A comprehensive appraisal of mechanism of anti-CRISPR proteins: an advanced genome editor to amend the CRISPR gene editing

The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein-editing operations. Acr can help with s...

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Bibliographic Details
Published in:	Frontiers in plant science Vol. 14; p. 1164461
Main Authors:	Choudhary, Nisha, Tandi, Dipty, Verma, Rakesh Kumar, Yadav, Virendra Kumar, Dhingra, Naveen, Ghosh, Tathagata, Choudhary, Mahima, Gaur, Rajarshi K, Abdellatif, Magda H, Gacem, Amel, Eltayeb, Lienda Bashier, Alqahtani, Mohammed S, Yadav, Krishna Kumar, Jeon, Byong-Hun
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 23-06-2023
Subjects:	Acr inhibitors anti-CRISPR CRISPR-Cas complex DNA binding inhibition genetic memory genome editing Plant Science anti-CRISPR genome editing DNA cleavage genetic memory DNA binding inhibition CRISPR-Cas complex Acr inhibitors
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Summary:	The development of precise and controlled CRISPR-Cas tools has been made possible by the discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPRs (Acrs). The Acr protein has the ability to control off-targeted mutations and impede Cas protein-editing operations. Acr can help with selective breeding, which could help plants and animals improve their valuable features. In this review, the Acr protein-based inhibitory mechanisms that have been adopted by several Acrs, such as (a) the interruption of CRISPR-Cas complex assembly, (b) interference with target DNA binding, (c) blocking of target DNA/RNA cleavage, and (d) enzymatic modification or degradation of signalling molecules, were discussed. In addition, this review emphasizes the applications of Acr proteins in the plant research.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 Edited by: Shri Mohan Jain, University of Helsinki, Finland These authors have contributed equally to this work Reviewed by: Suprasanna Penna, Amity University, Mumbai, India; Taras P. Pasternak, Miguel Hernández University of Elche, Spain; Hyun Ho Park, Chung-Ang University, Republic of Korea
ISSN:	1664-462X 1664-462X
DOI:	10.3389/fpls.2023.1164461