RAPD markers linked to resistance to downy mildew disease in soybean

RAPD markers linked to resistance to downy mildew disease in soybean

To determine and utilize RAPD markers linked to resistance to downymildew incited by Peronospora manshurica in soybean, a resistantcultivar `AGS129' was crossed to a susceptible cultivar `Nakhon Sawan 1'(NS1). F^sub 2^ and BC^sub 1^ populations were advanced from the F^sub 1^ and evaluated...

Full description

Saved in:
Bibliographic Details
Published in:Euphytica Vol. 128; no. 1; pp. 55 - 60
Main Authors: CHOWDHURY, A. K, SRINIVES, P, SAKSOONG, P, TONGPAMNAK, P
Format: Journal Article
Language:English
Published: Dordrecht Springer 01-11-2002
Springer Nature B.V
Subjects:
Agronomy. Soil science and plant productions
Airborne microorganisms
Biological and medical sciences
Cultivars
Deoxyribonucleic acid
Disease resistance
DNA
Fundamental and applied biological sciences. Psychology
Genetics
Genetics and breeding of economic plants
Genomics
Pest resistance
Plant pathogens
Soybeans
Varietal selection. Specialized plant breeding, plant breeding aims
Genetic marker
Mycosis
Plant pathogen
bulked segregant analysis
Phycomycetes
Marker assisted selection
Molecular marker
Line (plant)
Fungi
Dicotyledones
Angiospermae
Near isogenic line
Downy mildew
Thallophyta
near-isogenic lines
Glycine max
Random amplified polymorphic DNA marker
Fungus resistance
Infection
Grain legume
Segregation analysis
Sensitivity resistance
Leguminosae
Peronospora manshurica
Genetic improvement
RAPD markers
Spermatophyta
Cultivar
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine and utilize RAPD markers linked to resistance to downymildew incited by Peronospora manshurica in soybean, a resistantcultivar `AGS129' was crossed to a susceptible cultivar `Nakhon Sawan 1'(NS1). F^sub 2^ and BC^sub 1^ populations were advanced from the F^sub 1^ and evaluatedfor resistance to the disease. χ^sup 2^-test demonstrated that the resistancewas controlled by a single dominant gene (Rpmx). Near-isogenic lines(NILs) and bulked segregant analysis (BSA) were used to identify RAPDmarkers linked to the gene. Six DNA bulks namely F^sub 5^(R), F^sub 5^(S),BC^sub 6^F^sub 3^(R), BC^sub 6^F^sub 3^(S), F^sub 2^(R) and F^sub 2^(S) were set up by pooling equalamount of DNA from 8 randomly selected plants of each disease responsetype. A total of 180 random sequence decamer oligonucleotide primerswere used for RAPD analysis. Primer OPH-02 (5' TCGGACGTGA 3' andOPP-10 (5' TCCCGCCTAC 3') generated OPH-02^sub 1250^ and OPP-10^sub 831^fragments in donor parent and resistant bulks, but not in the recurrentparent and susceptible ones. Co-segregation analysis using 102 segregatingF^sub 2^ progenies confirmed that both markers were linked to the Rpmxgene controlling downy mildew disease resistance with a genetic distance of4.9 cm and 23.1 cm, respectively. Marker OPH-02^sub 1250^ was presentin 13 of 16 resistant soybean cultivars and absent in susceptible cultivars,thus confirming a potential for MAS outside the mapping population.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0014-2336
1573-5060
DOI:10.1023/A:1020635501050