Soy protein diet, but not Lactobacillus rhamnosus GG, decreases mucin-1, trefoil factor-3, and tumor necrosis factor-α in colon of dextran sodium sulfate-treated C57BL/6 mice

The incidence of inflammatory bowel diseases has increased during recent decades. Within the colon, the families of mucins (MUC) and trefoil factors (TFF) facilitate mucosal protection. Probiotic administration influences the intestinal MUC layer. Additionally, food components may affect gut microfl...

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Bibliographic Details
Published in:	The Journal of nutrition Vol. 141; no. 7; p. 1239
Main Authors:	Jiang, Huanyi, Przybyszewski, Joseph, Mitra, Debjani, Becker, Chad, Brehm-Stecher, Byron, Tentinger, Amy, MacDonald, Ruth S
Format:	Journal Article
Language:	English
Published:	United States 01-07-2011
Subjects:	Animals Base Sequence Caseins - administration & dosage Cecum - metabolism Cecum - microbiology Colitis, Ulcerative - chemically induced Colitis, Ulcerative - diet therapy Colitis, Ulcerative - genetics Colitis, Ulcerative - therapy Colon - metabolism Colon - microbiology Dextran Sulfate - toxicity Disease Models, Animal Female Gene Expression Lacticaseibacillus rhamnosus Mice Mice, Inbred C57BL Milk Proteins - administration & dosage Mucin-1 - genetics Mucins - genetics Probiotics - administration & dosage RNA, Messenger - genetics RNA, Messenger - metabolism Soybean Proteins - administration & dosage Trefoil Factor-3 Tumor Necrosis Factor-alpha - genetics Whey Proteins
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Summary:	The incidence of inflammatory bowel diseases has increased during recent decades. Within the colon, the families of mucins (MUC) and trefoil factors (TFF) facilitate mucosal protection. Probiotic administration influences the intestinal MUC layer. Additionally, food components may affect gut microflora or have direct effects on the MUC barrier. Our objective was to determine whether diet and/or Lactobacillus rhamnosus GG (LGG) would mediate dextran sodium sulfate (DSS)-induced colitis by altering expression of the MUC and TFF genes. C57BL/6 mice were fed diets containing 20% (wt:wt) casein, soy, or whey proteins with or without LGG for 12 d. Seven days after starting LGG diets, the mice were given 2% DSS in drinking water for 4 d. Two additional casein groups with or without LGG were given tap water, for a total of 8 groups. One day after the DSS treatment, the mice were killed and the colon and cecum tissues and cecum contents were collected and analyzed by qRT-PCR. Whey protein significantly increased cecal LGG content compared with the other diets. In the casein diet groups, MUC1 and TFF-3 expression in colon was significantly induced by DSS independent of LGG. Compared with other DSS-treated groups, soy protein decreased MUC-1 and TFF-3 in the colon. Similarly, soy protein decreased the impact of DSS on inflammatory scores, TNFα gene expression, and colon shortening. There was no overall effect of LGG on these measurements. In conclusion, soy protein suppressed the DSS-induced inflammatory stimulation of MUC, TFF, and TNFα gene expression independently of LGG.
ISSN:	1541-6100
DOI:	10.3945/jn.110.137414