, Kris, Thamm, Iris, Devreese, Bart, Van Beeumen, Jozef, Dideberg, Otto, Rossolini, Gian Maria, Frère, Jean-Marie, Galleni, Moreno

“…The subclass B3 FEZ-1 β-lactamase produced by Fluoribacter (Legionella) gormanii is a Zn(II)-containing enzyme that hydrolyzes the β-lactam bond in…”
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The penicillin resistance of Enterococcus faecalis JH2-2r results from an overproduction of the low-affinity penicillin-binding protein PBP4 and does not involve a psr-like gene by Duez, Colette, Zorzi, Willy, Sapunaric, Frederic, Amoroso, Ana, Thamm, Iris, Coyette, Jacques

“…Centre d’Ingénierie des Protéines, Institut de Chimie, B6, Université de Liège, (Sart Tilman), B-4000 Liège, Belgium 1 Author for correspondence: Jacques…”
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Dimerization and DNA binding properties of the Bacillus licheniformis 749/I BlaI repressor by Filée, Patrice, Vreuls, Christelle, Herman, Raphaël, Thamm, Iris, Aerts, Tony, De Deyn, Peter P, Frère, Jean-Marie, Joris, Bernard

“…In the absence of penicillin, the beta-lactamase encoding gene blaP of Bacillus licheniformis 749/I is negatively regulated by the transcriptional repressor…”
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Probing the Specificity of the Subclass B3 FEZ-1 Metallo-β-lactamase by Site-directed Mutagenesis by Paola Sandra Mercuri, Isabel García-Sáez, Kris De Vriendt, Iris Thamm, Bart Devreese, Jozef Van Beeumen, Otto Dideberg, Gian Maria Rossolini, Jean-Marie Frère, Moreno Galleni

“…The subclass B3 FEZ-1 β-lactamase produced by Fluoribacter ( Legionella ) gormanii is a Zn(II)-containing enzyme that hydrolyzes the β-lactam bond in…”
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The penicillin resistance of Enterococcus faecalis JH2-2r results from an overproduction of the low-affinity penicillin-binding protein PBP4 and does not involve a psr-like gene The GenBank accession numbers for the sequences reported in this paper are Y17797 for the 8·4 kb segment of pDML521; AJ290435 for pbp4 of E. faecalis JH2-2; AJ276231 and AJ276232 for the psr-like gene of E. faecalis JH2-2 or JH2-2r, respectively by Duez, Colette, Zorzi, Willy, Sapunaric, Frédéric, Amoroso, Ana, Thamm, Iris, Coyette, Jacques

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Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters by Ledent, Philippe, Duez, Colette, Vanhove, Marc, Lejeune, Annabelle, Fonzé, Eveline, Charlier, Paulette, Rhazi-Filali, Fouzia, Thamm, Iris, Guillaume, Gilliane, Samyn, Bart, Devreese, Bart, Van Beeumen, Jozef, Lamotte-Brasseur, Josette, Frère, Jean-Marie

“…The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the β-lactamase of a thermophilic Bacillus…”
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Two new aminopeptidases from Ochrobactrum anthropi active on D-alanyl-p-nitroanilide by Fanuel, L, Thamm, I, Kostanjevecki, V, Samyn, B, Joris, B, Goffin, C, Brannigan, J, Van Beeumen, J, Frère, J M

“…Two new enzymes which hydrolyse D-alanyl-p-nitroanilide have been detected in Ochrobactrum anthropi LMG7991 extracts. The first enzyme, DmpB, was purified to…”
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FEMS Microbiology Letters by Brans, Alain, Loriaux, Axelle, Thamm, Iris, Joris, Bernard, Dusart, Jean

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Structure of In31, abla IMP-Containing Pseudomonas aeruginosa Integron Phyletically Related to In5, Which Carries an Unusual Array of Gene Cassettes by Nezha Laraki, Moreno Galleni, Iris Thamm, Maria Letizia Riccio, Gianfranco Amicosante, Jean-Marie Frère, Gian Maria Rossolini

“…Classifications Services AAC Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Structure of In31, a bla IMP -Containing Pseudomonas aeruginosa Integron Phyletically Related to In5, Which Carries an Unusual Array of Gene Cassettes by Laraki, Nezha, Galleni, Moreno, Thamm, Iris, Riccio, Maria Letizia, Amicosante, Gianfranco, Frère, Jean-Marie, Rossolini, Gian Maria

“…ABSTRACT The location and environment of the acquired bla IMP gene, which encodes the IMP-1 metallo-β-lactamase, were investigated in a Japanese Pseudomonas…”
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Structure of In31, ablaIMP-Containing Pseudomonas aeruginosa Integron Phyletically Related to In5, Which Carries an Unusual Array of Gene Cassettes by Laraki, Nezha, Galleni, Moreno, Thamm, Iris, Riccio, Maria Letizia, Amicosante, Gianfranco, Frère, Jean-Marie, Rossolini, Gian Maria

“…The location and environment of the acquiredblaIMP gene, which encodes the IMP-1 metallo-β-lactamase, were investigated in a JapanesePseudomonas aeruginosa…”
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Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon by Brans, Alain, Loriaux, Axelle, Thamm, Iris, Joris, Bernard, Dusart, Jean

“…The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA…”
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Structure of In31, a blaIMP-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes by LARAKI, N, GALLENI, M, THAMM, I, RICCIO, M. L, AMICOSANTE, G, FRERE, J.-M, ROSSOLINI, G. M

“…The location and environment of the acquired blaIMP gene, which encodes the IMP-1 metallo-beta-lactamase, were investigated in a Japanese Pseudomonas…”
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Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate by Malissard, M, Duez, C, Guinand, M, Vacheron, M J, Michel, G, Marty, N, Joris, B, Thamm, I, Ghuysen, J M

“…The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl…”
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Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon by Brans, Alain, Loriaux, Axelle, Thamm, Iris, Joris, Bernard, Dusart, Jean

“…Abstract The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated…”
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A new, highly sensitive method for the detection and quantification of penicillin-binding proteins by GALLENI, M, LAKAYE, B, LEPAGE, S, JAMIN, M, THAMM, I, JORIS, B, FRERE, J.-M

“…A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the…”
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Kinetic properties of the Bacillus licheniformis penicillin-binding proteins by Lepage, S, Galleni, M, Lakaye, B, Joris, B, Thamm, I, Frere, J M

“…In the analysis of the interactions between beta-lactam antibiotics and their target enzymes, it is often difficult to estimate the kinetic properties of the…”
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Saturation of penicillin-binding protein 1 by beta-lactam antibiotics in growing cells of Bacillus licheniformis by Lepage, S, Lakaye, B, Galleni, M, Thamm, I, Crine, M, Groslambert, S, Frère, J M

“…With the help of a new highly sensitive method allowing the quantification of free penicillin-binding proteins (PBPs) and of an integrated mathematical model,…”
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