Use of reverse-transcriptase polymerase chain reaction (RT-PCR) for carcinoembryonic antigen, cytokeratin 19, and maspin in the detection of tumor cells in leukapheresis products from patients with breast cancer: comparison with immunocytochemistry

Use of reverse-transcriptase polymerase chain reaction (RT-PCR) for carcinoembryonic antigen, cytokeratin 19, and maspin in the detection of tumor cells in leukapheresis products from patients with breast cancer: comparison with immunocytochemistry

This study evaluates the role of reverse-transcriptase polymerase chain reaction (RT-PCR) assay for carcinoembryonic antigen (CEA), cytokeratin 19 (CK19), and maspin transcripts to identify breast cancer cells (BCC) in leukapheresis products (LP) collected from breast cancer (BC) patients and compar...

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Bibliographic Details
Published in:Journal of hematotherapy Vol. 8; no. 1; p. 53
Main Authors: López-Guerrero, J A, Gilabert, P B, González, E B, Sanz Alonso, M A, Pérez, J P, Talens, A S, Oraval, E A, de la Rubia Comos, J, Boix, S B
Format: Journal Article
Language:English
Published: United States 01-02-1999
Subjects:
Biomarkers, Tumor - analysis
Breast Neoplasms - blood
Breast Neoplasms - chemistry
Breast Neoplasms - pathology
Breast Neoplasms - therapy
Carcinoembryonic Antigen - analysis
Carcinoembryonic Antigen - genetics
False Positive Reactions
Female
Genes, Tumor Suppressor
Humans
Immunohistochemistry
Keratins - analysis
Keratins - genetics
Leukapheresis
Neoplastic Cells, Circulating
Proteins - analysis
Proteins - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Sensitivity and Specificity
Serpins - analysis
Serpins - genetics
Statistics as Topic
Tumor Cells, Cultured
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Summary:This study evaluates the role of reverse-transcriptase polymerase chain reaction (RT-PCR) assay for carcinoembryonic antigen (CEA), cytokeratin 19 (CK19), and maspin transcripts to identify breast cancer cells (BCC) in leukapheresis products (LP) collected from breast cancer (BC) patients and compares these results with those obtained using immunocytochemistry (IC). Eighty-four LP obtained from 33 patients with stage II-III BC and control subjects without BC were screened for the presence of BCC by IC and CK19, CEA, and maspin expression using RT-PCR. CEA RT-PCR and IC were the only specific markers, as no false positives were detected in any patients without BC. CK19 RT-PCR gave 11% false positives, whereas maspin RT-PCR with 25% was the most unspecific marker. In LP from BC patients, positive results were observed in 70% and 63% for CK19 and CEA RT-PCR, respectively. For maspin RT-PCR, this percentage was 22%, and for IC it was 17%. There was a good correlation between the CEA and CK19 RT-PCR (p = 0.018). No correlation between CEA and CK19 RT-PCR and IC was found, and although 5 of the 6 IC+ samples were CEA+/CK19+, great discrepancies in the group of IC- samples were observed. Our data suggest that RT-PCR assays for CEA and, to a lesser extent, for CK19 have more sensitivity and specificity than IC to detect BCC in LP.
ISSN:1061-6128
DOI:10.1089/106161299320578