Human single-chain antibodies that neutralize homologous and heterologous strains and clades of influenza A virus subtype H5N1

Human antibodies that interfere with the biological activity of haemagglutinins (HAs) of influenza viruses have high potential as an antiviral agent. Human single-chain antibody fragments (HuScFv) to recombinant and native HAs of the influenza virus H5N1 subtype were produced using a human antibody...

Full description

Saved in:

Bibliographic Details
Published in:	Antiviral therapy Vol. 14; no. 2; pp. 221 - 230
Main Authors:	MANEEWATCH, Santi, THANONGSAKSRIKUL, Jeeraphong, SONGSERM, Thaweesak, THUENG-IN, Kanyarat, KULKEAW, Kasem, THATHAISONG, Umapom, SRIMANOTE, Potjanee, TONGTAWE, Pongsri, TAPCHAISRI, Pramuan, CHAICUMPA, Wanpen
Format:	Journal Article
Language:	English
Published:	London International Medical Press 01-01-2009
Subjects:	Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antibodies, Monoclonal - administration & dosage Antibodies, Viral - administration & dosage Antiviral agents Biological and medical sciences Cell Line Dogs Hemagglutinin Glycoproteins, Influenza Virus - immunology Humans Immunization, Passive Immunoglobulin Fragments - administration & dosage Immunologic Factors - administration & dosage Influenza A virus Influenza A Virus, H5N1 Subtype - drug effects Influenza A Virus, H5N1 Subtype - immunology Influenza, Human - therapy Injections, Intraperitoneal Medical sciences Mice Pharmacology. Drug treatments Virus Human Influenzavirus A(H5N1) Influenzavirus A Typing Influenza A virus Orthomyxoviridae Homology Subtype Single chain antibody Strain
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Human antibodies that interfere with the biological activity of haemagglutinins (HAs) of influenza viruses have high potential as an antiviral agent. Human single-chain antibody fragments (HuScFv) to recombinant and native HAs of the influenza virus H5N1 subtype were produced using a human antibody phage display library with the intention to increase the therapeutic arsenal against this highly pathogenic virus. The HuScFv inhibited HA activity and neutralized infectivity of both homologous and heterologous strains and clades of the H5N1 subtype in Madin-Darby canine kidney cell cultures. Intraperitoneally injected HuScFv also mediated immunotherapeutic protection in mice that were intranasally challenged with highly pathogenic H5N1 viruses belonging to different strains and clades. Our data indicate that it might be worth pursuing these HuScFv further for future consideration as candidates for influenza intervention and treatment.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1359-6535 2040-2058
DOI:	10.1177/135965350901400204