Search Results - "TURKOVA, J"
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Hydrazide-functionalized poly(2-hydroxyethyl methacrylate) microspheres for immobilization of horseradish peroxidase
Published in Biotechnology progress (1999)“…Nonporous cross‐linked poly(2‐hydroxyethyl methacrylate‐co‐ethylene dimethacrylate) (poly(HEMA‐co‐EDMA) ) microspheres were prepared by dispersion…”
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Oriented immobilization of chymotrypsin by use of suitable antibodies coupled to a nonporous solid support
Published in Journal of Chromatography A (06-08-1999)“…In order to eliminate the kinetic limitation of chymotryptic hydrolysis of proteins due to diffusion, nonporous hydroxyalkyl methacrylate solid support was…”
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Journal Article Conference Proceeding -
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Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function
Published in Journal of Chromatography B: Biomedical Sciences and Applications (05-02-1999)“…The advantages of oriented immobilization of biologically active proteins are good steric accessibilities of active binding sites and increased stability. This…”
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Book Review Journal Article -
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Influence of salts on the covalent immobilization of proteins to modified copolymers of 2-hydroxyethyl methacrylate with ethylene dimethacrylate
Published in Biotechnology and applied biochemistry (01-02-1988)“…In the study of the covalent immobilization of aminoacylase, thermitase, pepsin, trypsin, chymotrypsin, elastase, subtilisin, penicillinamidohydrolase,…”
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Detection of pathological changes of proteins by peptide mapping after protein digestion by use of oriented immobilized proteinases
Published in Journal of molecular recognition (01-09-1996)“…Diagnostic methods for detecting gastric diseases using chymotryptic digestion of pepsin are discussed. Peptide maps can be prepared using reversed‐phase…”
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Stabilization of trypsin by glycosylation
Published in Biotechnology techniques (01-06-1994)Get full text
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Polyclonal anti-chymotrypsin antibodies suitable for oriented immobilization of chymotrypsin
Published in Biotechnology letters (01-02-1988)Get full text
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Reversed-phase high-performance liquid chromatography of peptides of porcine pepsin prepared by the use of various forms of immobilized α-chymotrypsin
Published in Journal of chromatography. B, Biomedical sciences and applications (25-03-2001)“…Reversed-phase high-performance liquid chromatography (RP-HPLC) separation was used for the comparison of peptide maps of pepsin after its digestions by…”
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Journal Article -
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Immobilization and nonspecific adsorption of proteins to pyrogeneous highly dispersed silicon dioxide
Published in Biotechnology letters (01-08-1987)“…A support based on pyrogeneous silicon dioxide of particle size 0.01 to 0.1/um, modified by 3-(amino)propyl-triethoxysilane and activated by glutaraldehyde was…”
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Specificity of alkaline proteinase of Aspergillus flavus
Published in Biochimica et biophysica acta (01-01-1970)Get more information
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Activation of galactose-containing glycoprotein and solid supports by galactose oxidase in presence of catalase for immobilization purposes
Published in Biotechnology techniques (1990)“…Specific oxidation of D-galactose present in the carbohydrate moiety of glucose oxidase from Aspergillus niger by galactose oxidase in the presence of catalase…”
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Journal Article -
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Purification of anti-chymotrypsin antibodies for the preparation of a bioaffinity matrix with oriented chymotrypsin as immobilized ligand
Published in Journal of chromatography. B, Biomedical sciences and applications (07-02-1997)“…Polyclonal antibodies suitable for the oriented immobilization of chymotrypsin we prepared by chromatography on a bioaffinity matrix which had the enzyme…”
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Oriented immobilization of galactose oxidase to bead and magnetic bead cellulose and poly(HEMA-co-EDMA) and magnetic poly(HEMA-co-EDMA) microspheres
Published in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (25-04-2002)“…In order to obtain an active and stable oxidation reactor for daily use in biochemical laboratory we decided to immobilize galactose oxidase orientedly through…”
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Coupling of ligands to insoluble matrices via aldehyde groups
Published in Biotechnology letters (01-04-1986)Get full text
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SH-proteinase from bean Phaseolus vulgaris var. Perlicka
Published in Biochimica et biophysica acta (22-10-1975)“…An SH-proteinase (EC 3.4.22.-) has been isolated from beans of the species Phaseolus vulgaris var. Perlicka. The enzyme is homogeneous when subjected to disc…”
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Journal Article -
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Carbohydrates as a tool for oriented immobilization of antigens and antibodies
Published in Journal of chromatography (02-02-1990)“…A biospecific sorbent for the isolation of ovalbumin antibodies was prepared by coupling of ovalbumin via its periodate-oxidized carbohydrate moiety to bead…”
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Galactosylation as a tool for the stabilization and immobilization of proteins
Published in Journal of chromatography (24-04-1992)“…This paper presents a brief overview of the role that the carbohydrate moieties of biologically active glycoproteins play in the stabilization and oriented…”
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Affinity chromatography
Published in Journal of chromatography (24-04-1974)Get more information
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Affinity chromatography of proteases of hydroxyalkyl methacrylate gels with covalently attached inhibitors
Published in Journal of chromatography (21-01-1978)“…The efficient isolation of trypsin and chymotrypsin from a crude pancreatic extract was achieved by affinity chromatography on specific adsorbents prepared by…”
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