Extraction of Water-soluble Soybean Polysaccharides under Acidic Conditions

Extraction of Water-soluble Soybean Polysaccharides under Acidic Conditions

We investigated the yield, gelation and sugar composition of water-soluble polysaccharides that had been extracted from soybean cotyledons under acidic conditions. SSPS were easily extracted at several pHs. The highest yield was about twice that reported by Morita et al., after extraction in boiling...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry Vol. 62; no. 12; pp. 2300 - 2305
Main Authors: FURUTA, Hitoshi, TAKAHASHI, Taro, TOBE, Jyunko, KIWATA, Ryosuke, MAEDA, Hirokazu
Format: Journal Article
Language:English
Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998
Japan Society for Bioscience Biotechnology and Agrochemistry
Oxford University Press
Subjects:
acidity
Biological and medical sciences
extraction
Food industries
Fundamental and applied biological sciences. Psychology
polysaccharide
soybean
Use and upgrading of agricultural and food by-products. Biotechnology
Molecular weight distribution
Gelation
Soybean
Hot water
Acidic solution
Grain legume
Water soluble compound
Upgrading
Chemical composition
Extraction
Polysaccharide
By product
Physicochemical properties
soybean
acidity
polysaccharide
extraction
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Summary:We investigated the yield, gelation and sugar composition of water-soluble polysaccharides that had been extracted from soybean cotyledons under acidic conditions. SSPS were easily extracted at several pHs. The highest yield was about twice that reported by Morita et al., after extraction in boiling water, or by Kawamura et al., after extraction in hot water (90°C) with the stepwise addition of ammonium oxalate and 0.5% NaOH. Our results show that the average molecular weight was relatively high for both SSPS extracted in the weakly acidic pH range of 4-6 at 100-120°C and in the strongly acidic pH range of 2-3 at 40-80°C. The water-soluble polysaccharide solutions which were extracted under the former conditions remained fluid, but those which were extracted under the latter conditions gelled. However, the molecular weight distribution and sugar composition were similar for SSPS isolated by using both sets of conditions. Furthermore, water-soluble soybean polysaccharides were found to consist of rhamnogalacturonans, which could endure hydrolysis, and arabinogalactans, which were easily hydrolyzed under acidic conditions.
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ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.62.2300