The role of IL-10 in immune responses against Pseudomonas aeruginosa during acute lung infection

Pseudomonas aeruginosa is considered an opportunistic pathogen of great clinical importance. The clearance of this bacterium occurs through recognition of the pathogen by innate immune system receptors, leading to a lung inflammatory response. However, this response must be controlled via immunoregu...

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Published in:	Cell and tissue research Vol. 383; no. 3; pp. 1123 - 1133
Main Authors:	Belo, Valéria Aparecida, Pereira, Jéssica Assis, Souza, Sara Franchin D., Tana, Fernanda de Lima, Pereira, Bruna P., Lopes, Débora de Oliveira, Ceron, Carla S., Novaes, Rômulo D., Corsetti, Patrícia Paiva, de Almeida, Leonardo Augusto
Format:	Journal Article
Language:	English
Published:	Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2021 Springer Springer Nature B.V
Subjects:	Analysis Biomedical and Life Sciences Biomedicine Bronchopulmonary infection Gelatinase A Gelatinase B Health aspects Human Genetics Immune clearance Immune response Immunoregulation Infection Infections Inflammation Innate immunity Interleukin 10 Interleukin 6 Lung diseases Mice Molecular Medicine Opportunist infection Pathogens Proteomics Pseudomonas aeruginosa Regular Article Trachea Tumor necrosis factor-α Virulence (Microbiology) Inflammatory response Interleukin-10 Immunoregulation MMP-9 Lung infection P. aeruginosa
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Summary:	Pseudomonas aeruginosa is considered an opportunistic pathogen of great clinical importance. The clearance of this bacterium occurs through recognition of the pathogen by innate immune system receptors, leading to a lung inflammatory response. However, this response must be controlled via immunoregulatory pathways. In this study, we evaluate the role of endogenous murine IL-10 after acute infection with the virulent strain P. aeruginosa PA14. To assess the role of IL-10, intratracheal infection with the PA14 strain was performed in C57BL/6 or IL-10 KO mice. The PA14 strain was recovered in both types of animals, although IL-10 KO mice presented a higher number of viable bacteria in the lung when compared to the C57BL/6 group. Histopathological and stereological analyses showed that IL-10 KO mice had higher tissue damage and inflammatory infiltrate when compared to control animals. The activity of MMP-9 but not MMP-2, as well as IL-6 and TNF-α expression, were augmented in the lungs of infected animals and was much more evident in IL-10 KO animals when compared to the other analyzed groups. This work indicates that endogenous IL-10 control P. aeruginosa infection, the expression of pro-inflammatory genes, MMP-9 activity and histopathological processes of the infectious process in question.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0302-766X 1432-0878
DOI:	10.1007/s00441-020-03308-4