Search Results - "Tanaka, Charlene K."
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Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry
Published in Proteome science (11-02-2011)“…Wheat flour is one of the world's major food ingredients, in part because of the unique end-use qualities conferred by the abundant glutamine- and proline-rich…”
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Silencing of omega-5 gliadins in transgenic wheat eliminates a major source of environmental variability and improves dough mixing properties of flour
Published in BMC plant biology (24-12-2014)“…The end-use quality of wheat flour varies as a result of the growth conditions of the plant. Among the wheat gluten proteins, the omega-5 gliadins have been…”
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Specific Nongluten Proteins of Wheat Are Novel Target Antigens in Celiac Disease Humoral Response
Published in Journal of proteome research (02-01-2015)“…While the antigenic specificity and pathogenic relevance of immunologic reactivity to gluten in celiac disease have been extensively researched, the immune…”
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Extraction of wheat endosperm proteins for proteome analysis
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (15-04-2007)“…Total protein extracts of wheat endosperm are widely used for the analysis of the highly abundant gliadins and glutenins. In this review, the most popular…”
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Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria
Published in Proceedings of the National Academy of Sciences - PNAS (24-02-2004)“…Mitochondria contain thioredoxin (Trx), a regulatory disulfide protein, and an associated flavoenzyme, NADP Trx reductase, which provide a link to NADPH in the…”
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6
Developmental changes in the metabolic protein profiles of wheat endosperm
Published in Proteomics (Weinheim) (01-04-2005)“…A combined two‐dimensional gel electrophoresis‐mass spectrometry approach was utilized to identify over 250 proteins of wheat (Triticum aestivum L., cv. Butte…”
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Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm
Published in Plant science (Limerick) (01-05-2003)“…The effect of high temperature on starch accumulation, starch granule populations, and expression of genes encoding key enzymes for starch biosynthesis was…”
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Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE
Published in Proteome science (04-08-2011)“…Mineral nutrition during wheat grain development has large effects on wheat flour protein content and composition, which in turn affect flour quality and…”
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Thioredoxin targets of developing wheat seeds identified by complementary proteomic approaches
Published in Phytochemistry (Oxford) (01-06-2004)“…Proteomics in combination with fluorescence (A) and affinity chromatography (B) isolation procedures led to the identification of 68 thioredoxin target…”
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10
Unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics
Published in FEBS letters (17-07-2003)“…Application of a thiol-specific probe, monobromobimane, with proteomics and enzyme assays led to the identification of 23 thioredoxin targets in the starchy…”
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Thioredoxin reduction alters the solubility of proteins of wheat starchy endosperm: An early event in cereal germination
Published in Plant and cell physiology (01-04-2004)“…A KCl-soluble, albumin/globulin fraction of wheat (Triticum aestivum L.) starchy endosperm was further separated into a methanol-insoluble fraction that…”
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Assessment of the Allergenic Potential of Transgenic Wheat (Triticum aestivum) with Reduced Levels of ω5-Gliadins, the Major Sensitizing Allergen in Wheat-Dependent Exercise-Induced Anaphylaxis
Published in Journal of agricultural and food chemistry (28-10-2015)“…The ω5-gliadins are the major sensitizing allergens in wheat-dependent exercise-induced anaphylaxis (WDEIA). In this study, two-dimensional immunoblot analysis…”
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13
Protein composition of wheat gluten polymer fractions determined by quantitative two-dimensional gel electrophoresis and tandem mass spectrometry
Published in Proteome science (11-02-2014)“…Certain wheat gluten proteins form large protein polymers that are extractable in 0.5% SDS only after sonication. Although there is a strong relationship…”
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Quantitative proteomic analysis of wheat grain proteins reveals differential effects of silencing of omega-5 gliadin genes in transgenic lines
Published in Journal of cereal science (01-03-2014)“…Novel wheat lines with altered flour compositions can be used to decipher the roles of specific gluten proteins in flour quality. Grain proteins from…”
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Effect of high temperature on albumin and globulin accumulation in the endosperm proteome of the developing wheat grain
Published in Journal of cereal science (2009)“…The accumulation of KCl-soluble/methanol-insoluble albumins and globulins was investigated in the endosperm of developing wheat ( Triticum aestivum, L. cv…”
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Thioredoxin-Linked Proteins Are Reduced during Germination of Medicago truncatula Seeds
Published in Plant physiology (Bethesda) (01-07-2007)“…Germination of cereals is accompanied by extensive change in the redox state of seed proteins. Proteins present in oxidized form in dry seeds are converted to…”
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Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86
Published in Proteome science (22-02-2013)“…Flour quality is largely determined by the gluten proteins, a complex mixture of proteins consisting of high molecular weight-glutenin subunits (HMW-GS), low…”
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18
Proteome mapping of mature pollen of Arabidopsis thaliana
Published in Proteomics (Weinheim) (01-12-2005)“…The male gametophyte of Arabidopsis is a three‐celled pollen grain that is thought to contain almost all the mRNAs needed for germination and rapid pollen tube…”
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Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis
Published in Plant physiology (Bethesda) (01-07-1986)“…A plasma membrane-enriched fraction prepared from barley roots was analyzed by two-dimensional gel electrophoresis. Four methods of sample solubilization were…”
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Modification of vital wheat gluten with phosphoric acid to produce high free swelling capacity
Published in Journal of applied polymer science (15-01-2014)“…ABSTRACT Wheat gluten reacts with phosphoric acid in the presence of urea to produce natural superabsorbent gels. Fourier Transform Infra‐red (FT‐IR)…”
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