Fed-batch coculture of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae for effective production of kefiran

In a batch coculture of kefiran-producing lactic acid bacteria Lactobacillus kefiranofaciens and lactate-assimilating yeast Saccharomyces cerevisiae, lactate accumulation in the medium was observed, which inhibited kefiran production. To enhance kefiran productivity by preventing lactate accumulatio...

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Bibliographic Details
Published in:	Journal of bioscience and bioengineering Vol. 103; no. 6; pp. 557 - 562
Main Authors:	Tada, Shiori, Katakura, Yoshio, Ninomiya, Kazuaki, Shioya, Suteaki
Format:	Journal Article
Language:	English
Published:	Amsterdarm Elsevier B.V 01-06-2007 Elsevier Science Elsevier Limited
Subjects:	Biological and medical sciences Biotechnology CELL CULTURE coculture Coculture Techniques - methods CULTIVO DE CELULAS CULTURE DE CELLULE fed batch culture Feedback Fermentation Fundamental and applied biological sciences. Psychology KEFIR kefiran Kinetics LACTATE LACTATES LACTATOS Lactic Acid - metabolism LACTOBACILLUS Lactobacillus - metabolism Lactobacillus kefiranofaciens Models, Biological Polysaccharides - biosynthesis SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - metabolism Lactobacillus kefiranofaciens kefiran fed batch culture coculture Saccharomyces cerevisiae Lactic acid bacteria Yeast Fungi Production Bacteria Lactobacillaceae Ascomycetes Culture Semicontinuous Thallophyta Lactobacillus
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Summary:	In a batch coculture of kefiran-producing lactic acid bacteria Lactobacillus kefiranofaciens and lactate-assimilating yeast Saccharomyces cerevisiae, lactate accumulation in the medium was observed, which inhibited kefiran production. To enhance kefiran productivity by preventing lactate accumulation, we conducted lactose-feeding batch operation with feedforward/feedback control during the coculture, so that the lactate production rate of L. kefiranofaciens was balanced with the lactate consumption rate of S. cerevisiae. The lactate concentration was maintained at less than 6 g l −1 throughout the fed-batch coculture using a 5 l jar fermentor, although the concentration reached 33 g l −1 in the batch coculture. Kefiran production was increased to 6.3 g in 102 h in the fed-batch coculture, whereas 4.5 g kefiran was produced in 97 h in the batch coculture. The kefiran yield on lactose basis was increased up to 0.033 g g −1 in the fed-batch coculture, whereas that in the batch coculture was 0.027 g g −1.
Bibliography:	2007008703 Q02 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1389-1723 1347-4421
DOI:	10.1263/jbb.103.557