Search Results - "TOKUNAGA, Makio"

Highly inclined thin illumination enables clear single-molecule imaging in cells by Tokunaga, Makio, Imamoto, Naoko, Sakata-Sogawa, Kumiko

“…We describe a simple illumination method of fluorescence microscopy for molecular imaging. Illumination by a highly inclined and thin beam increases image…”
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Regulation of RNA polymerase II activation by histone acetylation in single living cells by Stasevich, Timothy J., Hayashi-Takanaka, Yoko, Sato, Yuko, Maehara, Kazumitsu, Ohkawa, Yasuyuki, Sakata-Sogawa, Kumiko, Tokunaga, Makio, Nagase, Takahiro, Nozaki, Naohito, McNally, James G., Kimura, Hiroshi

“…The interplay of histone acetylation and RNA polymerase II activity is investigated using fluorescence microscopy; acetylation of H3 at Lys 27 enhances the…”
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Live imaging of transcription sites using an elongating RNA polymerase II-specific probe by Uchino, Satoshi, Ito, Yuma, Sato, Yuko, Handa, Tetsuya, Ohkawa, Yasuyuki, Tokunaga, Makio, Kimura, Hiroshi

“…In eukaryotic nuclei, most genes are transcribed by RNA polymerase II (RNAP2), whose regulation is a key to understanding the genome and cell function. RNAP2…”
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Zinc is a novel intracellular second messenger by Yamasaki, Satoru, Sakata-Sogawa, Kumiko, Hasegawa, Aiko, Suzuki, Tomoyuki, Kabu, Koki, Sato, Emi, Kurosaki, Tomohiro, Yamashita, Susumu, Tokunaga, Makio, Nishida, Keigo, Hirano, Toshio

“…Zinc is an essential trace element required for enzymatic activity and for maintaining the conformation of many transcription factors; thus, zinc homeostasis…”
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Multi-color single-molecule tracking and subtrajectory analysis for quantification of spatiotemporal dynamics and kinetics upon T cell activation by Ito, Yuma, Sakata-Sogawa, Kumiko, Tokunaga, Makio

“…The dynamic properties of molecules in living cells are attracting increasing interest. We propose a new method, moving subtrajectory analysis using…”
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RNG105 deficiency impairs the dendritic localization of mRNAs for Na+/K+ ATPase subunit isoforms and leads to the degeneration of neuronal networks by Shiina, Nobuyuki, Yamaguchi, Kazuhiko, Tokunaga, Makio

“…mRNA transport and local translation in dendrites play key roles in use-dependent synaptic modification and in higher-order brain functions. RNG105, an…”
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Slitflow: A Python framework for single-molecule dynamics and localization analysis by Ito, Yuma, Hirose, Masanori, Tokunaga, Makio

“…Single-molecule imaging is a promising method for direct quantification of the dynamics and distribution of biomolecules in living cells. Although numerous…”
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RNA Granule Protein 140 (RNG140), a Paralog of RNG105 Localized to Distinct RNA Granules in Neuronal Dendrites in the Adult Vertebrate Brain by Shiina, Nobuyuki, Tokunaga, Makio

“…RNA granules mediate the transport and local translation of their mRNA cargoes, which regulate cellular processes such as stress response and neuronal synaptic…”
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Nuclear actin activates human transcription factor genes including the OCT4 gene by Yamazaki, Shota, Yamamoto, Koji, Tokunaga, Makio, Sakata-Sogawa, Kumiko, Harata, Masahiko

“…RNA microarray analyses revealed that nuclear actin activated many human transcription factor genes including OCT4, which is required for gene reprogramming…”
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A Novel RNA-Binding Protein in Neuronal RNA Granules: Regulatory Machinery for Local Translation by Shiina, Nobuyuki, Shinkura, Kazumi, Tokunaga, Makio

“…Local translation in neuronal dendrites is an important basis for long-term synaptic plasticity, and RNA granules in the dendrites are involved in the local…”
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A Facile Preparation of Glass-supported Lipid Bilayers for Analyzing Molecular Dynamics by ITO, Yuma, SAKATA-SOGAWA, Kumiko, TOKUNAGA, Makio

“…Many research programs focus on the molecular dynamics of living cells. This research requires cells to be adhered to a substrate while retaining the innate…”
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Ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assembly by Matsumori, Haruka, Watanabe, Kenji, Tachiwana, Hiroaki, Fujita, Tomoko, Ito, Yuma, Tokunaga, Makio, Sakata-Sogawa, Kumiko, Osakada, Hiroko, Haraguchi, Tokuko, Awazu, Akinori, Ochiai, Hiroshi, Sakata, Yuka, Ochiai, Koji, Toki, Tsutomu, Ito, Etsuro, Goldberg, Ilya G, Tokunaga, Kazuaki, Nakao, Mitsuyoshi, Saitoh, Noriko

“…The nucleolus is the site of ribosome assembly and formed through liquid-liquid phase separation. Multiple ribosomal DNA (rDNA) arrays are bundled in the…”
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Conformational changes in inhibitory PAS domain protein associated with binding of HIF-1α and Bcl-xL in living cells by Kasai, Shuya, Kajimoto, Shinji, Ito, Yuma, Saito, Tomo, Yasumoto, Ken-Ichi, Tokunaga, Makio, Sakata-Sogawa, Kumiko, Fukumura, Hiroshi, Sogawa, Kazuhiro

“…Inhibitory PAS domain protein (IPAS) is a dual function protein acting as a transcriptional repressor and as a pro-apoptotic protein. Simultaneous dual-color…”
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Vav links the T cell antigen receptor to the actin cytoskeleton and T cell activation independently of intrinsic Guanine nucleotide exchange activity by Miletic, Ana V, Graham, Daniel B, Sakata-Sogawa, Kumiko, Hiroshima, Michio, Hamann, Michael J, Cemerski, Saso, Kloeppel, Tracie, Billadeau, Daniel D, Kanagawa, Osami, Tokunaga, Makio, Swat, Wojciech

“…T cell receptor (TCR) engagement leads to formation of signaling microclusters and induction of rapid and dynamic changes in the actin cytoskeleton, although…”
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Single-molecule localization-integrated trajectory analysis for the dynamic interaction of chromatin structure and transcription machinery by Ito, Yuma, Tokunaga, Makio

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Single Molecule Imaging of Fluorophores and Enzymatic Reactions Achieved by Objective-Type Total Internal Reflection Fluorescence Microscopy by Tokunaga, Makio, Kitamura, Kazuo, Saito, Kiwamu, Iwane, Atsuko Hikikoshi, Yanagida, Toshio

“…Imaging of single fluorescent molecules has been achieved in a relatively simple manner using objective-type total internal reflection fluorescence microscopy…”
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Simultaneous single-molecule imaging of RNA polymerase II dynamics and chromatin nanostructures in living cells by Ito, Yuma, Tokunaga, Makio

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Vav1 Acidic Region Tyrosine 174 Is Required for the Formation of T Cell Receptor-induced Microclusters and Is Essential in T Cell Development and Activation by Miletic, Ana V., Sakata-Sogawa, Kumiko, Hiroshima, Michio, Hamann, Michael J., Gomez, Timothy S., Ota, Naruhisa, Kloeppel, Tracie, Kanagawa, Osami, Tokunaga, Makio, Billadeau, Daniel D., Swat, Wojciech

“…Vav proteins are multidomain signaling molecules critical for mediating signals downstream of several surface receptors, including the antigen receptors of T…”
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Direct Observation of Multiple and Stochastic Transition States by a Feedback-controlled Single-molecule Force Measurement by FUKAGAWA, Akihiro, HIROSHIMA, MichioA, SAKANE, Isao, TOKUNAGA, Makio

“…To overcome the ensemble-averaging barrier, single-molecule experiments have been performed, but energy landscapes comprising multiple intermediates have not…”
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CLIP-170 is essential for MTOC repositioning during T cell activation by regulating dynein localisation on the cell surface by Lim, Wei Ming, Ito, Yuma, Sakata-Sogawa, Kumiko, Tokunaga, Makio

“…The microtubule-organizing centre (MTOC) is repositioned to the centre of the contacted cell surface, the immunological synapse, during T cell activation…”
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