The Operculina macrocarpa (l.) urb. (jalapa) tincture modulates human blood platelet aggregation

Operculina macrocarpa is an ornamental climbing plant of the Northeastern Brazil extensively used in traditional medicine as depurative of the blood and for the treatment of thrombosis. To investigate the antiplatelet and anticoagulant potential of Operculina macrocarpa and to determine the possible...

Full description

Saved in:

Bibliographic Details
Published in:	Journal of ethnopharmacology Vol. 151; no. 1; pp. 151 - 157
Main Authors:	Pierdoná, Taiana Magalhães, Lima, Nathalia Rocha, Rodrigues, Raony Cássio Millet, Teixeira, Jonas Pires, Gonçalves, Romélia Pinheiro, Fontenele, Juvenia Bezerra, Vasconcelos, Silvânia Maria Mendes, de Barros Viana, Glauce Socorro, Leal, Luzia Kalyne Almeida Moreira
Format:	Journal Article
Language:	English
Published:	Ireland Elsevier Ireland Ltd 10-01-2014
Subjects:	Animals Anticoagulant Antiplatelet Blood Platelets - drug effects Convolvulaceae Convolvulaceae - chemistry Humans Male Medicine, Traditional Mice Operculina macrocarpa Plant Extracts - chemistry Plant Extracts - pharmacology Platelet Aggregation - drug effects Platelet Aggregation Inhibitors - chemistry Platelet Aggregation Inhibitors - pharmacology Rats Operculina macrocarpa Antiplatelet Anticoagulant Convolvulaceae
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Operculina macrocarpa is an ornamental climbing plant of the Northeastern Brazil extensively used in traditional medicine as depurative of the blood and for the treatment of thrombosis. To investigate the antiplatelet and anticoagulant potential of Operculina macrocarpa and to determine the possible mechanisms of action. The Operculina macrocarpa tincture (OMT) was characterized by the polyphenol content and chromatographic profile established by HPLC with detection and quantification of three phenol acids (caffeic, clorogenic and gallic acids). The human platelet aggregation was induced in vitro by the agonists ADP, collagen, thrombin, epinephrine or arachidonic acid, and the antiplatelet effect of OMT was evaluated in the presence or absence of aspirin (a nonselective inhibitor of cyclooxygenase), pentoxifylline (a phosphodiesterase inhibitor), ticlopidine (a P2Y12 purinoceptor antagonist) or ODQ (a selective inhibitor of guanilate cyclase). The effect of OMT on the partial thromboplastin time, prothrombin time and bleeding time were investigated on human or rat plasma. The strongest antiplatelet effect of OMT (50–400µg/mL) was observed on the ADP- induced aggregation with inhibitions up to 55%, while among others agonists (epinephrine, collagen, thrombin and arachidonic acid) maximal inhibitions reached by OMT (200µg/mL) were on platelet aggregation induced by collagen (18%) or epinephrine (20%). The antiplatelet effect of OMT (400µg/mL) was comparable to aspirin, a nonspecific inhibitor of cyclooxygenase. The ticlopidine and pentoxifylline increased 5.1 and 3.8 fold the inhibitory effect of OMT on ADP-induced platelet aggregation, respectively. On the other hand, l-arginine, ODQ and aspirin showed a slightly or no effect on antiplatelet effect of OMT. The bleeding time in rats was significantly increased by OMT, but the tincture did not interfere on the activated partial thromboplastin or prothrombin time in human plasma. This study showed that the tincture of Operculina macrocarpa has antiplatelet effect that cannot be attributed to a single biochemical mechanism and at least part of it cannot be related to the OMT inhibition of P2Y12 purinergic receptors. [Display omitted] Original record of the antiplatelet effect of standard Operculina macrocarpa tincture (HPLC-PDA) on ADP-induced platelet aggregation in human plasma.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-8741 1872-7573
DOI:	10.1016/j.jep.2013.10.008