Identification of stress-responsive genes in Caenorhabditis elegans using RT-PCR differential display

In order to identify genes that are differentially expressed as a consequence of oxidative stress due to paraquat we used the differential display technique to compare mRNA expression patterns in Caenorhabditis elegans. A C.elegans mixed stage worm population and a homogeneous larval population were...

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Bibliographic Details
Published in:	Nucleic acids research Vol. 26; no. 7; pp. 1621 - 1627
Main Authors:	Tawe, Wilson N., Eschbach, Marie-Luise, Walter, Rolf D., Henkle-Dührsen, Kimberly
Format:	Journal Article
Language:	English
Published:	England Oxford University Press 01-04-1998
Subjects:	Amino Acid Sequence Animals Caenorhabditis elegans - drug effects Caenorhabditis elegans - genetics Caenorhabditis elegans - physiology DNA, Complementary Gene Expression Regulation - drug effects Genes, Helminth Glutathione Transferase - biosynthesis Larva Leucine Zippers Molecular Sequence Data Paraquat - pharmacology Polymerase Chain Reaction - methods RNA, Helminth - biosynthesis RNA, Messenger - biosynthesis Stress, Physiological Superoxide Dismutase - biosynthesis Transcription Factors - biosynthesis Transcription Factors - chemistry Transcription, Genetic Zinc Fingers
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Summary:	In order to identify genes that are differentially expressed as a consequence of oxidative stress due to paraquat we used the differential display technique to compare mRNA expression patterns in Caenorhabditis elegans. A C.elegans mixed stage worm population and a homogeneous larval population were treated with 100 mM paraquat, in parallel with controls. Induction of four cDNA fragments, designated L-1, M-47, M-96 and M-132, was confirmed by Northern blot analysis with RNA from stressed and unstressed worm populations. A 40-fold increase in the steady-state mRNA level in the larval population was observed for the L-1/M-47 gene, which encodes the detoxification enzyme glutathione S-transferase. A potential stress-responsive transcription factor (M-132) with C2H2-type zinc finger motifs and an N-terminal leucine zipper domain was identified. The M-96 gene encodes a novel stressresponsive protein. Since paraquat is known to generate superoxide radicals in vivo, the response of the C.elegans superoxide dismutase (SOD) genes to paraquat was also investigated in this study. The steady-state mRNA levels of the manganese-type and the copper/zinc-type SODs increased 2-fold in the larval population in response to paraquat, whereas mixed stage populations did not show any apparent increase in the levels of these SOD mRNAs.
Bibliography:	ark:/67375/HXZ-0XTHMQZM-M istex:9C34A5ABB3F97AC6E897661CAA2FF50FE1AC632C ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/26.7.1621