Search Results - "TUCKER, Chandra"

The HSF-like Transcription Factor TBF1 Is a Major Molecular Switch for Plant Growth-to-Defense Transition by Pajerowska-Mukhtar, Karolina M., Wang, Wei, Tada, Yasuomi, Oka, Nodoka, Tucker, Chandra L., Fonseca, Jose Pedro, Dong, Xinnian

“…Induction of plant immune responses involves significant transcription reprogramming that prioritizes defense over growth-related cellular functions. Despite…”
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Engineering genetically-encoded tools for optogenetic control of protein activity by Liu, Qi, Tucker, Chandra L

“…•Optogenetic tools allow control of protein activity with spatiotemporal precision.•New photosensory domains provide diverse building blocks for optogenetic…”
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Optimized second-generation CRY2–CIB dimerizers and photoactivatable Cre recombinase by Taslimi, Amir, Zoltowski, Brian, Miranda, Jose G, Pathak, Gopal P, Hughes, Robert M, Tucker, Chandra L

“…Identification of minimal functional CRY2–CIB1 domains and mutations that increased CRY2 photocycle lifetimes combined with the development of an improved…”
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An optimized optogenetic clustering tool for probing protein interaction and function by Taslimi, Amir, Vrana, Justin D., Chen, Daniel, Borinskaya, Sofya, Mayer, Bruce J., Kennedy, Matthew J., Tucker, Chandra L.

“…The Arabidopsis photoreceptor cryptochrome 2 (CRY2) was previously used as an optogenetic module, allowing spatiotemporal control of cellular processes with…”
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Rapid blue-light-mediated induction of protein interactions in living cells by Tucker, Chandra L, Kennedy, Matthew J, Hughes, Robert M, Peteya, Leslie A, Schwartz, Joel W, Ehlers, Michael D

“…Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically…”
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Photodimerization systems for regulating protein–protein interactions with light by Spiltoir, Jessica I, Tucker, Chandra L

“…•Photodimerizer systems are similar to chemical dimerizers, allowing control of protein interactions with light.•Photodimerizers can be used to induce and/or…”
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A platform to induce and mature biomolecular condensates using chemicals and light by Hernandez-Candia, Carmen N., Brady, Brian R., Harrison, Evan, Tucker, Chandra L.

“…Biomolecular condensates are membraneless compartments that impart spatial and temporal organization to cells. Condensates can undergo maturation,…”
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Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2 by Pathak, Gopal P, Spiltoir, Jessica I, Höglund, Camilla, Polstein, Lauren R, Heine-Koskinen, Sari, Gersbach, Charles A, Rossi, Jari, Tucker, Chandra L

“…Optogenetic tools allow regulation of cellular processes with light, which can be delivered with spatiotemporal resolution. In previous work, we used…”
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A modular tool to query and inducibly disrupt biomolecular condensates by Hernández-Candia, Carmen N., Pearce, Sarah, Tucker, Chandra L.

“…Dynamic membraneless compartments formed by protein condensates have multifunctional roles in cellular biology. Tools that inducibly trigger condensate…”
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Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent by Meador, Kyle, Wysoczynski, Christina L, Norris, Aaron J, Aoto, Jason, Bruchas, Michael R, Tucker, Chandra L

“…A common mechanism for inducibly controlling protein function relies on reconstitution of split protein fragments using chemical or light-induced dimerization…”
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Optogenetic control of cell function using engineered photoreceptors by Pathak, Gopal P., Vrana, Justin D., Tucker, Chandra L.

“…Over the past decades, there has been growing recognition that light can provide a powerful stimulus for biological interrogation. Light‐actuated tools allow…”
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Photo-SNAP-tag, a Light-Regulated Chemical Labeling System by Cleveland, Joseph D, Tucker, Chandra L

“…Methods that allow labeling and tracking of proteins have been instrumental for understanding their function. Traditional methods for labeling proteins include…”
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Light-mediated control of DNA transcription in yeast by Hughes, Robert M., Bolger, Steven, Tapadia, Hersh, Tucker, Chandra L.

“…A variety of methods exist for inducible control of DNA transcription in yeast. These include the use of native yeast promoters or regulatory elements that are…”
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A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission by Liu, Qi, Sinnen, Brooke L., Boxer, Emma E., Schneider, Martin W., Grybko, Michael J., Buchta, William C., Gibson, Emily S., Wysoczynski, Christina L., Ford, Christopher P., Gottschalk, Alexander, Aoto, Jason, Tucker, Chandra L., Kennedy, Matthew J.

“…Regulated secretion is critical for diverse biological processes ranging from immune and endocrine signaling to synaptic transmission. Botulinum and tetanus…”
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Photo-activatable Cre recombinase regulates gene expression in vivo by Schindler, Suzanne E., McCall, Jordan G., Yan, Ping, Hyrc, Krzystof L., Li, Mingjie, Tucker, Chandra L., Lee, Jin-Moo, Bruchas, Michael R., Diamond, Marc I.

“…Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a…”
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Quantitative proteomics approach for identifying protein-drug interactions in complex mixtures using protein stability measurements by West, Graham M, Tucker, Chandra L, Xu, Tao, Park, Sung Kyu, Han, Xuemei, Yates, John R. III, Fitzgerald, Michael C

“…Knowledge about the protein targets of therapeutic agents is critical for understanding drug mode of action. Described here is a mass spectrometry-based…”
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Optogenetic Control of Gene Expression Using Cryptochrome 2 and a Light-Activated Degron by Hernández-Candia, Carmen N, Tucker, Chandra L

“…Optogenetic tools allow for use of light as an external input to control cellular processes. When applied to regulate the function of transcription factors,…”
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Dual Systems for Enhancing Control of Protein Activity through Induced Dimerization Approaches by Pearce, Sarah, Tucker, Chandra L.

“…To reveal the underpinnings of complex biological systems, a variety of approaches have been developed that allow switchable control of protein function. One…”
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Advances in optogenetic regulation of gene expression in mammalian cells using cryptochrome 2 (CRY2) by Hernández-Candia, Carmen N., Wysoczynski, Christina L., Tucker, Chandra L.

“…•Optogenetic tools allow inducible control of gene expression in mammalian cells.•We describe use of CRY2 to regulate Cre recombinase and transcription…”
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LTP expression mediated by autonomous activity of GluN2B-bound CaMKII by Rumian, Nicole L., Barker, C. Madison, Larsen, Matthew E., Tullis, Jonathan E., Freund, Ronald K., Taslimi, Amir, Coultrap, Steven J., Tucker, Chandra L., Dell’Acqua, Mark L., Bayer, K. Ulrich

“…Learning and memory are thought to require the induction and maintenance of long-term potentiation (LTP) of synaptic strength. LTP induction requires the…”
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