Usefulness of the organ culture system in the in vitro diagnosis of coeliac disease: A multicentre study

Objective. Diagnosis of coeliac disease is based on the presence of villous atrophy which recovers following a gluten-free diet. The presence of circulating antiendomysial antibodies as well as their disappearance after a gluten-free diet supports the diagnosis. It has also been demonstrated that an...

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Published in:	Scandinavian journal of gastroenterology Vol. 41; no. 2; pp. 186 - 190
Main Authors:	Picarelli, Antonio, Di Tola, Marco, Sabbatella, Luigi, Cristina Anania, Maria, Calabrò, Antonio, Renzi, Daniela, Bai, Julio Cesar, Sugai, Emilia, Carroccio, Antonio, Prima, Lidia Di, Bardella, Maria Teresa, Barisani, Donatella, Ribes-Koninckx, Carmen, Aliaga, Ester Donat, Gasparin, Maurizio, Bravi, Enzo, THE MULTICENTRE ORGAN CULTURE SYSTEM STUDY GROUP
Format:	Journal Article
Language:	English
Published:	Copenhagen Informa UK Ltd 01-02-2006 Oslo Taylor & Francis Stockholm Scandinavian University Press
Subjects:	Adolescent Adult Aged Antiendomysial antibodies Argentina - epidemiology Autoantibodies - immunology Biological and medical sciences Biopsy Celiac Disease - epidemiology Celiac Disease - immunology Celiac Disease - pathology Child Child, Preschool coeliac disease diagnosis Endoscopy, Gastrointestinal Female Gastroenterology. Liver. Pancreas. Abdomen histology Humans Incidence Infant Italy - epidemiology Male Medical sciences Middle Aged organ culture Organ Culture Techniques - methods Other diseases. Semiology Reproducibility of Results Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Italy Argentina Antibody Histology Coeliac disease In vitro Intestinal malabsorption Gastroenterology organ culture Antiendomysial antibodies Digestive diseases Intestinal disease Diagnosis Culture Organ
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Summary:	Objective. Diagnosis of coeliac disease is based on the presence of villous atrophy which recovers following a gluten-free diet. The presence of circulating antiendomysial antibodies as well as their disappearance after a gluten-free diet supports the diagnosis. It has also been demonstrated that antiendomysial antibodies are detectable in supernatants of cultured intestinal biopsies from patients with coeliac disease. The objective of this study was to compare the histology and antiendomysial antibodies in culture supernatants of intestinal biopsies to validate the in vitro organ culture system as a future diagnostic tool for coeliac disease. Material and methods. Seventy-five antiendomysial serum-positive patients on a gluten-containing diet were evaluated. Patients underwent endoscopy with 5 biopsy fragments: 3 for histology, 1 cultured with and the other without gliadin-peptide activator. Antiendomysial antibodies were evaluated in all culture supernatants. Results. Sixty-eight patients had evidence of villous atrophy, while 73 out of 75 were positive to the organ culture system. The agreement rate between organ culture and histology results was 94%. Conclusions. As all the centres participating in the study obtained good agreement between organ culture and histology results, the new system could be considered a reliable tool for the diagnosis of coeliac disease. Nevertheless, it is possible to highlight cases with an organ culture-positive and -negative histology. This feature could be of considerable interest because, as the sensitivity of organ culture seems to be greater than the initial histology, the new system might be useful in uncertain cases where the risk of missing the diagnosis of coeliac disease is high.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0036-5521 1502-7708
DOI:	10.1080/00365520510024151