Immunolocalization of myocilin protein in the anterior eye of normal and primary open-angle glaucomatous dogs

The presence of myocilin was investigated in a colony of Beagles, a canine model for inherited primary open-angle glaucoma (POAG). The myocilin protein was localized in the normal and glaucomatous canine eyes by immunohistochemistry and immunocytochemistry. Paraffin- and plastic-embedded specimens f...

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Bibliographic Details
Published in:	Veterinary ophthalmology Vol. 10; no. s1; pp. 28 - 37
Main Authors:	Hart, H, Samuelson, D.A, Tajwar, H, MacKay, E.O, Lewis, P.A, Kallberg, M, Gelatt, K.N
Format:	Journal Article
Language:	English
Published:	Malden, USA Malden, USA : Blackwell Publishing Inc 01-11-2007 Blackwell Publishing Inc
Subjects:	animal models Animals Beagle bioaccumulation biochemical mechanisms Case-Control Studies Cytoskeletal Proteins - metabolism dog dog diseases Dog Diseases - metabolism Dogs epidemiological studies eye Eye Proteins - metabolism eyes genetic disorders glaucoma Glaucoma, Open-Angle - metabolism Glaucoma, Open-Angle - veterinary glycoproteins Glycoproteins - metabolism histopathology immunocytochemistry immunohistochemistry Immunohistochemistry - veterinary inheritance (genetics) mutation myocilin pathogenesis primary open-angle glaucoma tissue distribution Uvea - metabolism
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Summary:	The presence of myocilin was investigated in a colony of Beagles, a canine model for inherited primary open-angle glaucoma (POAG). The myocilin protein was localized in the normal and glaucomatous canine eyes by immunohistochemistry and immunocytochemistry. Paraffin- and plastic-embedded specimens from the anterior uveas of 10 Beagles with inherited glaucoma (3 months to 13 years old) and 6 age-matched normal dogs were sectioned, and were then incubated with primary antibody, rabbit polyclonal antihuman MYOC IgG, overnight at 4 °C. Specimens were incubated with secondary antibody with one of the following: biotinylated link followed by peroxidase-labeled streptavidin and then by substrate-chromogen for light microscopy; fluorescent marker Texas red; or 18 nm colloidal gold-labeled goat antirabbit IgG for transmission electron microscopy. With normal, pre- and early glaucomatous canine specimens, cell membranes of smooth muscle cells of the iris and ciliary body stained positively, as well as most resident stromal and vascular endothelial cells. The cytoplasm of cells within the nonpigmented ciliary epithelium of the ciliary body processes stained intensely, being weaker along the pars plana. Trabecular meshwork (TM) cells and surrounding extracellular matrix labeled, as well as the sclera adjacent to the angular aqueous plexus. In specimens with moderate and advanced glaucoma, greater intensity of staining was observed within TM cells and adjacent sclera, and portions of the nonpigmented epithelium of the ciliary processes. Fibrinous material labeled intensely within the posterior chamber. Myocilin in the normal and glaucomatous canine eye was successfully immunolocalized. These findings with regard to the normal eye are nearly identical to those previously reported in humans, and support the original hypothesis that there is an increase in both accumulation and localization of myocilin in glaucomatous canine eyes. It also supports the possibility that changes in the activity of myocilin within the aqueous humor outflow pathway of individuals with spontaneous glaucoma are associated with the rise of intraocular pressure and subsequent development of this disease, but may not be the primary event in the initial raise in intraocular pressure in POAG in the Beagle.
Bibliography:	http://dx.doi.org/10.1111/j.1463-5224.2007.00517.x ark:/67375/WNG-X5D4CNNX-M ArticleID:VOP517 istex:F64695B6049E1ED9223E5D925F342EC6F4E83E5F ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1463-5216 1463-5224
DOI:	10.1111/j.1463-5224.2007.00517.x