Genome and antigenic analysis of influenza A (H3N2) viruses isolated from an epidemic in a closed community of Carmelite nuns

Eighteen influenza A (H3N2) viruses were isolated during a single outbreak in a closed community of Carmelite nuns. Serological analysis of the virus haemagglutinin (HAs), using a panel of monoclonal antibodies, demonstrated antigenic microheterogeneity. In contrast, no significant biochemical diffe...

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Bibliographic Details
Published in:	Journal of medical virology Vol. 31; no. 2; p. 112
Main Authors:	Donatelli, I, Takhonova, A M, Klimov, A I, Ghendon, Y Z, Oxford, J S
Format:	Journal Article
Language:	English
Published:	United States 01-06-1990
Subjects:	Adult Antigenic Variation Antigens, Viral - genetics Disease Outbreaks Female Genes, Viral Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral - analysis Hemagglutinins, Viral - genetics Humans Influenza A virus - classification Influenza A virus - genetics Influenza A virus - immunology Influenza A virus - isolation & purification Influenza A Virus, H3N2 Subtype Influenza, Human - epidemiology Influenza, Human - microbiology RNA, Viral - analysis Rome - epidemiology Social Isolation Viral Structural Proteins - genetics Rome
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Summary:	Eighteen influenza A (H3N2) viruses were isolated during a single outbreak in a closed community of Carmelite nuns. Serological analysis of the virus haemagglutinin (HAs), using a panel of monoclonal antibodies, demonstrated antigenic microheterogeneity. In contrast, no significant biochemical differences were detected in viral genes by RNA:RNA hybridisation or in structural or nonstructural polypeptides analysed by high-resolution polyacrylamide gel electrophoresis (PAGE) or by limited proteolysis followed by PAGE. Influenza A (H3N2) viruses isolated in the vicinity of the convent were distinguishable from each other and from the epidemic viruses isolated in the convent both antigenically and biochemically.
ISSN:	0146-6615
DOI:	10.1002/jmv.1890310208