Crude extract of Jatobá leaves promotes canine osteosarcoma cell D17 proliferation

New substances for neoplasm treatment have to be carefully studied to minimize adverse effects and prevent disease progression stimulation. Jatobá is a typical tree of the and biome, with antifungal, antimicrobial, larvicide, antioxidant, and antiproliferative properties. This study aimed to investi...

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Bibliographic Details
Published in:	Veterinary World Vol. 15; no. 5; pp. 1283 - 1289
Main Authors:	Vieira, V S, Cruz, V S, Nepomuceno, L L, Soares, N P, Arnhold, E, Teixeira, W F P, Vieira, D S, Borges, J C A, Paixão, F M, Araújo, E G
Format:	Journal Article
Language:	English
Published:	India Veterinary World 01-05-2022
Subjects:	antineoplastic chemotherapy biodiversity bone tumors hymenaea martiana hayne medicinal plants bone tumors Hymenaea martiana Hayne biodiversity medicinal plants antineoplastic chemotherapy
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Summary:	New substances for neoplasm treatment have to be carefully studied to minimize adverse effects and prevent disease progression stimulation. Jatobá is a typical tree of the and biome, with antifungal, antimicrobial, larvicide, antioxidant, and antiproliferative properties. This study aimed to investigate the action of the crude extract of Jatobá leaves (EBFJ) on canine osteosarcoma (CO) cells and analyze the expression of biomarkers in neoplasm progression. D17 cells were cultured and subjected to treatment with EBFJ at different concentrations (10 μg/mL; 100 μg/mL; 1000 μg/mL; 2000 μg/mL; and 5000 μg/mL) and exposure times (24 h, 48 h, and 72 h). The tetrazolium reduction assay and the immunocytochemistry technique, with anti-Bcl2, anti-p53, and anti-Ki-67 antibodies, were used to observe the effect of the extract on cell proliferation. Doses of 2000 µg and 5000 µg had cell viability of 300.80% and 361.84%, respectively. The extract did not show significant cytotoxicity of samples with the control group. The confluence of cells, the number of labeled cells, and the expression of Bcl2, Ki-67, and p53 were higher in the groups treated with EBFJ, with a statistical difference from the group without treatment. EBFJ was not cytotoxic and had a proliferative effect on CO D17 cells. The confluence of cells, the number of labeled cells, and the expression of Bcl2, Ki-67, and p53 were higher in the groups treated with the extract.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0972-8988 2231-0916
DOI:	10.14202/vetworld.2022.1283-1289