Search Results - "THOMPSON, Nancy E"

Architecture of RNA Polymerase II and Implications for the Transcription Mechanism by Cramer, Patrick, Bushnell, David A., Fu, Jianhua, Gnatt, Averell L., Maier-Davis, Barbara, Thompson, Nancy E., Burgess, Richard R., Edwards, Aled M., David, Peter R., Kornberg, Roger D.

“…A backbone model of a 10-subunit yeast RNA polymerase II has been derived from x-ray diffraction data extending to 3 angstroms resolution. All 10 subunits…”
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Minimal Promoter Systems Reveal the Importance of Conserved Residues in the B-finger of Human Transcription Factor IIB by Thompson, Nancy E., Glaser, Bryan T., Foley, Katherine M., Burton, Zachary F., Burgess, Richard R.

“…The “B-finger” of transcription factor IIB (TFIIB) is highly conserved and believed to play a role in the initiation process. We performed alanine…”
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Weak protein–protein interactions revealed by immiscible filtration assisted by surface tension by Berry, Scott M., Chin, Emily N., Jackson, Shawn S., Strotman, Lindsay N., Goel, Mohit, Thompson, Nancy E., Alexander, Caroline M., Miyamoto, Shigeki, Burgess, Richard R., Beebe, David J.

“…Biological mechanisms are often mediated by transient interactions between multiple proteins. The isolation of intact protein complexes is essential to…”
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Advances in gentle immunoaffinity chromatography by Burgess, Richard R., Thompson, Nancy E.

“…Immunoaffinity chromatography is one of the most powerful fractionation steps available for protein purification; however, it is often difficult to elute bound…”
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The epitope for the polyol-responsive monoclonal antibody 8RB13 is in the flap-domain of the beta-subunit of bacterial RNA polymerase and can be used as an epitope tag for immunoaffinity chromatography by Stalder, Elizabeth S., Nagy, Lauren H., Batalla, Pilar, Arthur, Terrance M., Thompson, Nancy E., Burgess, Richard R.

“…Polyol-responsive monoclonal antibodies (PR-mAbs) are useful for the purification of proteins in an easy, one step immunoaffinity step. These antibodies allow…”
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Production and characterization of monoclonal antibodies to estrogen-related receptor alpha (ERRα) and use in immunoaffinity chromatography by Esch, Amanda M., Thompson, Nancy E., Lamberski, Jennifer A., Mertz, Janet E., Burgess, Richard R.

“…► We generated a panel of high-affinity monoclonal antibodies (mAbs) to ERRα. ► Nine mAbs detected ERRα in ELISA and Western blot; mAbs isotyped and…”
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Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase by Thompson, Nancy E, Hager, Dayle A, Burgess, Richard R

“…A modified enzyme-linked immunosorbent assay (ELISA) was used to screen monoclonal antibodies (MAbs) that react with Escherichia coli RNA polymerase for the…”
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Identification of Sp2 as a transcriptional repressor of carcinoembryonic antigen-related cell adhesion molecule 1 in tumorigenesis by PHAN, Dillon, CHENG, Chien-Jui, GALFIONE, Matthew, VAKAR-LOPEZ, Funda, TUNSTEAD, James, THOMPSON, Nancy E, BURGESS, Richard R, NAJJAR, Sonia M, YU-LEE, Li-Yuan, LIN, Sue-Hwa

“…Down-regulation of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) tumor suppressor gene expression is common in several malignancies…”
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CDK9 Autophosphorylation Regulates High-Affinity Binding of the Human Immunodeficiency Virus Type 1 Tat–P-TEFb Complex to TAR RNA by Garber, Mitchell E., Mayall, Timothy P., Suess, Eric M., Meisenhelder, Jill, Thompson, Nancy E., Jones, Katherine A.

“…Article Usage Stats Services MCB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Key Concepts of Environmental Sustainability: Knowledge and Confidence Levels of FCS Teachers by Harden, Amy J, Friesen, Carol A, Thompson, Nancy E

“…Family and consumer sciences (FCS) is a logical discipline to promote environmental sustainability within the family because it is recognized as helping people…”
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Immunoaffinity purification and characterization of RNA polymerase from Shewanella oneidensis by Probasco, Mitchell D., Thompson, Nancy E., Burgess, Richard R.

“…Shewanella oneidensis is of particular interest for research because of its unique ability to use a variety of metals as final respiratory electron acceptors…”
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Development of an epitope tag for the gentle purification of proteins by immunoaffinity chromatography: application to epitope-tagged green fluorescent protein by Thompson, Nancy E, Arthur, Terrance M, Burgess, Richard R

“…Polyol-responsive monoclonal antibodies (mAbs) are useful tools for the gentle purification of proteins and protein complexes. These are high-affinity mAbs…”
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Expression and purification of a single-chain variable fragment antibody derived from a polyol-responsive monoclonal antibody by Lamberski, Jennifer A., Thompson, Nancy E., Burgess, Richard R.

“…A previously described polyol-responsive monoclonal antibody (PR-mAb) was converted to a single-chain variable fragment (scFv). This antibody, PR-mAb NT73,…”
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LRET-based HTS of a small-compound library for inhibitors of bacterial RNA polymerase by Glaser, Bryan T, Bergendahl, Veit, Thompson, Nancy E, Olson, Brian, Burgess, Richard R

“…Resistance mechanisms against whole classes of antibiotics are currently developing faster than research generates new structurally different biologically…”
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Expression, purification of, and monoclonal antibodies to sigma factors from Escherichia coli by Anthony, Larry C, Foley, Katherine M, Thompson, Nancy E, Burgess, Richard R

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Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography by Thompson, Nancy E, Foley, Katherine M, Stalder, Elizabeth S, Burgess, Richard R

“…Immunoaffinity chromatography is a powerful tool for purification of proteins and protein complexes. The availability of monoclonal antibodies (mAbs) has…”
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An epitope tag derived from human transcription factor IIB that reacts with a polyol-responsive monoclonal antibody by Duellman, Sarah J, Thompson, Nancy E, Burgess, Richard R

“…Polyol-responsive monoclonal antibodies (PR-mAbs) provide a strategy to purify active, nondenatured proteins by a single-step immunoaffinity chromatography…”
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Antigen-binding properties of monoclonal antibodies reactive with human TATA-binding protein and use in immunoaffinity chromatography by Thompson, Nancy E, Foley, Katherine M, Burgess, Richard R

“…The TATA-binding protein (TBP) plays a central role in the assembly of most eukaryotic transcription initiation complexes. We have characterized 3 monoclonal…”
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A cross-reactive polyol-responsive monoclonal antibody useful for isolation of core RNA polymerase from many bacterial species by Bergendahl, Veit, Thompson, Nancy E, Foley, Kit M, Olson, Brian M, Burgess, Richard R

“…The use of antibodies for protein purification is a powerful technique but the release of the target protein in its active form is often difficult. So called…”
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Purification of protein complexes by immunoaffinity chromatography: application to transcription machinery by Thompson, Nancy E, Jensen, Debra Bridges, Lamberski, Jennifer A, Burgess, Richard R

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