Search Results - "TSUGITA, Akira"
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The minimum information about a proteomics experiment (MIAPE)
Published in Nature biotechnology (01-08-2007)“…Both the generation and the analysis of proteomics data are now widespread, and high-throughput approaches are commonplace. Protocols continue to increase in…”
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Proteome Analysis of Human Vitreous Proteins
Published in Molecular & cellular proteomics (01-11-2003)“…Purpose: Various protein contents such as enzymes, growth factors, and structural components are responsible for biological activities in organs. We have…”
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Two-dimensional electrophoretic profiling of normal human kidney glomerulus proteome and construction of an extensible markup language (XML)-based database
Published in Proteomics (Weinheim) (01-03-2005)“…To contribute to physiology and pathophysiology of the glomerulus of human kidney, we have launched a proteomic study of human glomerulus, and compiled a…”
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Proteome analysis of mouse brain: Two-dimensional electrophoresis profiles of tissue proteins during the course of aging
Published in Electrophoresis (01-05-2000)“…Mouse brain proteins were isolated from five regions (cerebellum, cerebral cortex, hippocampus, striatum, and cervical spinal cord) at five ages from the 10th…”
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A Programmable Fragmentation Analysis of Proteins by In-Source Decay in MALDI-TOF Mass Spectrometry
Published in Analytical chemistry (Washington) (15-03-2002)“…Here we describe an algorithm for identifying peptides/proteins of known sequence and unknown peptides from partial spectra generated by an in-source decay…”
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Two-dimensional electrophoresis of plant proteins and standardization of gel patterns
Published in Electrophoresis (1996)“…Proteins of two plants, Arabidopsis thaliana and rice (Oryza sativa) were subjected to two-dimensional electrophoresis analysis with two modifications: (i)…”
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Separation and characterization of rice proteins
Published in Electrophoresis (1994)“…Rice proteins from nine tissues and one organelle (leaf, chloroplast, stem, root, germ, dark germinated seedling, seed, bran, chaff and callus) were isolated…”
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A complex of rab3A, SNAP-25, VAMP/synaptobrevin-2 and syntaxins in brain presynaptic terminals
Published in FEBS letters (13-09-1993)“…Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine…”
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Proteomic analysis of rice leaf, stem and root tissues during growth course
Published in Proteomics (Weinheim) (01-06-2006)“…Rice proteins were isolated from leaf, stem and root tissues, harvesting at 1, 2, 4, 8 and 10 weeks after budding. Each tissue of each age was separately…”
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Overview of the HUPO Plasma Proteome Project: Results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database
Published in Proteomics (Weinheim) (01-08-2005)“…HUPO initiated the Plasma Proteome Project (PPP) in 2002. Its pilot phase has (1) evaluated advantages and limitations of many depletion, fractionation, and MS…”
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A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein
Published in Journal of biochemistry (Tokyo) (01-12-1987)“…A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture…”
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Additional possible tools for identification of proteins on one- or two-dimensional electrophoresis
Published in Electrophoresis (01-05-1998)“…Additional, essentially chemical, identification methods of proteins in polyacrylamide gel electrophoresis are described. Two cleavages of peptide bonds were…”
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Proteome Analysis of the Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on Murine Testicular Leydig and Sertoli Cells
Published in Journal of Health Science (2001)“…We treated testicular cell lines Leydig TM3 and Sertoli TM4 with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and used two-dimensional electrophoresis to…”
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Bond-Specific Chemical Cleavages of Peptides and Proteins with Perfluoric Acid Vapors: Novel Peptide Bond Cleavages of Glycyl-Threonine, the Amino Side of Serine Residues and the Carboxyl Side of Aspartic Acid Residues
Published in Journal of biochemistry (Tokyo) (01-01-1997)“…Peptide bond cleavages by vapors composed of various from aqueous solutions of perfluoric acid were studied using synthetic peptides and proteins, and specific…”
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Further study on gas phase acid hydrolysis of protein: improvement of recoveries for tryptophan, tyrosine, and methionine
Published in Journal of biochemistry (Tokyo) (01-10-1990)“…A novel method for vapor phase acid hydrolysis of protein suitable for quantitative analysis of tryptophan is presented. The hydrolysis is carried out in vapor…”
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Guidelines for reporting the use of mass spectrometry in proteomics
Published in Nature biotechnology (01-08-2008)Get full text
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Analysis of acidic peptides with a matrix-assisted laser desorption/ionization mass spectrometry using positive and negative ion modes with additive monoammonium phosphate
Published in Proteomics (Weinheim) (01-08-2006)“…Acidic PTMs such as phosphorylation and sulfonation of proteins are known to play important roles in many cellular processes including signal transductions and…”
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Specific chemical cleavage of asparaginyl and glycyl-glycine bonds in peptides and proteins by anhydrous hydrazine vapor
Published in Journal of biochemistry (Tokyo) (01-02-1994)“…Hydrazinolysis of peptide or protein has been used for C-terminal amino acid determination by Akabori et al. (1952). In this study, proteins were reacted with…”
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Proteomic analysis of protein expression profiles during Caenorhabditis elegans development using two-dimensional difference gel electrophoresis
Published in Proteomics (Weinheim) (01-07-2005)“…Coordinated protein expression is critical for the normal execution of animal development. To obtain overall proteome profiles during animal development, a…”
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