Treatment Outcomes of Alginate-Embedded Allogenic Mesenchymal Stem Cells Versus Autologous Chondrocytes for the Repair of Focal Articular Cartilage Defects in a Rabbit Model

Background: Mesenchymal stem cells (MSCs) represent a promising alternative form of cell-based therapy for cartilage injury. However, the capacity of MSCs for chondrogenesis has not been fully explored. In particular, there is presently a lack of studies comparing the effectiveness of MSCs to conven...

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Published in:	The American journal of sports medicine Vol. 40; no. 1; pp. 83 - 90
Main Authors:	Tay, Liang Xin, Ahmad, Raja Elina, Dashtdar, Havva, Tay, K.W., Masjuddin, T., Ab-Rahim, S., Chong, Pan Pan, Selvaratnam, L., Kamarul, T.
Format:	Journal Article
Language:	English
Published:	Los Angeles, CA SAGE Publications 01-01-2012 Sage Publications Sage Publications Ltd
Subjects:	Alginates - pharmacology Animals Biological and medical sciences Cartilage Cartilage, Articular - injuries Cartilage, Articular - surgery Cell differentiation, maturation, development, hematopoiesis Cell physiology Cells, Cultured Chondrocytes - transplantation Disease Models, Animal Diseases of the osteoarticular system Fundamental and applied biological sciences. Psychology Glycosaminoglycans - metabolism Immunoenzyme Techniques Knee Knee Injuries - surgery Male Medical sciences Mesenchymal Stem Cell Transplantation - methods Molecular and cellular biology Rabbits Random Allocation Sports medicine Stem cells Therapy Transplantation, Autologous Transplantation, Homologous Wound Healing chondrogenic differentiation mesenchymal stem cell chondrocyte cartilage repair Prognosis Chondrocyte Stem cell Rabbit Alginates Mesenchymal cell Lagomorpha Cell differentiation Vertebrata Autograft Mammalia Treatment Articular cartilage Ergonomics Animal Graft Models Repair Comparative study
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Summary:	Background: Mesenchymal stem cells (MSCs) represent a promising alternative form of cell-based therapy for cartilage injury. However, the capacity of MSCs for chondrogenesis has not been fully explored. In particular, there is presently a lack of studies comparing the effectiveness of MSCs to conventional autologous chondrocyte (autoC) treatment for regeneration of full-thickness cartilage defects in vivo. Hypothesis: Treatment with allogenic undifferentiated MSCs (alloMSCs) results in superior cartilage tissue regeneration profiles when compared with autoC for repair of focal articular cartilage defects. Study Design: Controlled laboratory study. Methods: Full-thickness articular cartilage defects were created on the weightbearing surface of the medial femoral condyles in both knees of New Zealand White rabbits (N = 30). Six weeks after the defect was induced, the right knee was treated with either alloMSCs (n = 12) or autoC (n = 18), while the left knee remained untreated (control). The rabbits were sacrificed at 6 months after treatment for assessment of cartilage tissue regeneration, which included the Brittberg morphologic score, histologic grading by O’Driscoll score, and quantitative analysis of glycosaminoglycans per total protein content. Results: Apart from significantly higher Brittberg scores in the alloMSC treatment group (8.8 ± 0.8) versus the autoC treatment group (6.6 ± 0.8) (P = .04), both treatments showed similar cartilage regenerative profiles. All outcome measures were significantly higher in the treatment groups compared with their respective controls (P < .05). Conclusion: AlloMSCs have similar effectiveness as autoC for repair of focal cartilage defects. Both treatments resulted in superior tissue regeneration compared with untreated defects. Clinical Relevance: The results have an implication of supporting the potential use of MSCs for cartilage repair after sports injuries or diseases, in view of similar efficacy but less patient morbidity and potential cost savings as compared with conventional autoC therapy.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0363-5465 1552-3365
DOI:	10.1177/0363546511420819