Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry

Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry

Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w D...

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Bibliographic Details
Published in:Journal of applied phycology Vol. 33; no. 1; pp. 263 - 274
Main Authors: Taborda, Tiago, Moniz, Patricia, Delgado dos Reis, Alberto José, Saraiva Lopes da Silva, Maria Teresa
Format: Journal Article
Language:English
Published: Dordrecht Springer 01-02-2021
Springer Netherlands
Springer Nature B.V
Subjects:
Acetates
Acetic acid
Alternative carbon source
Biodiesel fuels
Biofuels
Biomedical and Life Sciences
Carbon
Carbon sources
Crypthecodinium cohnii
Ecology
Effluents
Fatty acids
Flow cytometry
Freshwater & Marine Ecology
Glucose
Glycerol
Life Sciences
Lipids
Low cost
Microalgae
Molasses
Plant Physiology
Plant Sciences
Substrates
Sugarcane
Syrups & sweeteners
Vinegar
Flow cytometry
Lipids
Pure substrates
Low-cost substrates
DHA
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Summary:Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g(-1) DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g(-1), respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-020-02304-z