A humanised tissue‐engineered bone model allows species‐specific breast cancer‐related bone metastasis in vivo

A humanised tissue‐engineered bone model allows species‐specific breast cancer‐related bone metastasis in vivo

Bone metastases frequently occur in the advanced stages of breast cancer. At this stage, the disease is deemed incurable. To date, the mechanisms of breast cancer‐related metastasis to bone are poorly understood. This may be attributed to the lack of appropriate animal models to investigate the comp...

Full description

Saved in:
Bibliographic Details
Published in:Journal of tissue engineering and regenerative medicine Vol. 12; no. 2; pp. 494 - 504
Main Authors: Quent, VMC, Taubenberger, AV, Reichert, JC, Martine, LC, Clements, JA, Hutmacher, DW, Loessner, D
Format: Journal Article
Language:English
Published: England Hindawi Limited 01-02-2018
Subjects:
Animal models
Biocompatibility
Biomedical materials
Bone cancer
bone colonisation
Bone growth
bone tissue engineering
Breast cancer
Calcium phosphates
Cancer
Colonization
Fused deposition modeling
fused deposition modelling
humanised animal model
Hydrogels
Integration
Metastases
Metastasis
Osteoclasts
Osteogenesis
Polycaprolactone
polycaprolactone scaffolds
Polyethylene glycol
Regenerative medicine
solution electrospinning
Surgical implants
Tissue engineering
Tricalcium phosphate
polycaprolactone scaffolds
bone colonisation
bone tissue engineering
fused deposition modelling
breast cancer
solution electrospinning
humanised animal model
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bone metastases frequently occur in the advanced stages of breast cancer. At this stage, the disease is deemed incurable. To date, the mechanisms of breast cancer‐related metastasis to bone are poorly understood. This may be attributed to the lack of appropriate animal models to investigate the complex cancer cell–bone interactions. In this study, two established tissue‐engineered bone constructs (TEBCs) were applied to a breast cancer‐related metastasis model. A cylindrical medical‐grade polycaprolactone‐tricalcium phosphate scaffold produced by fused deposition modelling (scaffold 1) was compared with a tubular calcium phosphate‐coated polycaprolactone scaffold fabricated by solution electrospinning (scaffold 2) for their potential to generate ectopic humanised bone in NOD/SCID mice. While scaffold 1 was found not suitable to generate a sufficient amount of ectopic bone tissue due to poor ectopic integration, scaffold 2 showed excellent integration into the host tissue, leading to bone formation. To mimic breast cancer cell colonisation to the bone, MDA‐MB‐231, SUM1315, and MDA‐MB‐231BO breast cancer cells were cultured in polyethylene glycol‐based hydrogels and implanted adjacent to the TEBCs. Histological analysis indicated that the breast cancer cells induced an osteoclastic reaction in the TEBCs, demonstrating analogies to breast cancer‐related bone metastasis seen in patients.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1932-6254
1932-7005
DOI:10.1002/term.2517