Identification of stylar RNases associated with gametophytic self-incompatibility in almond (Prunus dulcis)

Stylar proteins of 13 almond (Prunus dulcia) cultivars with known S-genotypes were surveyed by IEF and 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-RNase associated with gametophytic self-incompatibility (SI) in this plant species. RNase activities corre...

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Bibliographic Details
Published in:	Plant and cell physiology Vol. 38; no. 3; pp. 304 - 311
Main Authors:	Tao, R. (Kyoto Univ. (Japan). Coll. of Agriculture), Yamane, H, Sassa, H, Mori, H, Gradziel, T.M, Dandekar, A.M, Sugiura, A
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-03-1997
Subjects:	Agronomy. Soil science and plant productions Almond Amino Acid Sequence AUTOESTERILIDAD AUTOSTERILITE Biological and medical sciences DETERIORATION DETERIORO Fundamental and applied biological sciences. Psychology GAMETE GAMETES GAMETOS Gasmetophytic self incompatibitity Genetics and breeding of economic plants Genotype GINECEO Glycoproteins - analysis GYNECEE GYNOECIUM Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia IDENTIFICACION IDENTIFICATION ISoelectric focusing Molecular Sequence Data Nuts - enzymology Nuts - genetics Peptide Fragments Plant breeding: fundamental aspects and methodology Plant physiology and development Plant Proteins - analysis Plant Proteins - chemistry PRUNUS AMYGDALUS Prunus dulcis RIBONUCLEASAS RIBONUCLEASE RIBONUCLEASES Ribonucleases - analysis Ribonucleases - chemistry S-RNase SELF STERILITY Sequence Homology, Amino Acid Sexual reproduction Two dimensional gel electrophoresis Vegetative and sexual reproduction, floral biology, fructification Enzyme Prunus dulcis Rosaceae Esterases Ribonuclease Sexual reproduction Gynoecium Immunological method Characterization Proteins Selfincompatibility Dicotyledones Angiospermae Hydrolases Spermatophyta Fruit tree Comparative study Aminoacid sequence Cultivar
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Summary:	Stylar proteins of 13 almond (Prunus dulcia) cultivars with known S-genotypes were surveyed by IEF and 2D-PAGE combined with immunoblot and N-terminal amino acid sequence analyses to identify S-RNase associated with gametophytic self-incompatibility (SI) in this plant species. RNase activities corresponding to Sa and Sb two of the four S-alleles tested, were identified by IEF and RNase activity staining. The Sa-RNase band reacted with the anti-S4-serum prepared from Japanese pear (Pyrus serotina); no reaction with the anitserum was observed with the Sb-RNase band. When the Sa-RNase band was excised from an IEF gel stained for RNase activity, subjected to SDS-PAGE, and detected by immunoblotting, it appeared that this band consisted of a single protein that reacted with the anti-S4-serum with Mr of about 28 kDa. With 2D-PAGE and silver staining of the stylar extracts, all four S-proteins could be successfully distinguished from each other in the highly basic zone of the gel
Bibliography:	1997006435 H50 F63 istex:D32FBC5332414859A7E97C4D6D771417CFBFC5BE ark:/67375/HXZ-0F0FML6G-M ArticleID:38.3.304 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0032-0781 1471-9053
DOI:	10.1093/oxfordjournals.pcp.a029167