Tryptophan Attenuates the Effects of OTA on Intestinal Morphology and Local IgA/IgY Production in Broiler Chicks

Tryptophan Attenuates the Effects of OTA on Intestinal Morphology and Local IgA/IgY Production in Broiler Chicks

Ochratoxin A (OTA) is a mycotoxin produced by species of and that can contaminate products of plant origin that are used as animal feed. Through oral exposure, this mycotoxin primarily affects the chicken gastrointestinal system. The present study evaluated the intestinal toxic effects of OTA and th...

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Bibliographic Details
Published in:Toxins Vol. 13; no. 1; p. 5
Main Authors: Ricci, Fernando Galdino, Terkelli, Leticia Rodrigues, Venancio, Emerson José, Justino, Larissa, Dos Santos, Beatriz Queiroz, Baptista, Ana Angelita Sampaio, Oba, Alexandre, de Oliveira Souza, Bianca Dorana, Bracarense, Ana Paula Frederico Rodrigues Loureiro, Hirooka, Elisa Yoko, Itano, Eiko Nakagawa
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 23-12-2020
MDPI
Subjects:
Age
Animal feed
Animals
Antibodies
Body weight
Chickens
Chicks
crypts
Cytokines
Cytology
Defense mechanisms
Enzyme-linked immunosorbent assay
Exposure
Feces
Feeds
Fungi
Gastrointestinal system
Histology
IgA
IgY
Immunoglobulin A
Immunoglobulin Y
Immunoglobulins
Immunohistochemistry
Intestine
Investigations
Juveniles
Morphology
Mucosa
Mycotoxins
Ochratoxin A
Pathogens
Permeability
Poultry
Small intestine
Toxicity
Tryptophan
villi
Villus
IgA
IgY
crypts
mycotoxins
villi
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Summary:Ochratoxin A (OTA) is a mycotoxin produced by species of and that can contaminate products of plant origin that are used as animal feed. Through oral exposure, this mycotoxin primarily affects the chicken gastrointestinal system. The present study evaluated the intestinal toxic effects of OTA and the introduction of L-tryptophan to alleviate these effects in chickens. One-day-old chicks were exposed to a single OTA dose (1.4 mg/kg body weight-b.w.) and treated with or without four daily doses of L-tryptophan (100 mg/kg b.w.). Duodenal villus height/crypt depth, fecal immunoglobulin A/immunoglobulin Y (IgA/IgY) levels, and duodenal positive immunoglobulin A cells (IgA ) were evaluated by histology, ELISA, and immunohistochemistry, respectively, on the 14th day. There were significant changes in the duodenal villus height, crypt depth, and levels of fecal IgA/IgY and duodenal IgA cells ( < 0.05) in groups exposed to OTA. On the other hand, groups exposed to OTA and treated with L-tryptophan showed similar levels of villus height, IgA/IgY levels, and duodenal IgA cells to those of the control group ( > 0.05). In conclusion, exposure to a single dose of OTA orally induces changes in intestinal morphology, levels of IgA/IgY antibodies, and IgA cells. Thus, treatment with L-tryptophan may be a valid alternative means to reduce the harmful effects of OTA on the intestinal mucosa, which requires further study.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins13010005