Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF

Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF

Bone-substitute materials are essential in dental implantology. We prepared collagen (Col)/hydroxyapatite (Hap)/acidic gelatin (AG)/basic fibroblast growth factor (b-FGF) constructs with enhanced bone-forming capability. The Col/Hap apatite composites were prepared by immersing Col sponges alternate...

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Bibliographic Details
Published in:Materials Vol. 15; no. 24; p. 8802
Main Authors: Hoshi, Miki, Taira, Masayuki, Sawada, Tomofumi, Hachinohe, Yuki, Hatakeyama, Wataru, Takafuji, Kyoko, Tekemoto, Shinji, Kondo, Hisatomo
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 01-12-2022
MDPI
Subjects:
acidic-gelatin
alternate immersion method
Analysis
Apatite
basic-fibroblast growth factor
Biological products
Biomedical materials
bone formation
Bones
Calcium phosphates
Collagen
collagen/hydroxyapatite composite
Composite materials
Crystal defects
Diffraction
Fibroblast growth factors
Fibroblasts
Fourier transforms
Gelatin
Growth factors
Hydroxyapatite
hydroxyapatite precipitation
Infrared analysis
Infrared spectroscopy
Methods
Precipitates
Skin
Soft x rays
Sponges
Submerging
Tissue engineering
X-ray spectroscopy
X-rays
Japan
United States > US
alternate immersion method
basic-fibroblast growth factor
collagen/hydroxyapatite composite
hydroxyapatite precipitation
bone formation
bone histology
bone substitute materials
acidic-gelatin
dental implantology
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Summary:Bone-substitute materials are essential in dental implantology. We prepared collagen (Col)/hydroxyapatite (Hap)/acidic gelatin (AG)/basic fibroblast growth factor (b-FGF) constructs with enhanced bone-forming capability. The Col/Hap apatite composites were prepared by immersing Col sponges alternately in calcium and phosphate ion solutions five times, for 20 and 60 min, respectively. Then, the sponges were heated to 56 °C for 48 h. Scanning electron microscopy/energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction analyses showed that the Col/Hap composites contained poorly crystalline Hap precipitates on the Col matrix. Col/Hap composite granules were infiltrated by AG, freeze-dried, and immersed in b-FGF solution. The wet quaternary constructs were implanted in rat cranial bone defects for 8 weeks, followed by soft X-ray measurements and histological analysis. Animal studies have shown that the constructs moderately increase bone formation in cranial bone defects. We found that an alternate immersion time of 20 min led to the greatest bone formation (p < 0.05). Constructs placed inside defects slightly extend the preexisting bone from the defect edges and lead to the formation of small island-like bones inside the defect, followed by disappearance of the constructs. The combined use of Col, Hap, AG, and b-FGF might bring about novel bone-forming biomaterials.
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ISSN:1996-1944
1996-1944
DOI:10.3390/ma15248802