Search Results - "Taylor, Benjamin J M"

CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs by Coelho, Matthew A., De Braekeleer, Etienne, Firth, Mike, Bista, Michal, Lukasiak, Sebastian, Cuomo, Maria Emanuela, Taylor, Benjamin J. M.

“…Precise genome editing using CRISPR-Cas9 is a promising therapeutic avenue for genetic diseases, although off-target editing remains a significant safety…”
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BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B by Coelho, Matthew A, Li, Songyuan, Pane, Luna Simona, Firth, Mike, Ciotta, Giovanni, Wrigley, Jonathan D, Cuomo, Maria Emanuela, Maresca, Marcello, Taylor, Benjamin J M

“…Base Editing is a precise genome editing method that uses a deaminase-Cas9 fusion protein to mutate cytidine to thymidine in target DNA in situ without the…”
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Author Correction: Universal toxin-based selection for precise genome engineering in human cells by Li, Songyuan, Akrap, Nina, Cerboni, Silvia, Porritt, Michelle J., Wimberger, Sandra, Lundin, Anders, Möller, Carl, Firth, Mike, Gordon, Euan, Lazovic, Bojana, Sieńska, Aleksandra, Pane, Luna Simona, Coelho, Matthew A., Ciotta, Giovanni, Pellegrini, Giovanni, Sini, Marcella, Xu, Xiufeng, Mitra, Suman, Bohlooly-Y, Mohammad, Taylor, Benjamin J. M., Sienski, Grzegorz, Maresca, Marcello

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Decoding non-random mutational signatures at Cas9 targeted sites by Taheri-Ghahfarokhi, Amir, Taylor, Benjamin J M, Nitsch, Roberto, Lundin, Anders, Cavallo, Anna-Lina, Madeyski-Bengtson, Katja, Karlsson, Fredrik, Clausen, Maryam, Hicks, Ryan, Mayr, Lorenz M, Bohlooly-Y, Mohammad, Maresca, Marcello

“…Abstract The mutation patterns at Cas9 targeted sites contain unique information regarding the nuclease activity and repair mechanisms in mammalian cells…”
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Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease by Rueda, Fernando Orden, Bista, Michal, Newton, Matthew D., Goeppert, Anne U., Cuomo, M. Emanuela, Gordon, Euan, Kröner, Felix, Read, Jon A., Wrigley, Jonathan D., Rueda, David, Taylor, Benjamin J. M.

“…The CRISPR–Cas9 RNA-guided endonuclease system allows precise and efficient modification of complex genomes and is continuously developed to enhance…”
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Universal toxin-based selection for precise genome engineering in human cells by Li, Songyuan, Akrap, Nina, Cerboni, Silvia, Porritt, Michelle J., Wimberger, Sandra, Lundin, Anders, Möller, Carl, Firth, Mike, Gordon, Euan, Lazovic, Bojana, Sieńska, Aleksandra, Pane, Luna Simona, Coelho, Matthew A., Ciotta, Giovanni, Pellegrini, Giovanni, Sini, Marcella, Xu, Xiufeng, Mitra, Suman, Bohlooly-Y, Mohammad, Taylor, Benjamin J. M., Sienski, Grzegorz, Maresca, Marcello

“…Prokaryotic restriction enzymes, recombinases and Cas proteins are powerful DNA engineering and genome editing tools. However, in many primary cell types, the…”
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Systematic Evaluation of CRISPRa and CRISPRi Modalities Enables Development of a Multiplexed, Orthogonal Gene Activation and Repression System by Martella, Andrea, Firth, Mike, Taylor, Benjamin J. M, Göppert, Anne, Cuomo, Emanuela M, Roth, Robert G, Dickson, Alan J, Fisher, David I

“…The ability to manipulate the expression of mammalian genes using synthetic transcription factors is highly desirable in both fields of basic research and…”
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Active RNAP pre-initiation sites are highly mutated by cytidine deaminases in yeast, with AID targeting small RNA genes by Taylor, Benjamin J M, Wu, Yee Ling, Rada, Cristina

“…Cytidine deaminases are single stranded DNA mutators diversifying antibodies and restricting viral infection. Improper access to the genome leads to…”
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DNA deaminases induce break-associated mutation showers with implication of APOBEC3B and 3A in breast cancer kataegis by Taylor, Benjamin Jm, Nik-Zainal, Serena, Wu, Yee Ling, Stebbings, Lucy A, Raine, Keiran, Campbell, Peter J, Rada, Cristina, Stratton, Michael R, Neuberger, Michael S

“…Breast cancer genomes have revealed a novel form of mutation showers (kataegis) in which multiple same-strand substitutions at C:G pairs spaced one to several…”
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