Quantitative studies of Fc receptors on bovine leukemic blood lymphocytes: characterization by binding of homologous IgG1 and IgG2

Receptors for IgG1 and IgG2 (FcR) on peripheral blood lymphocytes from cows with chronic lymphocytic leukemia (CLL) were detected by their ability to bind homologous IgG1 and IgG2 in fluorometric binding assay. Scatchard plots at 4 degrees C demonstrated that IgG1 bound the same number of FcR per ce...

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Bibliographic Details
Published in:	Veterinary immunology and immunopathology Vol. 13; no. 1-2; p. 111
Main Authors:	Dikiniene, N P, Miliukiene, V V, Tamosiũnas, V J
Format:	Journal Article
Language:	English
Published:	Netherlands 01-09-1986
Subjects:	Animals Cattle Cattle Diseases - immunology Fluorescein-5-isothiocyanate Fluoresceins Immunoglobulin G - isolation & purification Immunoglobulin G - metabolism Kinetics Leukemia, Lymphoid - immunology Leukemia, Lymphoid - veterinary Lymphocytes - immunology Receptors, Fc - analysis Receptors, Fc - metabolism Receptors, IgG Thiocyanates
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Summary:	Receptors for IgG1 and IgG2 (FcR) on peripheral blood lymphocytes from cows with chronic lymphocytic leukemia (CLL) were detected by their ability to bind homologous IgG1 and IgG2 in fluorometric binding assay. Scatchard plots at 4 degrees C demonstrated that IgG1 bound the same number of FcR per cell (3.12 +/- 0.69 X 10(5)) as IgG2 (2.89 +/- 0.69 X 10(5)). The receptors bound IgG2 with an affinity of 4.09 +/- 1.08 X 10(5) 1/M and IgG1 with an affinity of 2.73 +/- 0.55 X 10(5) 1/M, although the difference was not of statistical significance (0.1 greater than P greater than 0.05). Inhibition studies demonstrated that the two ligands could inhibit each other. It might be assumed that FcR for the two subclasses were identical.
ISSN:	0165-2427
DOI:	10.1016/0165-2427(86)90053-X