Comparison of Ca2+-dependent K+ channels in the membrane of smooth muscle cells isolated from adult and foetal human aorta

Comparison of Ca2+-dependent K+ channels in the membrane of smooth muscle cells isolated from adult and foetal human aorta

Ca2+-activated K+ ionic currents in the membrane of cultured smooth muscle cells isolated from foetal and adult human aorta were studied using whole cell and single-channel patch-clamp techniques. Whole cell currents in adult smooth muscle cells were 3-8 times larger than in foetal cells of similar...

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Bibliographic Details
Published in:Pflügers Archiv Vol. 413; no. 1; pp. 8 - 13
Main Authors: BREGESTOVSKI, P. D, PRINTSEVA, O. YU, SEREBRYAKOV, V, STINNAKRE, J, TURMIN, A, ZAMOYSKI, V
Format: Journal Article
Language:English
Published: Heidelberg Springer 01-11-1988
Subjects:
Adult
Aorta - metabolism
Aorta - physiology
Biological and medical sciences
Calcium - metabolism
Cells, Cultured
Fetus - metabolism
Fundamental and applied biological sciences. Psychology
Heart
Humans
Membrane Potentials
Middle Aged
Muscle, Smooth, Vascular - metabolism
Muscle, Smooth, Vascular - physiology
Potassium Channels - metabolism
Vertebrates: cardiovascular system
Human
Transmembrane current
Cell culture
Calcium
Patch clamp method
Blood vessel
Development
Adult
Aorta
Fetus
Cations
Circulatory system
Potassium
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Summary:Ca2+-activated K+ ionic currents in the membrane of cultured smooth muscle cells isolated from foetal and adult human aorta were studied using whole cell and single-channel patch-clamp techniques. Whole cell currents in adult smooth muscle cells were 3-8 times larger than in foetal cells of similar sizes. The elementary conductance and ionic selectivity of single Ca2+-activated K+ were identical for both types of cells. Channel openings occurred in burst, the duration of which was 3-5-fold longer in adult than in foetal cells. The voltage dependency of the channel activating mechanism and the dependency of the mean open time on the Ca2+ concentration on the inner side of the membrane were similar for both types of cells. These results suggest that the main reason for the increase in potassium conductance during development is an alteration in the open time of the Ca2+-activated K+ channels.
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ISSN:0031-6768
1432-2013
DOI:10.1007/BF00581222