Search Results - "TURNER, Stephen W"

Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data by Chin, Chen-Shan, Alexander, David H, Marks, Patrick, Klammer, Aaron A, Drake, James, Heiner, Cheryl, Clum, Alicia, Copeland, Alex, Huddleston, John, Eichler, Evan E, Turner, Stephen W, Korlach, Jonas

“…Unlike hybrid approaches that use multiple libraries for de novo assembly, the hierarchical genome-assembly process uses data from only a single long-read SMRT…”
Get full text

Direct detection of DNA methylation during single-molecule, real-time sequencing by Travers, Kevin J, Olivares, Eric C, Flusberg, Benjamin A, Korlach, Jonas, Lee, Jessica H, Webster, Dale R, Turner, Stephen W, Clark, Tyson A

“…Polymerase kinetics observed during single-molecule, real-time sequencing depend on the methylation status of the DNA template. Measurement of kinetic…”
Get full text

A Promising Integration of SDN and Blockchain for IoT Networks: A Survey by Turner, Stephen W., Karakus, Murat, Guler, Evrim, Uludag, Suleyman

“…The state of computer network technologies has continually advanced at a rapid pace. Software Defined Networking (SDN) and Blockchain (BC) have emerged as…”
Get full text

Short-stay urgent hospital admissions of children with convulsions: A mixed methods exploratory study to inform out of hospital care pathways by Malcolm, Cari, Hoddinott, Pat, King, Emma, Dick, Smita, Kyle, Richard, Wilson, Philip, France, Emma, Aucott, Lorna, Turner, Stephen W

“…To inform interventions focused on safely reducing urgent paediatric short stay admissions (SSAs) for convulsions. Routinely acquired administrative data from…”
Get full text

Reconstructing complex regions of genomes using long-read sequencing technology by Huddleston, John, Ranade, Swati, Malig, Maika, Antonacci, Francesca, Chaisson, Mark, Hon, Lawrence, Sudmant, Peter H, Graves, Tina A, Alkan, Can, Dennis, Megan Y, Wilson, Richard K, Turner, Stephen W, Korlach, Jonas, Eichler, Evan E

“…Obtaining high-quality sequence continuity of complex regions of recent segmental duplication remains one of the major challenges of finishing genome…”
Get full text

Real-time tRNA transit on single translating ribosomes at codon resolution by Puglisi, Joseph D, Uemura, Sotaro, Aitken, Colin Echeverría, Korlach, Jonas, Flusberg, Benjamin A, Turner, Stephen W

“…Translation by the ribosome occurs by a complex mechanism involving the coordinated interaction of multiple nucleic acid and protein ligands. Here we use…”
Get full text

Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing by Anvar, Seyed Yahya, Allard, Guy, Tseng, Elizabeth, Sheynkman, Gloria M, de Klerk, Eleonora, Vermaat, Martijn, Yin, Raymund H, Johansson, Hans E, Ariyurek, Yavuz, den Dunnen, Johan T, Turner, Stephen W, 't Hoen, Peter A C

“…The multifaceted control of gene expression requires tight coordination of regulatory mechanisms at transcriptional and post-transcriptional level. Here, we…”
Get full text

Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine by Song, Chun-Xiao, Clark, Tyson A, Lu, Xing-Yu, Kislyuk, Andrey, Dai, Qing, Turner, Stephen W, He, Chuan, Korlach, Jonas

“…The DNA modification 5-hydroxymethylcytosine has recently been implicated in several biological processes. Enrichment by selective chemical labeling in…”
Get full text

Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences by Cao, Bo, Chen, Chao, DeMott, Michael S., Cheng, Qiuxiang, Clark, Tyson A., Xiong, Xiaolin, Zheng, Xiaoqing, Butty, Vincent, Levine, Stuart S., Yuan, George, Boitano, Matthew, Luong, Khai, Song, Yi, Zhou, Xiufen, Deng, Zixin, Turner, Stephen W., Korlach, Jonas, You, Delin, Wang, Lianrong, Chen, Shi, Dedon, Peter C.

“…Bacterial phosphorothioate (PT) DNA modifications are incorporated by Dnd proteins A-E and often function with DndF-H as a restriction-modification (R-M)…”
Get full text

Short stay hospital admissions for an acutely unwell child: A qualitative study of outcomes that matter to parents and professionals by Malcolm, Cari, King, Emma, France, Emma, Kyle, Richard G, Kumar, Simita, Dick, Smita, Wilson, Philip, Aucott, Lorna, Turner, Stephen W, Hoddinott, Pat

“…Numbers of urgent short stay admissions (SSAs) of children to UK hospitals are rising rapidly. This paper reports on experiences of SSAs from the perspective…”
Get full text

Comprehensive methylome characterization of Mycoplasma genitalium and Mycoplasma pneumoniae at single-base resolution by Lluch-Senar, Maria, Luong, Khai, Lloréns-Rico, Verónica, Delgado, Javier, Fang, Gang, Spittle, Kristi, Clark, Tyson A, Schadt, Eric, Turner, Stephen W, Korlach, Jonas, Serrano, Luis

“…In the bacterial world, methylation is most commonly associated with restriction-modification systems that provide a defense mechanism against invading foreign…”
Get full text

Maternal vitamin d intake during pregnancy and early childhood wheezing by DEVEREUX, Graham, LITONJUA, Augusto A, TURNER, Stephen W, CRAIG, Leone C. A, MCNEILL, Geraldine, MARTINDALE, Sheelagh, HELMS, Peter J, SEATON, Anthony, WEISS, Scott T

“…Maternal intake of vitamin D in pregnancy is a potentially modifiable but understudied risk factor for the development of asthma in children. We investigated…”
Get full text

High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence by Chen, Jin, Dalal, Ravindra V., Petrov, Alexey N., Tsai, Albert, O'Leary, Seán E., Chapin, Karen, Cheng, Janice, Ewan, Mark, Hsiung, Pei-Lin, Lundquist, Paul, Turner, Stephen W., Hsu, David R., Puglisi, Joseph D.

“…Zero-mode waveguides provide a powerful technology for studying single-molecule real-time dynamics of biological systems at physiological ligand…”
Get full text

Global methylation state at base-pair resolution of the Caulobacter genome throughout the cell cycle by Kozdon, Jennifer B, Melfi, Michael D, Luong, Khai, Clark, Tyson A, Boitano, Matthew, Wang, Susana, Zhou, Bo, Gonzalez, Diego, Collier, Justine, Turner, Stephen W, Korlach, Jonas, Shapiro, Lucy, McAdams, Harley H

“…The Caulobacter DNA methyltransferase CcrM is one of five master cell-cycle regulators. CcrM is transiently present near the end of DNA replication when it…”
Get full text

Enhanced 5-methylcytosine detection in single-molecule, real-time sequencing via Tet1 oxidation by Clark, Tyson A, Lu, Xingyu, Luong, Khai, Dai, Qing, Boitano, Matthew, Turner, Stephen W, He, Chuan, Korlach, Jonas

“…DNA methylation serves as an important epigenetic mark in both eukaryotic and prokaryotic organisms. In eukaryotes, the most common epigenetic mark is…”
Get full text

Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription by Vilfan, Igor D, Tsai, Yu-Chih, Clark, Tyson A, Wegener, Jeffrey, Dai, Qing, Yi, Chengqi, Pan, Tao, Turner, Stephen W, Korlach, Jonas

“…Zero-mode waveguides (ZMWs) are photonic nanostructures that create highly confined optical observation volumes, thereby allowing single-molecule-resolved…”
Get full text

Selective Aluminum Passivation for Targeted Immobilization of Single DNA Polymerase Molecules in Zero-Mode Waveguide Nanostructures by Korlach, Jonas, Marks, Patrick J., Cicero, Ronald L., Gray, Jeremy J., Murphy, Devon L., Roitman, Daniel B., Pham, Thang T., Otto, Geoff A., Foquet, Mathieu, Turner, Stephen W.

“…Optical nanostructures have enabled the creation of subdiffraction detection volumes for single-molecule fluorescence microscopy. Their applicability is…”
Get full text

Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures by Richards, Christopher I, Luong, Khai, Srinivasan, Rahul, Turner, Stephen W, Dougherty, Dennis A, Korlach, Jonas, Lester, Henry A

“…We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors…”
Get full text

AgIn: measuring the landscape of CpG methylation of individual repetitive elements by Suzuki, Yuta, Korlach, Jonas, Turner, Stephen W, Tsukahara, Tatsuya, Taniguchi, Junko, Qu, Wei, Ichikawa, Kazuki, Yoshimura, Jun, Yurino, Hideaki, Takahashi, Yuji, Mitsui, Jun, Ishiura, Hiroyuki, Tsuji, Shoji, Takeda, Hiroyuki, Morishita, Shinichi

“…Determining the methylation state of regions with high copy numbers is challenging for second-generation sequencing, because the read length is insufficient to…”
Get full text

Associations between smoke-free vehicle legislation and childhood admissions to hospital for asthma in Scotland: an interrupted time-series analysis of whole-population data by Mackay, Daniel F, Turner, Stephen W, Semple, Sean E, Dick, Smita, Pell, Jill P

“…In Scotland, childhood admissions to hospital for asthma fell from March, 2006, after legislation was introduced to prohibit smoking in public places. In…”
Get full text