Search Results - "TSUGITA, Akira"

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    Proteome Analysis of Human Vitreous Proteins by Yamane, Ken, Minamoto, Atsushi, Yamashita, Hidetoshi, Takamura, Hiroshi, Miyamoto-Myoken, Yuka, Yoshizato, Katsutoshi, Nabetani, Takuji, Tsugita, Akira, Mishima, Hiromu K

    Published in Molecular & cellular proteomics (01-11-2003)
    “…Purpose: Various protein contents such as enzymes, growth factors, and structural components are responsible for biological activities in organs. We have…”
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    Proteome analysis of mouse brain: Two-dimensional electrophoresis profiles of tissue proteins during the course of aging by Tsugita, Akira, Kawakami, Takao, Uchida, Tatsuya, Sakai, Tomoko, Kamo, Masaharu, Matsui, Takuro, Watanabe, Yu, Morimasa, Tadaomi, Hosokawa, Keiichi, Toda, Tosifusa

    Published in Electrophoresis (01-05-2000)
    “…Mouse brain proteins were isolated from five regions (cerebellum, cerebral cortex, hippocampus, striatum, and cervical spinal cord) at five ages from the 10th…”
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    A Programmable Fragmentation Analysis of Proteins by In-Source Decay in MALDI-TOF Mass Spectrometry by Gao, Jialing, Tsugita, Akira, Takayama, Mitsuo, Xu, Lin

    Published in Analytical chemistry (Washington) (15-03-2002)
    “…Here we describe an algorithm for identifying peptides/proteins of known sequence and unknown peptides from partial spectra generated by an in-source decay…”
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    Two-dimensional electrophoresis of plant proteins and standardization of gel patterns by Tsugita, A, Kamo, M, Kawakami, T, Ohki, Y

    Published in Electrophoresis (1996)
    “…Proteins of two plants, Arabidopsis thaliana and rice (Oryza sativa) were subjected to two-dimensional electrophoresis analysis with two modifications: (i)…”
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    Separation and characterization of rice proteins by Tsugita, A, Kawakami, T, Uchiyama, Y, Kamo, M, Miyatake, N, Nozu, Y

    Published in Electrophoresis (1994)
    “…Rice proteins from nine tissues and one organelle (leaf, chloroplast, stem, root, germ, dark germinated seedling, seed, bran, chaff and callus) were isolated…”
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    A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals by Horikawa, Hiroshi P.M., Saisu, Hideo, Ishizuka, Toru, Sekine, Yoko, Tsugita, Akira, Odani, Shoji, Abe, Teruo

    Published in FEBS letters (13-09-1993)
    “…Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine…”
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    Proteomic analysis of rice leaf, stem and root tissues during growth course by Nozu, Yuzo, Tsugita, Akira, Kamijo, Kenichi

    Published in Proteomics (Weinheim) (01-06-2006)
    “…Rice proteins were isolated from leaf, stem and root tissues, harvesting at 1, 2, 4, 8 and 10 weeks after budding. Each tissue of each age was separately…”
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    A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein by Tsugita, A, Uchida, T, Mewes, H W, Ataka, T

    Published in Journal of biochemistry (Tokyo) (01-12-1987)
    “…A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture…”
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    Additional possible tools for identification of proteins on one- or two-dimensional electrophoresis by Tsugita, A, Kamo, M, Miyazaki, K, Takayama, M, Kawakami, T, Shen, R, Nozawa, T

    Published in Electrophoresis (01-05-1998)
    “…Additional, essentially chemical, identification methods of proteins in polyacrylamide gel electrophoresis are described. Two cleavages of peptide bonds were…”
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    Proteome Analysis of the Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on Murine Testicular Leydig and Sertoli Cells by Uchida, Tatsuya, Ohashi, Yoshiki, Morikawa, Emiko, Tsugita, Akira, Takeda, Ken

    Published in Journal of Health Science (2001)
    “…We treated testicular cell lines Leydig TM3 and Sertoli TM4 with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and used two-dimensional electrophoresis to…”
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    Further study on gas phase acid hydrolysis of protein: improvement of recoveries for tryptophan, tyrosine, and methionine by Yano, H, Aso, K, Tsugita, A

    Published in Journal of biochemistry (Tokyo) (01-10-1990)
    “…A novel method for vapor phase acid hydrolysis of protein suitable for quantitative analysis of tryptophan is presented. The hydrolysis is carried out in vapor…”
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    Analysis of acidic peptides with a matrix-assisted laser desorption/ionization mass spectrometry using positive and negative ion modes with additive monoammonium phosphate by Nabetani, Takuji, Miyazaki, Kenji, Tabuse, Yo, Tsugita, Akira

    Published in Proteomics (Weinheim) (01-08-2006)
    “…Acidic PTMs such as phosphorylation and sulfonation of proteins are known to play important roles in many cellular processes including signal transductions and…”
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    Specific chemical cleavage of asparaginyl and glycyl-glycine bonds in peptides and proteins by anhydrous hydrazine vapor by Miyatake, N, Satake, K, Kamo, M, Tsugita, A

    Published in Journal of biochemistry (Tokyo) (01-02-1994)
    “…Hydrazinolysis of peptide or protein has been used for C-terminal amino acid determination by Akabori et al. (1952). In this study, proteins were reacted with…”
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    Proteomic analysis of protein expression profiles during Caenorhabditis elegans development using two-dimensional difference gel electrophoresis by Tabuse, Yo, Nabetani, Takuji, Tsugita, Akira

    Published in Proteomics (Weinheim) (01-07-2005)
    “…Coordinated protein expression is critical for the normal execution of animal development. To obtain overall proteome profiles during animal development, a…”
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