Search Results - "TEDESCO, Donato"

Cancer cell-intrinsic resistance to BiTE therapy is mediated by loss of CD58 costimulation and modulation of the extrinsic apoptotic pathway by Shen, Ye, Eng, Jason S, Fajardo, Flordeliza, Liang, Lingming, Li, Cong, Collins, Patrick, Tedesco, Donato, Nolan-Stevaux, Olivier

“…BackgroundBispecific T-cell engager (BiTE) molecules induce redirected lysis of cancer cells by T cells and are an emerging modality for solid tumor…”
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The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein–ubiquitin ligase SCFSkp2 by Tedesco, Donato, Lukas, Jiri, Reed, Steven I.

“…p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of…”
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Measurement of Cancer Cell Growth Heterogeneity through Lentiviral Barcoding Identifies Clonal Dominance as a Characteristic of In Vivo Tumor Engraftment by Nolan-Stevaux, Olivier, Tedesco, Donato, Ragan, Seamus, Makhanov, Mikhail, Chenchik, Alex, Ruefli-Brasse, Astrid, Quon, Kim, Kassner, Paul D

“…Advances in the fields of cancer initiating cells and high-throughput in vivo shRNA screens have highlighted a need to observe the growth of tumor cells in…”
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Use of RNAi screens to uncover resistance mechanisms in cancer cells and identify synthetic lethal interactions by Diehl, Paul, Tedesco, Donato, Chenchik, Alex

“…RNAi loss-of-function screens, which have proven effective to identify genes functionally responsible for cellular phenotypes, can be designed to use different…”
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The Ubiquitin-Conjugating Enzyme E2-EPF Is Overexpressed in Primary Breast Cancer and Modulates Sensitivity to Topoisomerase II Inhibition by Tedesco, Donato, Zhang, Jianhuan, Trinht, Lan, Lalehzadeh, Guita, Meisner, Rene, Yamaguchi, Ken D., Ruderman, Daniel L., Dinter, Harald, Zajchowski, Deborah A.

“…We identified the ubiquitin-conjugating enzyme E2EPF mRNA as differentially expressed in breast tumors relative to normal tissues and performed studies to…”
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Cyclin E overexpression impairs progression through mitosis by inhibiting APCCdh1 by Keck, Jamie M., Summers, Matthew K., Tedesco, Donato, Ekholm-Reed, Susanna, Chuang, Li-Chiou, Jackson, Peter K., Reed, Steven I.

“…Overexpression of cyclin E, an activator of cyclin-dependent kinase 2, has been linked to human cancer. In cell culture models, the forced expression of cyclin…”
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MafK Mediates Chromatin Remodeling to Silence IRF8 Expression in Non-immune Cells in a Cell Type-SpecificManner by Fourier, Nitsan, Zolty, Maya, Azriel, Aviva, Tedesco, Donato, Levi, Ben-Zion

“…The regulation of gene expression is a result of a complex interplay between chromatin remodeling, transcription factors, and signaling molecules. Cell…”
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Abstract 2950: CRISPR/saCas9 and CRISPR/spCas9 systems for combinatorial genetic screens (CRISPR-KO, CRISPRa, CRISPRi) by Isachenko, Nadya, Aleksanyan, Gayane, Diehl, Paul, Tedesco, Donato

“…Abstract This study explores the utilization of orthogonal CRISPR-based gene editing/modulation systems for combinatorial genetic screens using CRISPR knockout…”
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Abstract 5868: Cell barcoding, genetic screens and expression profiling at a single-cell level by Chenchik, Alex, Makhanov, Mikhail, Darst, Russell, Deng, Debbie, Tedesco, Donato, Diehl, Paul, Kobzik, Lester

“…Abstract Novel single-cell methods for profiling clonal composition and phenotypes of genetically modified cancer cells may improve understanding of…”
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Abstract 403: CRISPR/Cas9 genome-wide sgRNA libraries for loss-of-function and gain-of-function genetic screens by Tedesco, Donato, Diehl, Paul, Makhanov, Mikhail, Baron, Sylvain, Chenchik, Alex

“…Abstract Genome-wide loss-of-function screening is a fundamental method to identify genes responsible for driving biological processes. Complex pooled…”
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The pRb-related protein p130 is regulated by phosphorylation-dependent proteolysis via the protein-ubiquitin ligase SCF(Skp2) by Tedesco, Donato, Lukas, Jiri, Reed, Steven I

“…p130 is a tumor suppressor of the pocket protein family whose expression is posttranscriptionally regulated and largely G0 restricted. The mechanism of…”
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Abstract 2949: A flexible and efficient approach for single-cell CRISPR perturbation screens combined with targeted RNA-seq expression analysis by Tedesco, Donato, Liu, Tianbing, Makhanov, Mikhail, Hu, Dongfang, Isachenko, Nadya, Diehl, Paul, Chenchik, Alex

“…Abstract This study presents a novel approach for performing 10X platform-based targeted single-cell RNA-seq analysis in combination with CRISPR perturbation…”
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Abstract 1112: Loss-of-function genetic screening with shRNA and CRISPR libraries by Tedesco, Donato, Makhanov, Mikhail, Baron, Sylvain, Suchkov, Dmitry, Chenchik, Alex

“…Abstract Genome-wide loss-of-function screens provide a direct approach to identify the genes regulating biological responses and find new therapeutic targets…”
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MDM4 Is Targeted by 1q Gain and Drives Disease in Burkitt Lymphoma by Hüllein, Jennifer, Słabicki, Mikołaj, Rosolowski, Maciej, Jethwa, Alexander, Habringer, Stefan, Tomska, Katarzyna, Kurilov, Roma, Lu, Junyan, Scheinost, Sebastian, Wagener, Rabea, Huang, Zhiqin, Lukas, Marina, Yavorska, Olena, Helfrich, Hanne, Scholtysik, René, Bonneau, Kyle, Tedesco, Donato, Küppers, Ralf, Klapper, Wolfram, Pott, Christiane, Stilgenbauer, Stephan, Burkhardt, Birgit, Löffler, Markus, Trümper, Lorenz H, Hummel, Michael, Brors, Benedikt, Zapatka, Marc, Siebert, Reiner, Kreuz, Markus, Keller, Ulrich, Huber, Wolfgang, Zenz, Thorsten

“…Oncogenic MYC activation promotes proliferation in Burkitt lymphoma, but also induces cell-cycle arrest and apoptosis mediated by p53, a tumor suppressor that…”
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Abstract 4354: CRISPR/Cas9 genome-wide gRNA library for target identification by Tedesco, Donato, Diehl, Paul, Makhanov, Mikhail, Baron, Sylvain, Suchkov, Dmitry, Frangou, Costa, Chenchik, Alex

“…Abstract Genome-wide loss-of-function screening is a fundamental method to identify genes responsible for driving biological responses, and complex pooled…”
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Abstract 4354: CRISPRCas9 genome-wide gRNA library for target identification by Tedesco, Donato, Diehl, Paul, Makhanov, Mikhail, Baron, Sylvain, Suchkov, Dmitry, Frangou, Costa, Chenchik, Alex

“…Genome-wide loss-of-function screening is a fundamental method to identify genes responsible for driving biological responses, and complex pooled…”
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Abstract 2946: Perturb-seq analysis of TNFα-induced transcriptional response by Tedesco, Donato, Chenchik, Alex, Liu, Tianbing, Hu, Dongfang, Isachenko, Nadya, Dolganov, Nadia

“…Abstract We performed a single-cell Perturb-seq screen on HEK293 cells with a ~100 sgRNA pooled library, aimed at identifying genes involved in TNFα-induced…”
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Abstract 1721: Moving beyond in vitro models and addressing the challenges of pooled RNAi screens in mouse xenografts by Diehl, Paul, Tedesco, Donato, Bonneau, Kyle, Makhanov, Mikhail, Deng, Debbie, Sun, Peiqing, Chenchik, Alex

“…Abstract RNAi loss-of-function screening with pooled shRNA expression libraries has proven to be a useful tool to identify essential cancer genes and drug…”
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Deregulation of Cyclin E in Human Cells Interferes with Prereplication Complex Assembly by Ekholm-Reed, Susanna, Méndez, Juan, Tedesco, Donato, Zetterberg, Anders, Stillman, Bruce, Reed, Steven I.

“…Deregulation of cyclin E expression has been associated with a broad spectrum of human malignancies. Analysis of DNA replication in cells constitutively…”
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Abstract C161: CRISPR/Cas9 genome-wide gRNA library screening platform by Tedesco, Donato, Diehl, Paul, Makhanov, Mikhail, Baron, Sylvain, Suchkov, Dmitry, Chenchik, Alex

“…Abstract CRISPR/Cas9 gene knock-out technology can be used as a powerful tool for large-scale functional genomic analysis in mammalian cells. With the goal to…”
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