Simultaneous quantification of arctigenin and its glucuronide conjugate in mouse plasma using ultra‐high performance liquid chromatography coupled to tandem mass spectrometry

Arctigenin is a natural lignin and a main active component of Fructus arctii, the dried fruit of Arctium lappa. This compound was reported to have some biological activities such as anti‐inflammatory, antioxidant, antiviral, renoprotective, and antitumor effects. Arctigenin is mainly metabolized to...

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Bibliographic Details
Published in:	Journal of separation science Vol. 44; no. 7; pp. 1299 - 1306
Main Authors:	Suzuki, Yosuke, Sato, Michiko, Awazuhara, Takuya, Nukui, Yusuke, Yoshida, Airi, Terashima, Tomoka, Watanabe, Keita, Fujioka, Rumi, Tsuchihara, Katsuya, Kishino, Satoshi, Ohno, Keiko
Format:	Journal Article
Language:	English
Published:	Germany Wiley Subscription Services, Inc 01-04-2021
Subjects:	Anticancer properties Antioxidants arctigenin arctigenin‐4′‐O‐glucuronide Chromatography High performance liquid chromatography Mass spectrometry Plasma Scientific imaging Spectroscopy arctigenin-4′-O-glucuronide mass spectrometry arctigenin plasma
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Summary:	Arctigenin is a natural lignin and a main active component of Fructus arctii, the dried fruit of Arctium lappa. This compound was reported to have some biological activities such as anti‐inflammatory, antioxidant, antiviral, renoprotective, and antitumor effects. Arctigenin is mainly metabolized to arctigenin‐4′‐O‐glucuronide by UDP‐glucuronosyltransferase. In this study, a simultaneous quantification method was established and validated for measuring arctigenin and arctigenin‐4′‐O‐glucuronide in mouse plasma using ultra‐high performance liquid chromatography with tandem mass spectrometry. The assay fulfilled the requirements of the United States Food and Drug Administration guideline for assay validation, with a lower limit of quantification of 2.00 ng/mL for arctigenin and 50.0 ng/mL for arctigenin‐4′‐O‐glucuronide. The recovery rate and matrix effect ranged from 78.4 to 102.8% and 92.5 to 106.3%, respectively, for arctigenin, and 74.3 to 109.2% and 94.9 to 110.2% for arctigenin‐4′‐O‐glucuronide. The method was applied to the measurement of plasma concentrations of arctigenin and arctigenin‐4′‐O‐glucuronide in the plasma of mice after administration of arctigenin. All measured concentrations were within the calibration ranges. Our novel method may be useful to measure plasma arctigenin and arctigenin‐4′‐O‐glucuronide concentrations, and contribute to evaluate the pharmacokinetics of arctigenin and arctigenin‐4′‐O‐glucuronide in mice.
ISSN:	1615-9306 1615-9314
DOI:	10.1002/jssc.202001078