Interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with Prevotella intermedia lipopolysaccharide

Interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with Prevotella intermedia lipopolysaccharide

Interleukin (IL)-8 mRNA expression was investigated in human dental pulp fibroblast cultures after stimulation with lipopolysaccharide (LPS) prepared from Prevotella intermedia and inflammatory cytokines. The expression of IL-8 mRNA and the release of IL-8 induced by P. intermedia LPS in pulpal fibr...

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Bibliographic Details
Published in:Journal of endodontics Vol. 22; no. 1; p. 9
Main Authors: Nagaoka, S, Tokuda, M, Sakuta, T, Taketoshi, Y, Tamura, M, Takada, H, Kawagoe, M
Format: Journal Article
Language:English
Published: United States 01-01-1996
Subjects:
Adolescent
Blotting, Northern
Cells, Cultured
Cytokines - pharmacology
Dental Pulp - cytology
Dental Pulp - immunology
Dental Pulp - metabolism
Dose-Response Relationship, Drug
Fibroblasts - drug effects
Fibroblasts - immunology
Fibroblasts - metabolism
Gene Expression
Humans
Interleukin-8 - biosynthesis
Lipid A - pharmacology
Lipopolysaccharides - immunology
Lipopolysaccharides - pharmacology
Male
Prevotella intermedia - immunology
RNA, Messenger - analysis
Salmonella - immunology
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Summary:Interleukin (IL)-8 mRNA expression was investigated in human dental pulp fibroblast cultures after stimulation with lipopolysaccharide (LPS) prepared from Prevotella intermedia and inflammatory cytokines. The expression of IL-8 mRNA and the release of IL-8 induced by P. intermedia LPS in pulpal fibroblast cultures were detected by Northern blot analysis and ELISA, respectively. The sufficient concentration of P. intermedia LPS on the IL-8 mRNA expression was 0.1 microgram/ml in pulpal fibroblast cultures. IL-8 mRNA levels began to increase after 2 h of exposure, reached a maximum at 4 to 8 h, and declined after 48 h, reaching the unstimulated level by 60 h. IL-8 production by the pulpal fibroblasts began to increase after 8 h of exposure upon stimulation with 10 microgram/ml of P. intermedia LPS. By contrast Salmonella LPS and synthetic lipid A did not increase IL-8 mRNA concentrations in pulpal fibroblast cultures. Recombinant human IL-1 alpha, beta, and tumor necrosis factor-alpha were capable of stimulating these cells to express IL-8 mRNA but natural human interferon-beta, gamma, and recombinant human IL-6 were incapable in our assay. These results suggest that pulpal fibroblasts are immunoresponsive cells and can elaborate IL-8 upon stimulation with P. intermedia LPS.
ISSN:0099-2399
DOI:10.1016/S0099-2399(96)80228-7