An Improved Method of DNA Isolation from Plants Collected in the Field and Conserved in Saturated NaCl/CTAB Solution

A simple method for isolation of genomic DNA from wild plants sampled in remote field areas is presented. The protocol combines NaCl/CTAB leaf preservation with sorbitol extraction of secondary compounds which often contain inhibitors of Taq DNA polymerase activity. The obtained DNA is suitable for...

Full description

Saved in:

Bibliographic Details
Published in:	Taxon Vol. 49; no. 1; pp. 79 - 84
Main Authors:	Štorchová, Helena, Hrdličková, Radmila, Chrtek, Jindřich, Tetera, Martin, Fitze, Dorothee, Fehrer, Judith
Format:	Journal Article
Language:	English
Published:	International Bureau for Plant Taxonomy and Nomenclature 01-02-2000
Subjects:	Genomics Grants Leaves Materials preservation Methods and Techniques Plant products Plants Polymerase chain reaction Silica gel Taxa Wild plants
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A simple method for isolation of genomic DNA from wild plants sampled in remote field areas is presented. The protocol combines NaCl/CTAB leaf preservation with sorbitol extraction of secondary compounds which often contain inhibitors of Taq DNA polymerase activity. The obtained DNA is suitable for random amplified polymorphic DNA (RAPD) analysis of plant populations as well as for specific amplification of chloroplast DNA sequences. The NaCl/CTAB leaf preservation is a powerful alternative to silica gel drying-based preservation.
ISSN:	0040-0262 1996-8175
DOI:	10.2307/1223934