Search Results - "Tan‐Wilson, Anna"
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Mobilization of seed protein reserves
Published in Physiologia plantarum (01-05-2012)“…The mobilization of seed storage proteins upon seed imbibition and germination is a crucial process in the establishment of the seedling. Storage proteins fold…”
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Peptide nucleic acid probe for protein affinity purification based on biotin–streptavidin interaction and peptide nucleic acid strand hybridization
Published in Analytical biochemistry (01-02-2015)“…We describe a new method for protein affinity purification that capitalizes on the high affinity of streptavidin for biotin but does not require dissociation…”
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3
Electrophoretic transfer protein zymography
Published in Analytical biochemistry (15-04-2011)“…Zymography detects and characterizes proteolytic enzymes by electrophoresis of protease-containing samples into a nonreducing sodium dodecyl…”
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4
Seed storage proteins as a system for teaching protein identification by mass spectrometry in biochemistry laboratory
Published in Biochemistry and molecular biology education (01-03-2013)“…Mass spectrometry (MS) has become an important tool in studying biological systems. One application is the identification of proteins and peptides by the…”
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Effect of embryonic axis removal and exogenous calcium on carboxypeptidase I of mung bean seedling cotyledons
Published in Physiologia plantarum (01-07-2001)“…Removal of the embryonic axis prevents the normal decline of carboxypeptidase (Cpase) I in mung bean seedling cotyledons. Cpase I activity and protein, the…”
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College Students' Views of Work-Life Balance in STEM Research Careers: Addressing Negative Preconceptions
Published in CBE life sciences education (01-09-2015)“…In career discussions, female undergraduates said that if they were to attend graduate school in science, technology, engineering, and mathematics (STEM) and…”
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New serine carboxypeptidase in mung bean seedling cotyledons
Published in Journal of plant physiology (01-10-2003)“…Two serine carboxypeptidases (EC 3.4.16.5) were purified from mung bean seedling cotyledons. Sequences of tryptic peptides derived from the 42.5 kD enzyme…”
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Preparing Graduate Students and Undergraduates for Interdisciplinary Research
Published in Bioscience (01-04-2015)“…Colleges and universities need to provide students with training and experience in (a) interdisciplinary research, (b) the fuzzy areas of responsible conduct…”
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Survey of the proteolytic activities degrading the Kunitz trypsin inhibitor and glycinin in germinating soybeans (Glycine max)
Published in Plant physiology (Bethesda) (01-10-1988)“…The cotyledons of the soybean (Glycine max [L.] Merrill cv Amsoy 71) were examined for proteolytic activities capable of degrading soybean seed proteins. Three…”
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10
Proteases catalyzing vicilin cleavage in developing pea (Pisum sativum L.) seeds
Published in Journal of plant physiology (01-05-2018)“…•Processing of vicilin in immature pea seeds is due to two different proteases.•The proteases show activity against vicilin between pH 4–6.•Cleavage in the…”
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Proteolysis of the peanut allergen Ara h 1 by an endogenous aspartic protease
Published in Plant physiology and biochemistry (01-11-2015)“…The 7S and 11S globulins of peanuts are subjected to proteolysis two days after seed imbibition, with Ara h 1 and the arachin acidic chains being among the…”
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The PA domain is crucial for determining optimum substrate length for soybean protease C1: Structure and kinetics correlate with molecular function
Published in Plant physiology and biochemistry (01-04-2012)“…A subtilisin-like enzyme, soybean protease C1 (EC 3.4.21.25), initiates the degradation of the β-conglycinin storage proteins in early seedling growth…”
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13
Transfer Zymography
Published in Methods in molecular biology (Clifton, N.J.) (2017)“…The technique described here, transfer zymography, was developed to overcome two limitations of conventional zymography. When proteolytic enzymes are resolved…”
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14
Isolation and partial characterization of a subtilisin inhibitor from the mung bean (Vigna radiata)
Published in Plant physiology (Bethesda) (01-09-1989)“…The subtilisin inhibitior (MBSI-A) from the mung bean (Vigna radiata (L.) Wilczek) seed has been purified to homogeneity. MBSI-A consists of a single…”
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15
Role of vacuolar membrane proton pumps in the acidification of protein storage vacuoles following germination
Published in Plant physiology and biochemistry (01-07-2016)“…During soybean (Glycine max (L.) Merrill) seed development, protease C1, the proteolytic enzyme that initiates breakdown of the storage globulins β-conglycinin…”
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Mobilization of seed protein reserves: Plant Proteases
Published in Physiologia plantarum (2012)Get full text
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17
Phytochrome A Mediates Rapid Red Light-Induced Phosphorylation of Arabidopsis FAR-RED ELONGATED HYPOCOTYL1 in a Low Fluence Response
Published in The Plant cell (01-02-2009)“…Phytochrome A (phyA) is the primary photoreceptor for mediating the far-red high irradiance response in Arabidopsis thaliana. FAR-RED ELONGATED HYPOCOTYL1…”
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An Undergraduate STEM Interdisciplinary Research Program: Factors Predictive of Students' Plans for Careers in STEM
Published in Journal of STEM education (01-01-2020)“…In a year-long undergraduate research program, life science majors were paired with majors in the other STEM disciplines of computer science, engineering,…”
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Different rates of metabolism of soybean proteinase inhibitors during germination [Glycine max]
Published in Plant physiology (Bethesda) (01-08-1982)“…During germination, the content of the major Bowman-Birk proteinase inhibitor (BB-E) in the cotyledons of soybean (Glycine max [L.] Merrill cv. Fiskeby V)…”
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Characterization of the proteinase that initiates the degradation of the trypsin inhibitor in germinating mung beans (Vigna radiata)
Published in Plant physiology (Bethesda) (01-05-1987)“…The proteinase (proteinase F) responsible for the initial proteolysis of the mung bean (Vigna radiata) trypsin inhibitor (MBTI) during germination has been…”
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