Search Results - "Takahashi, Melissa K."

Creating small transcription activating RNAs by Chappell, James, Takahashi, Melissa K, Lucks, Julius B

“…RNA has been used in a variety of synthetic biology circuits but never as a transcriptional activator. Two design strategies using synthetic and natural…”
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Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components by Pardee, Keith, Green, Alexander A., Takahashi, Melissa K., Braff, Dana, Lambert, Guillaume, Lee, Jeong Wook, Ferrante, Tom, Ma, Duo, Donghia, Nina, Fan, Melina, Daringer, Nichole M., Bosch, Irene, Dudley, Dawn M., O’Connor, David H., Gehrke, Lee, Collins, James J.

“…The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here,…”
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A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers by Takahashi, Melissa K., Tan, Xiao, Dy, Aaron J., Braff, Dana, Akana, Reid T., Furuta, Yoshikazu, Donghia, Nina, Ananthakrishnan, Ashwin, Collins, James J.

“…There is a need for large-scale, longitudinal studies to determine the mechanisms by which the gut microbiome and its interactions with the host affect human…”
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A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future by Chappell, James, Watters, Kyle E, Takahashi, Melissa K, Lucks, Julius B

“…•We review recent progress in RNA synthetic biology.•New advances in synthetic RNA regulator design have created high-performing/functioning parts.•New…”
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Identifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Cell-Free Transcription–Translation Platform by Tsai, Min Jen, Zambrano, Raphael Angelo I., Susas, Jeremiah Lyn, Silva, Lizette, Takahashi, Melissa K.

“…Bacterial small RNAs (sRNAs) regulate many important physiological processes in cells, including antibiotic resistance and virulence genes, through…”
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A modular strategy for engineering orthogonal chimeric RNA transcription regulators by Takahashi, Melissa K, Lucks, Julius B

“…Antisense RNA transcription attenuators are a key component of the synthetic biology toolbox, with their ability to serve as building blocks for both signal…”
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Bacterial metabolic state more accurately predicts antibiotic lethality than growth rate by Lopatkin, Allison J., Stokes, Jonathan M., Zheng, Erica J., Yang, Jason H., Takahashi, Melissa K., You, Lingchong, Collins, James J.

“…Growth rate and metabolic state of bacteria have been separately shown to affect antibiotic efficacy 1 – 3 . However, the two are interrelated as bacterial…”
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Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers by Takahashi, Melissa K, Tan, Xiao, Dy, Aaron J

“…The gut microbiome and its interactions with the host have been shown to affect several aspects of human health and disease. Investigations to elucidate these…”
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Characterizing and prototyping genetic networks with cell-free transcription–translation reactions by Takahashi, Melissa K., Hayes, Clarmyra A., Chappell, James, Sun, Zachary Z., Murray, Richard M., Noireaux, Vincent, Lucks, Julius B.

“…[Display omitted] •Usage of cell-free (TX–TL) systems for characterizing genetic networks are described.•We characterize an RNA transcription cascade in…”
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Towards detection of SARS-CoV-2 RNA in human saliva: A paper-based cell-free toehold switch biosensor with a visual bioluminescent output by Hunt, J. Porter, Zhao, Emily Long, Free, Tyler J., Soltani, Mehran, Warr, Chandler A., Benedict, Alex B., Takahashi, Melissa K., Griffitts, Joel S., Pitt, William G., Bundy, Bradley C.

“…Illustration of the assembly, distribution, and point-of-care use of a rapidly-deployable, cell-free COVID-19 biosensor: 1) Assemble: Assembling CFPS reagents…”
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The centrality of RNA for engineering gene expression by Chappell, James, Takahashi, Melissa K., Meyer, Sarai, Loughrey, David, Watters, Kyle E., Lucks, Julius

“…Synthetic biology holds promise as both a framework for rationally engineering biological systems and a way to revolutionize how we fundamentally understand…”
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BioBits™ Explorer: A modular synthetic biology education kit by Huang, Ally, Nguyen, Peter Q, Stark, Jessica C, Takahashi, Melissa K, Donghia, Nina, Ferrante, Tom, Dy, Aaron J, Hsu, Karen J, Dubner, Rachel S, Pardee, Keith, Jewett, Michael C, Collins, James J

“…Hands-on demonstrations greatly enhance the teaching of science, technology, engineering, and mathematics (STEM) concepts and foster engagement and exploration…”
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Engineering a Functional Small RNA Negative Autoregulation Network with Model-Guided Design by Hu, Chelsea Y, Takahashi, Melissa K, Zhang, Yan, Lucks, Julius B

“…RNA regulators are powerful components of the synthetic biology toolbox. Here, we expand the repertoire of synthetic gene networks built from these regulators…”
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Rapidly Characterizing the Fast Dynamics of RNA Genetic Circuitry with Cell-Free Transcription–Translation (TX-TL) Systems by Takahashi, Melissa K, Chappell, James, Hayes, Clarmyra A, Sun, Zachary Z, Kim, Jongmin, Singhal, Vipul, Spring, Kevin J, Al-Khabouri, Shaima, Fall, Christopher P, Noireaux, Vincent, Murray, Richard M, Lucks, Julius B

“…RNA regulators are emerging as powerful tools to engineer synthetic genetic networks or rewire existing ones. A potential strength of RNA networks is that they…”
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Using in-cell SHAPE-Seq and simulations to probe structure-function design principles of RNA transcriptional regulators by Takahashi, Melissa K, Watters, Kyle E, Gasper, Paul M, Abbott, Timothy R, Carlson, Paul D, Chen, Alan A, Lucks, Julius B

“…Antisense RNA-mediated transcriptional regulators are powerful tools for controlling gene expression and creating synthetic gene networks. RNA transcriptional…”
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