Changes in the content of poplar isoplastocyanins a and b during vegetation cycle

Changes in the content of poplar isoplastocyanins a and b during vegetation cycle

Changes in the content of isoplastocyanins a (PCa) and b (PCb) in Populus nigra leaves were studied during complete vegetation cycle (from April 30 to October 28). Measurements were made every 7 days. The procedure included extraction and purification of total protein followed by analytical isoelect...

Full description

Saved in:
Bibliographic Details
Published in:Russian journal of plant physiology Vol. 58; no. 1; pp. 181 - 184
Main Authors: Dimitrov, M. I, Donchev, A. A, Shosheva, A. Ch, Getov, V. I, Terezova, N. P, Stoichev, S. D
Format: Journal Article
Language:English
Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 2011
SP MAIK Nauka/Interperiodica
Subjects:
anion-exchange chromatography
Biomedical and Life Sciences
Brief Communications
densitometry
dimorphism
isoelectric focusing
Life Sciences
photosynthesis
Plant Physiology
Plant Sciences
plastocyanin
Populus nigra
photosynthesis
var
isoelectric focusing
densitometry
plastocyanin
dimorphism
anion-exchange chromatography
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Changes in the content of isoplastocyanins a (PCa) and b (PCb) in Populus nigra leaves were studied during complete vegetation cycle (from April 30 to October 28). Measurements were made every 7 days. The procedure included extraction and purification of total protein followed by analytical isoelectric focusing and densitometry of obtained isophoregrams. The positions of PCa and PCb were determined by comparison with the positions of the highly purified two isoforms. The PCb/PCa ratio was calculated from the areas of the corresponding densitometric peaks. The amount of PCb was one half of PCa amount in the earliest stage of the leaf formation as well as during the longer part of the summer, e.g., the second half of July, August, and September. During the most active period of the poplar growth, e.g., June and the first half of July, the ratio was about or above unity. This result could be important for understanding the specific functional significance of PCa and PCb.
Bibliography:http://dx.doi.org/10.1134/S1021443711010055
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443711010055