Endophytic colonization ability of two deep-water rice endophytes, Pantoea sp. and Ochrobactrum sp. using green fluorescent protein reporter

Colonization ability of the two endophytic bacteria, isolated from surface sterilized seeds of Jaisurya variety of deep-water rice viz., Pantoea sp. and Ochrobactrum sp., was compared after genetically tagging them with a constitutively expressing green fluorescent protein gene (gfp). Confocal laser...

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Bibliographic Details
Published in:	Biotechnology letters Vol. 26; no. 5; pp. 425 - 429
Main Authors:	VERMA, Subhash C, SINGH, Anamika, CHOWDHURY, Soumitra Paul, TRI, Anil K
Format:	Journal Article
Language:	English
Published:	Dordrecht Springer 01-03-2004 Springer Nature B.V
Subjects:	Biological and medical sciences Biotechnology Cell Division - physiology Colonization Colony Count, Microbial - methods Deep water Endophytes Fluorescence Fundamental and applied biological sciences. Psychology Genes, Reporter Green Fluorescent Proteins Hydroponics Luminescent Proteins - genetics Luminescent Proteins - metabolism Microscopy, Fluorescence - methods Ochrobactrum - cytology Ochrobactrum - genetics Ochrobactrum - growth & development Ochrobactrum - metabolism Oryza - cytology Oryza - microbiology Oryza sativa Pantoea - cytology Pantoea - genetics Pantoea - growth & development Pantoea - metabolism Proteins Recombinant Proteins - metabolism Seedlings Species Specificity Water Microbiology Water Green fluorescent protein Bacteria Endophyte Pantoea Colonization Enterobacteriaceae Rice
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Summary:	Colonization ability of the two endophytic bacteria, isolated from surface sterilized seeds of Jaisurya variety of deep-water rice viz., Pantoea sp. and Ochrobactrum sp., was compared after genetically tagging them with a constitutively expressing green fluorescent protein gene (gfp). Confocal laser scanning microscopy (CLSM) of hydroponically grown seedlings of Jaisurya rice, inoculated with gfp-tagged endophytes, revealed that both Pantoea sp. and Ochrobactrum sp. colonized the intercellular spaces in the root cortex when inoculated separately. Colonization by gfp-tagged Ochrobactrum sp. was severely inhibited when co-inoculated with an equal number (10(5) c.f.u. ml(-1)) of wild type Pantoea sp., but the converse was not true. Pantoea sp. was a more aggressive endophytic colonizer of its host than Ochrobactrum sp. The potential of using GFP reporter and CLSM as tools in evaluating competitive ability of colonization among endophytes is herewith demonstrated.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0141-5492 1573-6776
DOI:	10.1023/b:bile.0000018263.94440.ab