Chinese medicine induces neurite outgrowth in PC12 mutant cells incapable of differentiation

During continuous culture of neural PC12 cells, we obtained a drug-hypersensitive PC12 mutant cell that showed high stimulation of neurite outgrowth by various drugs. When several Chinese medicines such as shu-jing-huo-xie-tang and Wu-Ling-San were provided to these PC12 mutant cells, the frequency...

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Bibliographic Details
Published in:	The American journal of Chinese medicine (1979) Vol. 30; no. 2-3; p. 287
Main Authors:	Kano, Yoshio, Takaguchi, Shinichiro, Nohno, Tsutomu, Hiragami, Fukumi, Kawamura, Kenji, Iwama, Michael K, Miyamoto, Kanji, Takehara, Manabu
Format:	Journal Article
Language:	English
Published:	Singapore 2002
Subjects:	Animals Dose-Response Relationship, Drug Drugs, Chinese Herbal - administration & dosage Drugs, Chinese Herbal - pharmacology Drugs, Chinese Herbal - therapeutic use MAP Kinase Signaling System - drug effects Nerve Growth Factor - pharmacology Neurites - drug effects PC12 Cells - drug effects Phytotherapy Plants, Medicinal Rats Sciatica - prevention & control
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Summary:	During continuous culture of neural PC12 cells, we obtained a drug-hypersensitive PC12 mutant cell that showed high stimulation of neurite outgrowth by various drugs. When several Chinese medicines such as shu-jing-huo-xie-tang and Wu-Ling-San were provided to these PC12 mutant cells, the frequency of nerve growth factor (NGF)-induced neurite outgrowth increased approximately 30-fold compared to NGF alone. Neurite outgrowth induced by NGF in PC12 cells is accompanied by sustained activation of mitogen-activated protein kinase (MAPK); however, these Chinese medicines did not induce MAPK activity. The findings thus indicate that certain Chinese medicines may induce neurite outgrowth by a novel mechanism which is distinct from the NGF-activated pathway in PC12 mutant cells.
ISSN:	0192-415X
DOI:	10.1142/s0192415x02000260