The lipopolysaccharide biosynthesis locus of Campylobacter jejuni 81116

Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands Department of Applied Biology, RMIT GPO Box 2476V, Melbourne 3001, Australia ABSTRACT Most Campylobacter jejuni strains expres...

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Published in:	Microbiology (Society for General Microbiology) Vol. 144; no. 8; pp. 2049 - 2061
Main Authors:	Fry, Ben N, Korolik, Victoria, ten Brinke, Janna A, Pennings, Maarten T. T, Zalm, Robert, Teunis, Bart J. J, Coloe, Peter J, van der Zeijst, Bernard A. M
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-08-1998 Society for General Microbiology
Subjects:	Acetyltransferases - genetics Amino Acid Sequence ATP-Binding Cassette Transporters - genetics Bacterial Proteins Bacteriology Biological and medical sciences Campylobacter hyoilei Campylobacter jejuni Campylobacter jejuni - genetics Campylobacter jejuni - metabolism Carbohydrate Epimerases - genetics DNA, Bacterial - isolation & purification Escherichia coli Proteins Fundamental and applied biological sciences. Psychology Galactosyltransferases - genetics Genes, Bacterial Genetics Glycosyltransferases - genetics Lipopolysaccharides - biosynthesis Lipopolysaccharides - chemistry Membrane Proteins - genetics Methyl-Accepting Chemotaxis Proteins Microbiology Molecular Sequence Data Multigene Family Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Transaminases - genetics Transferases (Other Substituted Phosphate Groups) - genetics UDPglucose 4-Epimerase - genetics Characterization Gene cluster Enzyme Campylobacteraceae Lipopolysaccharide Bacteria Biosynthesis Protein Structural analysis Campylobacter jejuni
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Summary:	Department of Bacteriology, Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands Department of Applied Biology, RMIT GPO Box 2476V, Melbourne 3001, Australia ABSTRACT Most Campylobacter jejuni strains express lipo-oligosaccharides. Some strains also express lipopolysaccharides (LPS), with O-antigen-like carbohydrate repeats. C. jejuni 81116 expresses an LPS containing both lipo-oligosaccharides and O-antigen-like repeats, but nothing is known about the structure or sugar composition of these LPS species. A cosmid library of the genome of C. jejuni 81116 was constructed and probed with Campylobacter hyoilei genes involved in LPS synthesis. Five cosmids hybridized with the probe and two of these expressed C. jejuni 81116 LPS in Escherichia coli . By subcloning, a 16 kb DNA region was identified which contains the genetic information required to express C. jejuni LPS. DNA sequence analysis revealed 11 ORFs homologous to genes involved in LPS synthesis of other bacteria. They consisted of three homologues of sugar biosynthesis genes, two homologues of transport genes and six homologues of sugar transferases. * Author for correspondence: Bernard A. M. van der Zeijst. Tel: +31 30 274 41 64. Fax: +31 30 274 44 39. e-mail: ben.van.der.zeijst@rivm.nl Keywords: Campylobacter jejuni, rfb gene cluster, lipo-oligosaccharide (LOS), lipo-polysaccharide (LPS), O-antigen Present address: National Institute of Public Health and the Environment, Vaccine Division, PO Box 1, 3720 Bilthoven, The Netherlands. Present address: Department of Applied Biology and Biotechnology, Royal Melbourne Institute of Technology, 124 LaTrobe St, Melbourne, VIC 3000, Australia.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1350-0872 1465-2080
DOI:	10.1099/00221287-144-8-2049