Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates

Characterization of an endo‐beta‐1,4 glucanase gene from paper‐degrading and denim bio‐stoning cellulase producing Aspergillus isolates

Cellulases are used in textile, pulp and paper, brewery and wine, sugars, and ethanol industries. Four fungal isolates obtained from organic municipal solid wastes (OMSW) were selected based on their cellulolytic activity on carboxymethyl cellulose (CMC) agar medium. Based on the internal transcribe...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and applied biochemistry Vol. 70; no. 3; pp. 1057 - 1071
Main Authors: Ahmed, Jahed, Taslim, Asma‐Ul‐, Raihan, Topu, Sohag, Md. Mehadi Hasan, Hasan, Mahmudul, Suhani, Sabrina, Qadri, Firdausi, Azad, Abul Kalam
Format: Journal Article
Language:English
Published: United States Wiley Subscription Services, Inc 01-06-2023
Subjects:
Aspergillus
Aspergillus flavus
bio‐stoning
Calcium ions
Carboxymethyl cellulose
Carboxymethylcellulose
Cellulase
Cellulose
CMCase
Ethanol
Fermentation
filter paper
Fungi
Genes
Genomes
internal transcribed spacer
Magnesium
Municipal solid waste
Municipal waste management
Nucleotide sequence
organic municipal solid wastes
Organic wastes
pH effects
Phylogeny
Ribosomal DNA
Solid waste management
Solid wastes
Sugar
Wines
Zinc
organic municipal solid wastes
CMCase
bio-stoning
cellulase
filter paper
internal transcribed spacer
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cellulases are used in textile, pulp and paper, brewery and wine, sugars, and ethanol industries. Four fungal isolates obtained from organic municipal solid wastes (OMSW) were selected based on their cellulolytic activity on carboxymethyl cellulose (CMC) agar medium. Based on the internal transcribed spacer (ITS) sequence of the ribosomal DNA, the four cellulolytic isolates were identified as Aspergillus fumigatus AKAL1, Aspergillus oryzae AKAL4, Aspergillus flavus AKAL8, and Aspergillus flavus AKAL9. After 9 days of fermentation at 30°C and pH 6.5 under 110 rpm agitation, these isolates produced the maximum amount of cellulase. The cellulase showed optimum activity at temperature 35–40°C and pH 6.0–7.0 and was stable for 1 h at 25–45°C and pH 5.0–7.0. The Mg2+ and Zn2+ significantly increased but Hg2+, K+, and Ca2+ severely repressed the cellulase activity. Degradation of filter papers and bio‐stoning of denim was successfully done with the crude cellulase. An endo‐β‐1,4‐glucanase was isolated and characterized from Aspergillus isolates. Genome‐wide analysis revealed that the genomes of A. oryzae, A. fumigatus, and A. flavus, the pertinent species of the fungal isolates, had 23, 25, and 22 cellulase genes, respectively. Phylogenetic analysis revealed that the cellulases in these fungal species were divided into three major groups, and the isolated endo‐β‐1,4‐glucanase clustered to Group II. Ten different motifs are present in cellulases of the three species. Results herein provide a valuable resource for understanding cellulase genes in Aspergillus species and potential application of cellulase in textile and fermentable sugars production industries.
Bibliography:Present Address
Topu Raihan, The United Graduate School of Agricultural Sciences, Tottori University, Koyama‐Minami, Tottori 680–0954, Japan
Mahmudul Hasan, Department of Pharmaceutical and Industrial Biotechnology, Sylhet Agricultural University, Sylhet, Bangladesh
Jahed Ahmed, Laboratoire de Biogenèse Membranaire ‐ UMR 5200 ‐ CNRS, Université de Bordeaux, Villenave‐d'Ornon, France
Associate Editor: Francesca Valetti
Md. Mehadi Hasan Sohag, Department of Genetic Engineering and Biotechnology, Jagannath University, Dhaka, Bangladesh
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.2420