Studies on the Determination of Plasma Renin Activity A method for purification of angiotensin produced
In the previous report, we described on suppression of angiotensinase (ATase) activity, and some ;properties of renin. The present paper concerns to the purification of angiotensin (AT) produced. We tried to extract and to condensate AT in plasma with several organic solvents, ion exchange chromatog...
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| Published in: | The Japanese Journal of Nephrology Vol. 13; no. 6; pp. 725 - 731 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English Japanese |
| Published: |
Japanese Society of Nephrology
1971
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| Online Access: | Get full text |
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| Summary: | In the previous report, we described on suppression of angiotensinase (ATase) activity, and some ;properties of renin. The present paper concerns to the purification of angiotensin (AT) produced. We tried to extract and to condensate AT in plasma with several organic solvents, ion exchange chromatography or evaporation in reduced pressure. Then, we made it a rule to use the combination of n-butanol and water extraction, and ion exchange chromatography. The recovery of added AT was 70 percent (range from 55 to 85%) by this method. Combining this method with the previous report, we decided a method for determination of plasma renin activity. AT concentration in the assaying solution is about 3 fold larger than before the extraction treatment. But this condensation rate is still not enough, as well as the recovery of AT. Neverthealess, we consider that our method is clinically applicable. |
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| ISSN: | 0385-2385 1884-0728 |
| DOI: | 10.14842/jpnjnephrol1959.13.725 |