Changes in PACAP Immunoreactivity in Human Milk and Presence of PAC1 Receptor in Mammary Gland during Lactation

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence in the nervous system and peripheral organs, including the mammary gland. Previously, we have shown that PACAP38 is present in the human milk at higher levels than in respective blood samples. How...

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Bibliographic Details
Published in:	Journal of molecular neuroscience Vol. 48; no. 3; pp. 631 - 637
Main Authors:	Csanaky, Katalin, Banki, Eszter, Szabadfi, Krisztina, Reglodi, Dora, Tarcai, Ibolya, Czegledi, Levente, Helyes, Zsuzsanna, Ertl, Tibor, Gyarmati, Judit, Szanto, Zalan, Zapf, Istvan, Sipos, Erika, Shioda, Seiji, Tamas, Andrea
Format:	Journal Article
Language:	English
Published:	New York Humana Press Inc 01-11-2012 Springer Nature B.V
Subjects:	Animals Biomedical and Life Sciences Biomedicine Breast - chemistry Breast - physiology Breast cancer Breast milk Cell Biology Colostrum Colostrum - chemistry Female Gene Expression Regulation, Developmental Growth factors Hormones Humans Immunoreactivity Lactation Lactation - physiology Mammary gland Mammary Glands, Animal - chemistry Mammary Glands, Animal - physiology Milk, Human - chemistry Neonates Nervous system Neurochemistry Neurology Neuropeptides Neurosciences PAC1 protein Pituitary adenylate cyclase-activating polypeptide Pituitary Adenylate Cyclase-Activating Polypeptide - analysis Plasma Polypeptides Proteomics Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - analysis Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - biosynthesis Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I - genetics Sheep Sheep, Domestic - physiology Signal transduction Species Specificity Hungary Human milk PACAP38 PAC1 Mammary gland
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Summary:	Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuropeptide with widespread occurrence in the nervous system and peripheral organs, including the mammary gland. Previously, we have shown that PACAP38 is present in the human milk at higher levels than in respective blood samples. However, it is not known how PACAP levels and the expression of PAC1 receptor change during lactation. Therefore, the aim of our study was to investigate PACAP38-like immunoreactivity (PACAP38-LI) in human colostrums and transitional and mature milk during lactation and to compare the expression of PAC1 receptors in lactating and non-lactating mammary glands. We found that PACAP38-LI was significantly higher in human colostrum samples than in the transitional and mature milk. PACAP38-LI did not show any significant changes within the first 10-month period of lactation, but a significant increase was observed thereafter, up to the examined 17th month. Weak expression of PAC1 receptors was detected in non-lactating sheep and human mammary glands, but a significant increase was observed in the lactating sheep samples. In summary, the present study is the first to show changes of PACAP levels in human milk during lactation. The presence of PACAP in the milk suggests a potential role in the development of newborn, while the increased expressions of PAC1 receptors on lactating breast may indicate a PACAP38/PAC1 interaction in the mammary gland during lactation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0895-8696 1559-1166
DOI:	10.1007/s12031-012-9779-4