Search Results - "Tampé, R"

'Traceless' tracing of proteins - high-affinity trans-splicing directed by a minimal interaction pair by Braner, M, Kollmannsperger, A, Wieneke, R, Tampé, R

“…Protein trans-splicing mediated by split inteins is a powerful technique for site-specific protein modification. Despite recent developments there is still an…”
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Chemical modification of proteins by insertion of synthetic peptides using tandem protein trans-splicing by Khoo, K. K., Galleano, I., Gasparri, F., Wieneke, R., Harms, H., Poulsen, M. H., Chua, H. C., Wulf, M., Tampé, R., Pless, S. A.

“…Manipulation of proteins by chemical modification is a powerful way to decipher their function. However, most ribosome-dependent and semi-synthetic methods…”
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Nanomolar affinity protein trans-splicing monitored in real-time by fluorophore-quencher pairs by Braner, M, Wieneke, R, Tampé, R

“…High background originating from non-reacted, 'always-on' fluorescent probes remains a key unsolved problem in life science since washing procedures are not…”
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Vapor Phase Exchange of Self-Assembled Monolayers for Engineering of Biofunctional Surfaces by Kankate, L, Aguf, A, Großmann, H, Schnietz, M, Tampé, R, Turchanin, A, Gölzhäuser, A

“…We show that 4′-nitro-1,1′-biphenyl-4-thiol self-assembled monolayers (NBPT SAMs) on gold can be exchanged with 11-(mercapto­undecyl)­triethylene glycol…”
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Live-cell labeling of endogenous proteins with nanometer precision by transduced nanobodies by Klein, A, Hank, S, Raulf, A, Joest, E F, Tissen, F, Heilemann, M, Wieneke, R, Tampé, R

“…Accurate labeling of endogenous proteins for advanced light microscopy in living cells remains challenging. Nanobodies have been widely used for antigen…”
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Optical control of the antigen translocation by synthetic photo-conditional viral inhibitors by Braner, M, Koller, N, Knauer, J, Herbring, V, Hank, S, Wieneke, R, Tampé, R

“…The immune system makes use of major histocompatibility complex class I (MHC I) molecules to present peptides to other immune cells, which can evoke an immune…”
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Production of Recombinant and Tagged Proteins in the Hyperthermophilic Archaeon Sulfolobus solfataricus by ALBERS, S.-V, JONUSCHEIT, M, DINKELAKER, S, URICH, T, KLETZIN, A, TAMPE, R, DRIESSEN, A. J. M, SCHLEPER, C

“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Molecular Self-Assembly, Chemical Lithography, and Biochemical Tweezers: A Path for the Fabrication of Functional Nanometer-Scale Protein Arrays by Turchanin, A., Tinazli, A., El-Desawy, M., Großmann, H., Schnietz, M., Solak, H. H., Tampé, R., Gölzhäuser, A.

“…Electron‐induced chemical lithography combined with self‐assembled monolayers and multivalent chelators for high‐affinity capturing of His‐tagged proteins are…”
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Molecular mechanisms of fitness compensation in drug resistance-associated NS3 protease variants in hepatitis C by Dultz, G, Shimakami, T, Antes, I, Zeuzem, S, Lange, C, Tampé, R, Welsch, C

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Lipid mono- and bilayer supported on polymer films: composite polymer-lipid films on solid substrates by Kühner, M., Tampé, R., Sackmann, E.

“…We report the deposition of lipid monolayers and bilayers on polyacrylamide films deposited by radical chain reaction onto solid substrates in aqueous…”
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Molecular Organization of Histidine-Tagged Biomolecules at Self-Assembled Lipid Interfaces Using a Novel Class of Chelator Lipids by Dietrich, Christian, Schmitt, Lutz, Tampe, Robert

“…In molecular biology, the expression of fusion proteins is a very useful and well-established technique for the identification and one-step purification of…”
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Force-tuned avidity of spike variant-ACE2 interactions viewed on the single-molecule level by Zhu, Rong, Canena, Daniel, Sikora, Mateusz, Klausberger, Miriam, Seferovic, Hannah, Mehdipour, Ahmad Reza, Hain, Lisa, Laurent, Elisabeth, Monteil, Vanessa, Wirnsberger, Gerald, Wieneke, Ralph, Tampé, Robert, Kienzl, Nikolaus F., Mach, Lukas, Mirazimi, Ali, Oh, Yoo Jin, Penninger, Josef M., Hummer, Gerhard, Hinterdorfer, Peter

“…Recent waves of COVID-19 correlate with the emergence of the Delta and the Omicron variant. We report that the Spike trimer acts as a highly dynamic molecular…”
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The macromolecular peptide-loading complex in MHC class I-dependent antigen presentation by Koch, J, Tampé, R

“…A challenging task for the adaptive immune system of vertebrates is to identify and eliminate intracellular antigens. Therefore a highly specialized antigen…”
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Author Correction: Force-tuned avidity of spike variant-ACE2 interactions viewed on the single-molecule level by Zhu, Rong, Canena, Daniel, Sikora, Mateusz, Klausberger, Miriam, Seferovic, Hannah, Mehdipour, Ahmad Reza, Hain, Lisa, Laurent, Elisabeth, Monteil, Vanessa, Wirnsberger, Gerald, Wieneke, Ralph, Tampé, Robert, Kienzl, Nikolaus F., Mach, Lukas, Mirazimi, Ali, Oh, Yoo Jin, Penninger, Josef M., Hummer, Gerhard, Hinterdorfer, Peter

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Charge determination of membrane molecules in polymer-supported lipid layers by Dietrich, Christian, Tampé, Robert

“…A method for two-dimensional micro-electrophoresis and charge determination of fluorescence-labeled membrane molecules in lipid layers is presented. Therefore,…”
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Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids by Brink, G, Schmitt, L, Tampé, R, Sackmann, E

“…We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine…”
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Allosteric Crosstalk between Peptide-Binding, Transport, and ATP Hydrolysis of the ABC Transporter TAP by Gorbulev, Stanislav, Abele, Rupert, Tampé, Robert

“…The transporter associated with antigen processing (TAP) is essential for intracellular transport of protein fragments into the endoplasmic reticulum for…”
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Function of the transport complex TAP in cellular immune recognition by Abele, Rupert, Tampé, Robert

“…The transporter associated with antigen processing (TAP) is essential for peptide loading onto major histocompatibility complex (MHC) class I molecules by…”
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Immune escape of melanoma: First evidence of structural alterations in two distinct components of the MHC class I antigen processing pathway by SELIGER, Barbara, RITZ, Ulrike, ABELE, Rupert, BOCK, Michaela, TAMPE, Robert, SUTTER, Gert, DREXLER, Ingo, HUBER, Christoph, FERRONE, Soldano

“…Sequence analyses of the transporter associated with antigen processing (TAP) in tumor cell lines with deficient MHC class I surface expression identified a bp…”
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Kinetics of Antigenic Peptide Binding to the Class II Major Histocompatibility Molecule I-Ad by Tampe, Robert, McConnell, Harden M.

“…Using high-performance size-exclusion chromatography and fluorescence spectroscopy, we investigated the kinetics of fluorescent peptide reactions with…”
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