Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast

Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast

The complexity of chromatin dynamics is orchestrated by several active processes. In fission yeast, the centromeres are clustered around the spindle pole body (SPB) and oscillate in a microtubule- and adenosine triphosphate (ATP)-dependent manner. However, whether and how SPB oscillation are affecte...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications Vol. 511; no. 4; pp. 820 - 825
Main Authors: Ito, Hiroaki, Sugawara, Takeshi, Shinkai, Soya, Mizukawa, Satoshi, Kondo, Ayaka, Senda, Hisamichi, Sawai, Kengo, Ito, Koki, Suzuki, Sayaka, Takaine, Masakatsu, Yoshida, Satoshi, Imamura, Hiromi, Kitamura, Kenji, Namba, Toshinori, Tate, Shin-ichi, Ueno, Masaru
Format: Journal Article
Language:English
Published: United States Elsevier Inc 16-04-2019
Subjects:
Adenosine triphosphate
Adenosine Triphosphate - metabolism
Ammonium chloride
Ammonium Chloride - metabolism
Centromere - metabolism
Fission yeast
Glucose
Glucose - metabolism
Schizosaccharomyces - cytology
Schizosaccharomyces - metabolism
Spindle Pole Bodies - metabolism
Spindle pole body dynamics
PSD
Fission yeast
SPB
EMM
ms
Glucose
YE
NH4Cl
Ammonium chloride
3D
Spindle pole body dynamics
NaN3
ATP
Adenosine triphosphate
MSD
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The complexity of chromatin dynamics is orchestrated by several active processes. In fission yeast, the centromeres are clustered around the spindle pole body (SPB) and oscillate in a microtubule- and adenosine triphosphate (ATP)-dependent manner. However, whether and how SPB oscillation are affected by different environmental conditions remain poorly understood. In this study, we quantitated movements of the SPB component, which colocalizes with the centromere in fission yeast. We found that SPB movement was significantly reduced at low glucose concentrations. Movement of the SPB was also affected by the presence of ammonium chloride. Power spectral analysis revealed that periodic movement of the SPB is disrupted by low glucose concentrations. Measurement of ATP levels in living cells by quantitative single-cell imaging suggests that ATP levels are not the only determinant of SPB movement. Our results provide novel insight into how SPB movement is regulated by cellular energy status and additional factors such as the medium nutritional composition. •SPB movement is decreased at low glucose concentrations.•SPB movement is increased in the presence of ammonium chloride.•Periodic SPB movement is disrupted at low glucose concentrations.•Fluorescent ATP indicator “QUEEN” can be used in fission yeast.•ATP levels are a major, but not the only determinant of the SPB movement.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2019.02.128