Search Results - "Tetin, Sergey Y."

Affinity of anti-spike antibodies in SARS-CoV-2 patient plasma and its effect on COVID-19 antibody assays by Macdonald, Patrick J., Ruan, Qiaoqiao, Grieshaber, Jessica L., Swift, Kerry M., Taylor, Russell E., Prostko, John C., Tetin, Sergey Y.

“…Measuring anti-spike protein antibodies in human plasma or serum is commonly used to determine prior exposure to SARS-CoV-2 infection and to assess the…”
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Affinity of anti-spike antibodies to three major SARS-CoV-2 variants in recipients of three major vaccines by Macdonald, Patrick J., Schaub, Jeffrey M., Ruan, Qiaoqiao, Williams, Carroll L., Prostko, John C., Tetin, Sergey Y.

“…Background Measuring anti-viral antibody affinity in blood plasma or serum is a rational quantitative approach to assess humoral immune response and acquired…”
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Applications of dual-color fluorescence cross-correlation spectroscopy in antibody binding studies by Ruan, Qiaoqiao, Tetin, Sergey Y.

“…Two-photon dual-color fluorescence cross-correlation spectroscopy (DC–FCCS) was applied to study the binding interactions of monoclonal antibodies (mAbs) and…”
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Water Channel in the Binding Site of a High Affinity Anti-Methotrexate Antibody by Gayda, Susan, Longenecker, Kenton L, Manoj, Sharmila, Judge, Russell A, Saldana, Sylvia C, Ruan, Qiaoqiao, Swift, Kerry M, Tetin, Sergey Y

“…In the present study, we report the structure of the free and drug-bound Fab fragment of a high affinity anti-methotrexate antibody and perform a thermodynamic…”
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Epifluorescent single-molecule counting with Streptavidin-Phycoerythrin conjugates by Schaub, Jeffrey M., Ruan, Qiaoqiao, Tetin, Sergey Y.

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Epifluorescent single-molecule counting with Streptavidin-Phycoerythrin conjugates by Schaub, Jeffrey M., Ruan, Qiaoqiao, Tetin, Sergey Y.

“…Single-molecule methods, specifically single-molecule counting, convey high sensitivity in research applications. However, single-molecule counting experiments…”
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Direct single-molecule imaging for diagnostic and blood screening assays by Ruan, Qiaoqiao, Macdonald, Patrick J., Swift, Kerry M., Tetin, Sergey Y.

“…Every year, over 100 million units of donated blood undergo mandatory screening for HIV, hepatitis B, hepatitis C, and syphilis worldwide. Often, donated blood…”
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Method for direct measuring antibody affinity in biological sample by Ruan, Qiaoqiao, Schaub, Jeffrey M., Macdonald, Patrick J., Tetin, Sergey Y.

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Rhodamine-Derived Fluorescent Dye with Inherent Blinking Behavior for Super-Resolution Imaging by Macdonald, Patrick J, Gayda, Susan, Haack, Richard A, Ruan, Qiaoqiao, Himmelsbach, Richard J, Tetin, Sergey Y

“…Super-resolution microscopy enables imaging of structures smaller than the diffraction limit. Single-molecule localization microscopy methods, such as…”
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Direct single-molecule counting for immunoassay applications by Macdonald, Patrick J., Ruan, Qiaoqiao, Tetin, Sergey Y.

“…Single-molecule methods offer specificity in studying complex systems and dynamics, but they also offer high sensitivity for basic enumeration. We apply…”
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Single-cell clinical chemistry: Exploring glucose uptake kinetics and HbA1c levels in individual red blood cells by Macdonald, Patrick J., Paprocki, Joel D., Xu, Yongjin, Cheng, Alan, Dunn, Tim C., Tetin, Sergey Y.

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Characterization of Fluorescently Labeled Protein with Electrospray Ionization-MS and Fluorescence Spectroscopy: How Random is Random Labeling? by Ruan, Qiaoqiao, Zhao, Cheng, Ramsay, Carol S, Tetin, Sergey Y

“…Solvent exposed lysine residues are abundantly present in many proteins. Their highly reactive ε-amino groups serve as universal targets for coupling with…”
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Accuracy of protein secondary structure determination from circular dichroism spectra based on immunoglobulin examples by Tetin, Sergey Y, Prendergast, Franklyn G, Venyaminov, Sergei Yu

“…Strong contribution of the aromatic amino acid side chain chromophores to the far-UV circular dichroism (CD) spectra substantially distorts a relatively weak…”
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The photostability of the commonly used biotin-4-fluorescein probe by Haack, Richard A., Swift, Kerry M., Ruan, Qiaoqiao, Himmelsbach, Richard J., Tetin, Sergey Y.

“…Biotin-4-fluorescein (B4F) is a commonly used fluorescent probe for studying biotin-(strept)avidin interactions. During a characterization study of an…”
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Measuring antibody affinity and performing immunoassay at the single molecule level by Tetin, Sergey Y., Swift, Kerry M., Matayoshi, Edmund D.

“…Fluorescence correlation spectroscopy (FCS) enables direct observation of the translational diffusion of single fluorescent molecules in solution. When…”
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A rainbow of acridinium chemiluminescence by Best, Quinn A., Haack, Richard A., Swift, Kerry M., Bax, Brian M., Tetin, Sergey Y., Hershberger, Stefan J.

“…Multicolor chemiluminescent acridinium derivatives were synthesized by attaching various common fluorophores to the N10‐acridinium position through a…”
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Multiprobe Spectroscopic Investigation of Molecular-level Behavior within Aqueous 1-Butyl-3-methylimidazolium Tetrafluoroborate by Sarkar, Abhra, Ali, Maroof, Baker, Gary A, Tetin, Sergey Y, Ruan, Qiaoqiao, Pandey, Siddharth

“…In this work, an array of molecular-level solvent featuresincluding solute−solvent/solvent−solvent interactions, dipolarity, heterogeneity, dynamics, probe…”
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Rapid single-molecule imaging in cyclic olefin copolymer channels by Skinner, Joseph P., Tetin, Sergey Y.

“…ABSTRACT Rapid preparation of high quality capture surfaces is a major challenge for surface‐based single‐molecule protein binding assays. Here we introduce a…”
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Affinity assisted selection of antibodies for Point of Care TSH immunoassay with limited wash by Wang, Dan, Skinner, Joseph P., Ruan, Qiaoqiao, Tetin, Sergey Y., Collier, G. Bruce

“…Molecular binding characteristics of several thyroid stimulating hormone (TSH) antibodies were determined for the TSH antigen, along with its closely related…”
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Simplified confocal microscope for counting particles at low concentrations by Skinner, Joseph P, Swift, Kerry M, Ruan, Qiaoqiao, Perfetto, Sergio, Gratton, Enrico, Tetin, Sergey Y

“…We describe a compact scanning confocal fluorescence microscope capable of detecting particles concentrations less than 100 particles∕ml in ~15 min. The system…”
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