Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees

Roles of cell walls and intracellular contents in supercooling capability of xylem parenchyma cells of boreal trees

The supercooling capability of xylem parenchyma cells (XPCs) in boreal hardwood species differs depending not only on species, but also season. In this study, the roles of cell walls and intracellular contents in supercooling capability of XPCs were examined in three boreal hardwood species, Japanes...

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Published in:Physiologia plantarum Vol. 148; no. 1; pp. 25 - 35
Main Authors: Kasuga, Jun, Endoh, Keita, Yoshiba, Megumi, Taido, Ippei, Arakawa, Keita, Uemura, Matsuo, Fujikawa, Seizo
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-05-2013
Blackwell
Wiley Subscription Services, Inc
Subjects:
Acclimatization
Biological and medical sciences
Cell Wall - physiology
Cold Temperature
Fagus - physiology
Fagus - ultrastructure
Fundamental and applied biological sciences. Psychology
Intracellular Fluid - physiology
Microbiology
Morus - physiology
Morus - ultrastructure
Plant physiology and development
Trees - physiology
Trees - ultrastructure
Xylem - physiology
Xylem - ultrastructure
Xylem
Plant physiology
Content
Parenchyma
Supercooling
Intracellular
Cell
Cell wall
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Summary:The supercooling capability of xylem parenchyma cells (XPCs) in boreal hardwood species differs depending not only on species, but also season. In this study, the roles of cell walls and intracellular contents in supercooling capability of XPCs were examined in three boreal hardwood species, Japanese beech, katsura tree and mulberry, whose supercooling capability differs largely depending on species and season. XPCs in these species harvested in winter and summer were treated by rapid freezing and thawing (RFT samples) or by RFT with further washing (RFTW samples) to remove intracellular contents from XPCs in order to examine the roles of cell walls in supercooling. RFT samples were also treated with glucose solution (RFTG samples) to examine roles of intracellular contents in supercooling. The supercooling capabilities of these samples were examined by differential thermal analysis after ultrastructural observation of XPCs by a cryo‐scanning electron microscope to confirm effects of the above treatments. XPCs in RFTW samples showed a large reduction in supercooling capability to similar temperatures regardless of species or season. On the other hand, XPCs in RFTG samples showed a large increase in supercooling capability to similar temperatures regardless of species or season. These results indicate that although cell walls have an important role in maintenance of supercooling, change in supercooling capability of XPCs is induced by change in intracellular contents, but not by change in cell wall properties.
Bibliography:ArticleID:PPL1678
istex:250765E6CBDFA2444EC1A42468EDB470EBC11C0D
ark:/67375/WNG-7035FDDN-M
These authors contributed equally to this article.
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0031-9317
1399-3054
DOI:10.1111/j.1399-3054.2012.01678.x